Asymmetric localization of DLC1 defines avian trunk neural crest polarity for directional delamination and migration

Jessica Aijia Liu, Yanxia Rao, May Pui Lai Cheung, Man Ning Hui, Ming Hoi Wu, Lo Kong Chan, Irene Oi Lin Ng, Ben Niu, Kathryn S.E. Cheah, Rakesh Sharma, Louis Hodgson, Martin Cheung

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Following epithelial-mesenchymal transition, acquisition of avian trunk neural crest cell (NCC) polarity is prerequisite for directional delamination and migration, which in turn is essential for peripheral nervous system development. However, how this cell polarization is established and regulated remains unknown. Here we demonstrate that, using the RHOA biosensor in vivo and in vitro, the initiation of NCC polarization is accompanied by highly activated RHOA in the cytoplasm at the cell rear and its fluctuating activity at the front edge. This differential RHOA activity determines polarized NC morphology and motility, and is regulated by the asymmetrically localized RhoGAP Deleted in liver cancer (DLC1) in the cytoplasm at the cell front. Importantly, the association of DLC1 with NEDD9 is crucial for its asymmetric localization and differential RHOA activity. Moreover, NC specifiers, SOX9 and SOX10, regulate NEDD9 and DLC1 expression, respectively. These results present a SOX9/SOX10-NEDD9/DLC1-RHOA regulatory axis to govern NCC migratory polarization.

Original languageEnglish (US)
Article number1185
JournalNature Communications
Volume8
Issue number1
DOIs
StatePublished - Dec 1 2017

Fingerprint

Neural Crest
Delamination
polarity
Polarization
Cytoplasm
cells
Cell Polarity
Epithelial-Mesenchymal Transition
cytoplasm
Peripheral Nervous System
Neurology
Biosensing Techniques
Liver Neoplasms
Biosensors
Liver
polarization
peripheral nervous system
locomotion
bioinstrumentation
liver

ASJC Scopus subject areas

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Asymmetric localization of DLC1 defines avian trunk neural crest polarity for directional delamination and migration. / Liu, Jessica Aijia; Rao, Yanxia; Cheung, May Pui Lai; Hui, Man Ning; Wu, Ming Hoi; Chan, Lo Kong; Ng, Irene Oi Lin; Niu, Ben; Cheah, Kathryn S.E.; Sharma, Rakesh; Hodgson, Louis; Cheung, Martin.

In: Nature Communications, Vol. 8, No. 1, 1185, 01.12.2017.

Research output: Contribution to journalArticle

Liu, JA, Rao, Y, Cheung, MPL, Hui, MN, Wu, MH, Chan, LK, Ng, IOL, Niu, B, Cheah, KSE, Sharma, R, Hodgson, L & Cheung, M 2017, 'Asymmetric localization of DLC1 defines avian trunk neural crest polarity for directional delamination and migration', Nature Communications, vol. 8, no. 1, 1185. https://doi.org/10.1038/s41467-017-01107-0
Liu, Jessica Aijia ; Rao, Yanxia ; Cheung, May Pui Lai ; Hui, Man Ning ; Wu, Ming Hoi ; Chan, Lo Kong ; Ng, Irene Oi Lin ; Niu, Ben ; Cheah, Kathryn S.E. ; Sharma, Rakesh ; Hodgson, Louis ; Cheung, Martin. / Asymmetric localization of DLC1 defines avian trunk neural crest polarity for directional delamination and migration. In: Nature Communications. 2017 ; Vol. 8, No. 1.
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