Astrocyte end-feet in germinal matrix, cerebral cortex, and white matter in developing infants

Nadine El-Khoury, Alex Braun, Furong Hu, Maya Pandey, Maiken Nedergaard, Edmund F. Lagamma, Praveen Ballabh

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Astrocyte end-feet ensheathe blood vessels in the brain and are believed to provide structural integrity to the cerebral vasculature. We sought to determine in developing infants whether the coverage of blood vessels by astrocyte end-feet is decreased in germinal matrix (GM) compared with cerebral cortex and white matter (WM), which may cause fragility of the GM vasculature. Therefore, we evaluated the perivascular coverage by astrocyte end-feet in these areas. We double-labeled the brain sections with astroglial markers [glial fibrillary acidic protein (GFAP), aquaporin-4 (AQP4), and S-100β] and a vascular marker, laminin. Perivascular coverage by GFAP+ astrocyte end-feet increased consistently as a function of gestational age (GA) in cortex and WM from 19 to 40 wk. Compared with GFAP, AQP4+ astrocyte end-feet developed at an earlier GA, ensheathing about 63% of blood vessels for 23-40 wk in cortex, WM, and GM. Coverage by GFAP+ perivascular end-feet was decreased in GM compared with cortex and WM from 23 to 34 wk. There was no difference in the coverage by AQP4+ end-feet among the three areas in these infants. The expression of AQP4, a water channel molecule, in the astrocyte end-feet was not significantly different between premature and mature infants, suggesting similar risk of brain edema in preterm and term infants in pathologic conditions. More importantly, the lesser degree of GFAP expression in astrocyte end-feet of GM compared with cortex and WM may reflect a cytoskeletal structural difference that contributes to the fragility of GM vasculature and propensity to hemorrhage.

Original languageEnglish (US)
Pages (from-to)673-679
Number of pages7
JournalPediatric Research
Volume59
Issue number5
DOIs
StatePublished - May 2006
Externally publishedYes

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Astrocytes
Cerebral Cortex
Aquaporin 4
Glial Fibrillary Acidic Protein
Blood Vessels
Premature Infants
Gestational Age
Aquaporins
Brain Edema
Brain
Laminin
White Matter
Hemorrhage

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Astrocyte end-feet in germinal matrix, cerebral cortex, and white matter in developing infants. / El-Khoury, Nadine; Braun, Alex; Hu, Furong; Pandey, Maya; Nedergaard, Maiken; Lagamma, Edmund F.; Ballabh, Praveen.

In: Pediatric Research, Vol. 59, No. 5, 05.2006, p. 673-679.

Research output: Contribution to journalArticle

El-Khoury, Nadine ; Braun, Alex ; Hu, Furong ; Pandey, Maya ; Nedergaard, Maiken ; Lagamma, Edmund F. ; Ballabh, Praveen. / Astrocyte end-feet in germinal matrix, cerebral cortex, and white matter in developing infants. In: Pediatric Research. 2006 ; Vol. 59, No. 5. pp. 673-679.
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