Association of poly(A) mRNA with microtubules in cultured neurons

Gary J. Bassell, Robert H. Singer, Kenneth S. Kosik

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

The structural basis for the synthesis of specific proteins within distinct intraneuronal compartments is unknown. We studied the distribution of poly(A) mRNA within cultured cerebrocortical neurons using high resolution in situ hybridization to identify cytoskeletal components that may anchor mRNA. After 1 day in culture, poly(A) mRNA was distributed throughout all of the initial neurites, including the axon-like process. At 4 days in culture, poly(A) mRNA was distributed throughout the cell body and dendritic processes, but confined to the proximal segment of the axon. Poly(A) mRNA was bound to the cytoskeleton as demonstrated by resistance to detergent extraction. Perturbation of microtubules with colchicine resulted in a major reduction of dendritic poly(A) mRNA; however, this distribution was unaffected by cytochalasin. Ultrastructural in situ hybridization revealed that poly(A) mRNA and associated ribosomes were excluded from tightly bundled microtubules.

Original languageEnglish (US)
Pages (from-to)571-582
Number of pages12
JournalNeuron
Volume12
Issue number3
DOIs
StatePublished - 1994
Externally publishedYes

Fingerprint

Microtubules
Neurons
Messenger RNA
In Situ Hybridization
Axons
Cytochalasins
Colchicine
Neurites
Cytoskeleton
Ribosomes
Detergents
Proteins

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Association of poly(A) mRNA with microtubules in cultured neurons. / Bassell, Gary J.; Singer, Robert H.; Kosik, Kenneth S.

In: Neuron, Vol. 12, No. 3, 1994, p. 571-582.

Research output: Contribution to journalArticle

Bassell, Gary J. ; Singer, Robert H. ; Kosik, Kenneth S. / Association of poly(A) mRNA with microtubules in cultured neurons. In: Neuron. 1994 ; Vol. 12, No. 3. pp. 571-582.
@article{555a546af0294abeaca50771adb4a252,
title = "Association of poly(A) mRNA with microtubules in cultured neurons",
abstract = "The structural basis for the synthesis of specific proteins within distinct intraneuronal compartments is unknown. We studied the distribution of poly(A) mRNA within cultured cerebrocortical neurons using high resolution in situ hybridization to identify cytoskeletal components that may anchor mRNA. After 1 day in culture, poly(A) mRNA was distributed throughout all of the initial neurites, including the axon-like process. At 4 days in culture, poly(A) mRNA was distributed throughout the cell body and dendritic processes, but confined to the proximal segment of the axon. Poly(A) mRNA was bound to the cytoskeleton as demonstrated by resistance to detergent extraction. Perturbation of microtubules with colchicine resulted in a major reduction of dendritic poly(A) mRNA; however, this distribution was unaffected by cytochalasin. Ultrastructural in situ hybridization revealed that poly(A) mRNA and associated ribosomes were excluded from tightly bundled microtubules.",
author = "Bassell, {Gary J.} and Singer, {Robert H.} and Kosik, {Kenneth S.}",
year = "1994",
doi = "10.1016/0896-6273(94)90213-5",
language = "English (US)",
volume = "12",
pages = "571--582",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "3",

}

TY - JOUR

T1 - Association of poly(A) mRNA with microtubules in cultured neurons

AU - Bassell, Gary J.

AU - Singer, Robert H.

AU - Kosik, Kenneth S.

PY - 1994

Y1 - 1994

N2 - The structural basis for the synthesis of specific proteins within distinct intraneuronal compartments is unknown. We studied the distribution of poly(A) mRNA within cultured cerebrocortical neurons using high resolution in situ hybridization to identify cytoskeletal components that may anchor mRNA. After 1 day in culture, poly(A) mRNA was distributed throughout all of the initial neurites, including the axon-like process. At 4 days in culture, poly(A) mRNA was distributed throughout the cell body and dendritic processes, but confined to the proximal segment of the axon. Poly(A) mRNA was bound to the cytoskeleton as demonstrated by resistance to detergent extraction. Perturbation of microtubules with colchicine resulted in a major reduction of dendritic poly(A) mRNA; however, this distribution was unaffected by cytochalasin. Ultrastructural in situ hybridization revealed that poly(A) mRNA and associated ribosomes were excluded from tightly bundled microtubules.

AB - The structural basis for the synthesis of specific proteins within distinct intraneuronal compartments is unknown. We studied the distribution of poly(A) mRNA within cultured cerebrocortical neurons using high resolution in situ hybridization to identify cytoskeletal components that may anchor mRNA. After 1 day in culture, poly(A) mRNA was distributed throughout all of the initial neurites, including the axon-like process. At 4 days in culture, poly(A) mRNA was distributed throughout the cell body and dendritic processes, but confined to the proximal segment of the axon. Poly(A) mRNA was bound to the cytoskeleton as demonstrated by resistance to detergent extraction. Perturbation of microtubules with colchicine resulted in a major reduction of dendritic poly(A) mRNA; however, this distribution was unaffected by cytochalasin. Ultrastructural in situ hybridization revealed that poly(A) mRNA and associated ribosomes were excluded from tightly bundled microtubules.

UR - http://www.scopus.com/inward/record.url?scp=0028214453&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028214453&partnerID=8YFLogxK

U2 - 10.1016/0896-6273(94)90213-5

DO - 10.1016/0896-6273(94)90213-5

M3 - Article

VL - 12

SP - 571

EP - 582

JO - Neuron

JF - Neuron

SN - 0896-6273

IS - 3

ER -