Association of poly(A) mRNA with microtubules in cultured neurons

Gary J. Bassell; Robert H. Singer; Kenneth S. Kosik

doi:10.1016/0896-6273(94)90213-5

Association of poly(A) mRNA with microtubules in cultured neurons

Gary J. Bassell, Robert H. Singer, Kenneth S. Kosik

Research output: Contribution to journal › Article › peer-review

126 Scopus citations

Abstract

The structural basis for the synthesis of specific proteins within distinct intraneuronal compartments is unknown. We studied the distribution of poly(A) mRNA within cultured cerebrocortical neurons using high resolution in situ hybridization to identify cytoskeletal components that may anchor mRNA. After 1 day in culture, poly(A) mRNA was distributed throughout all of the initial neurites, including the axon-like process. At 4 days in culture, poly(A) mRNA was distributed throughout the cell body and dendritic processes, but confined to the proximal segment of the axon. Poly(A) mRNA was bound to the cytoskeleton as demonstrated by resistance to detergent extraction. Perturbation of microtubules with colchicine resulted in a major reduction of dendritic poly(A) mRNA; however, this distribution was unaffected by cytochalasin. Ultrastructural in situ hybridization revealed that poly(A) mRNA and associated ribosomes were excluded from tightly bundled microtubules.

Original language	English (US)
Pages (from-to)	571-582
Number of pages	12
Journal	Neuron
Volume	12
Issue number	3
DOIs	https://doi.org/10.1016/0896-6273(94)90213-5
State	Published - Mar 1994
Externally published	Yes

ASJC Scopus subject areas

General Neuroscience

Access to Document

10.1016/0896-6273(94)90213-5

Cite this

@article{555a546af0294abeaca50771adb4a252,

title = "Association of poly(A) mRNA with microtubules in cultured neurons",

abstract = "The structural basis for the synthesis of specific proteins within distinct intraneuronal compartments is unknown. We studied the distribution of poly(A) mRNA within cultured cerebrocortical neurons using high resolution in situ hybridization to identify cytoskeletal components that may anchor mRNA. After 1 day in culture, poly(A) mRNA was distributed throughout all of the initial neurites, including the axon-like process. At 4 days in culture, poly(A) mRNA was distributed throughout the cell body and dendritic processes, but confined to the proximal segment of the axon. Poly(A) mRNA was bound to the cytoskeleton as demonstrated by resistance to detergent extraction. Perturbation of microtubules with colchicine resulted in a major reduction of dendritic poly(A) mRNA; however, this distribution was unaffected by cytochalasin. Ultrastructural in situ hybridization revealed that poly(A) mRNA and associated ribosomes were excluded from tightly bundled microtubules.",

author = "Bassell, {Gary J.} and Singer, {Robert H.} and Kosik, {Kenneth S.}",

note = "Funding Information: tance with neuronal cultures and numerous helpful discussions. We thank Lester Binder for antibodies to MAP2 and Anthony Frankfurter for antibody to class III tubulin. We thank Christine Powers and Peter Paskovitch for electron microscopy assistance. This work was supported by a grant from the Alzheimer's Disease and Related Disorders Association to G. J. B. and National Institutes of Health grants AG06601 and NS29031 to K. S. K.",

year = "1994",

month = mar,

doi = "10.1016/0896-6273(94)90213-5",

language = "English (US)",

volume = "12",

pages = "571--582",

journal = "Neuron",

issn = "0896-6273",

publisher = "Cell Press",

number = "3",

}

TY - JOUR

T1 - Association of poly(A) mRNA with microtubules in cultured neurons

AU - Bassell, Gary J.

AU - Singer, Robert H.

AU - Kosik, Kenneth S.

N1 - Funding Information: tance with neuronal cultures and numerous helpful discussions. We thank Lester Binder for antibodies to MAP2 and Anthony Frankfurter for antibody to class III tubulin. We thank Christine Powers and Peter Paskovitch for electron microscopy assistance. This work was supported by a grant from the Alzheimer's Disease and Related Disorders Association to G. J. B. and National Institutes of Health grants AG06601 and NS29031 to K. S. K.

PY - 1994/3

Y1 - 1994/3

N2 - The structural basis for the synthesis of specific proteins within distinct intraneuronal compartments is unknown. We studied the distribution of poly(A) mRNA within cultured cerebrocortical neurons using high resolution in situ hybridization to identify cytoskeletal components that may anchor mRNA. After 1 day in culture, poly(A) mRNA was distributed throughout all of the initial neurites, including the axon-like process. At 4 days in culture, poly(A) mRNA was distributed throughout the cell body and dendritic processes, but confined to the proximal segment of the axon. Poly(A) mRNA was bound to the cytoskeleton as demonstrated by resistance to detergent extraction. Perturbation of microtubules with colchicine resulted in a major reduction of dendritic poly(A) mRNA; however, this distribution was unaffected by cytochalasin. Ultrastructural in situ hybridization revealed that poly(A) mRNA and associated ribosomes were excluded from tightly bundled microtubules.

AB - The structural basis for the synthesis of specific proteins within distinct intraneuronal compartments is unknown. We studied the distribution of poly(A) mRNA within cultured cerebrocortical neurons using high resolution in situ hybridization to identify cytoskeletal components that may anchor mRNA. After 1 day in culture, poly(A) mRNA was distributed throughout all of the initial neurites, including the axon-like process. At 4 days in culture, poly(A) mRNA was distributed throughout the cell body and dendritic processes, but confined to the proximal segment of the axon. Poly(A) mRNA was bound to the cytoskeleton as demonstrated by resistance to detergent extraction. Perturbation of microtubules with colchicine resulted in a major reduction of dendritic poly(A) mRNA; however, this distribution was unaffected by cytochalasin. Ultrastructural in situ hybridization revealed that poly(A) mRNA and associated ribosomes were excluded from tightly bundled microtubules.

UR - http://www.scopus.com/inward/record.url?scp=0028214453&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028214453&partnerID=8YFLogxK

U2 - 10.1016/0896-6273(94)90213-5

DO - 10.1016/0896-6273(94)90213-5

M3 - Article

C2 - 8155320

AN - SCOPUS:0028214453

SN - 0896-6273

VL - 12

SP - 571

EP - 582

JO - Neuron

JF - Neuron

IS - 3

ER -

Association of poly(A) mRNA with microtubules in cultured neurons

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this