TY - JOUR
T1 - Application of PCR-RFLP for the exploration of the molecular diversity of Leishmania infantum in Algeria
AU - Seridi, Nabila
AU - Belkaid, Miloud
AU - Quispe-Tintaya, Wilber
AU - Zidane, Charef
AU - Dujardin, Jean Claude
N1 - Funding Information:
This work was supported by the European Commission (Contracts QLK2-CT-2001-01810 and INCO-CT-2004-509086) and by a research fund from the University of Sciences and Technology, Houari Boumediene (USTHB) and from Institut Pasteur d’Algérie (IPA).
PY - 2008/6
Y1 - 2008/6
N2 - In recent years, new methods have been developed for the molecular typing of Leishmania that need to be extensively validated by studies of clinical isolates in a well defined epidemiological context. The present study is a contribution to this effort. Using PCR-RFLP of gp63 and cpb genes, we analysed 59 isolates of L. (L.) infantum obtained from different regions of Algeria and originating from different clinical forms, hosts and zymodemes. PCR-RFLP identified 15 different genotypes among the four zymodemes analysed, thereby demonstrating a higher discriminatory power than multilocus enzyme electrophoresis. We did not see any significant relationships between PCR-RFLP patterns and host origin. However, cpb polymorphism showed two interesting trends: a possible relationship with the cutaneous origin of the isolates and an association with a West-East cline. We verified the proof of evidence of the direct applicability of gp63 and cpb PCR-RFLP in blood samples from dogs. Further work is needed to compare the sensitivity of pattern detection with cpb and gp63 PCR-RFLP but our results pave the way to future multilocus PCR-RFLP studies of L. (L.) infantum populations.
AB - In recent years, new methods have been developed for the molecular typing of Leishmania that need to be extensively validated by studies of clinical isolates in a well defined epidemiological context. The present study is a contribution to this effort. Using PCR-RFLP of gp63 and cpb genes, we analysed 59 isolates of L. (L.) infantum obtained from different regions of Algeria and originating from different clinical forms, hosts and zymodemes. PCR-RFLP identified 15 different genotypes among the four zymodemes analysed, thereby demonstrating a higher discriminatory power than multilocus enzyme electrophoresis. We did not see any significant relationships between PCR-RFLP patterns and host origin. However, cpb polymorphism showed two interesting trends: a possible relationship with the cutaneous origin of the isolates and an association with a West-East cline. We verified the proof of evidence of the direct applicability of gp63 and cpb PCR-RFLP in blood samples from dogs. Further work is needed to compare the sensitivity of pattern detection with cpb and gp63 PCR-RFLP but our results pave the way to future multilocus PCR-RFLP studies of L. (L.) infantum populations.
KW - Algeria
KW - Leishmania infantum
KW - Leishmaniasis
KW - PCR
KW - Polymorphism
KW - RFLP
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U2 - 10.1016/j.trstmh.2008.03.004
DO - 10.1016/j.trstmh.2008.03.004
M3 - Article
C2 - 18407304
AN - SCOPUS:43149104364
SN - 0035-9203
VL - 102
SP - 556
EP - 563
JO - Transactions of the Royal Society of Tropical Medicine and Hygiene
JF - Transactions of the Royal Society of Tropical Medicine and Hygiene
IS - 6
ER -