Application of PCR-RFLP for the exploration of the molecular diversity of Leishmania infantum in Algeria

Nabila Seridi, Miloud Belkaid, Wilber Quispe-Tintaya, Charef Zidane, Jean Claude Dujardin

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

In recent years, new methods have been developed for the molecular typing of Leishmania that need to be extensively validated by studies of clinical isolates in a well defined epidemiological context. The present study is a contribution to this effort. Using PCR-RFLP of gp63 and cpb genes, we analysed 59 isolates of L. (L.) infantum obtained from different regions of Algeria and originating from different clinical forms, hosts and zymodemes. PCR-RFLP identified 15 different genotypes among the four zymodemes analysed, thereby demonstrating a higher discriminatory power than multilocus enzyme electrophoresis. We did not see any significant relationships between PCR-RFLP patterns and host origin. However, cpb polymorphism showed two interesting trends: a possible relationship with the cutaneous origin of the isolates and an association with a West-East cline. We verified the proof of evidence of the direct applicability of gp63 and cpb PCR-RFLP in blood samples from dogs. Further work is needed to compare the sensitivity of pattern detection with cpb and gp63 PCR-RFLP but our results pave the way to future multilocus PCR-RFLP studies of L. (L.) infantum populations.

Original languageEnglish (US)
Pages (from-to)556-563
Number of pages8
JournalTransactions of the Royal Society of Tropical Medicine and Hygiene
Volume102
Issue number6
DOIs
StatePublished - Jun 2008
Externally publishedYes

Fingerprint

Leishmania infantum
Algeria
Restriction Fragment Length Polymorphisms
Polymerase Chain Reaction
Molecular Typing
Leishmania
Electrophoresis
Genotype
Dogs
Skin
Enzymes
Population
Genes

Keywords

  • Algeria
  • Leishmania infantum
  • Leishmaniasis
  • PCR
  • Polymorphism
  • RFLP

ASJC Scopus subject areas

  • Parasitology
  • Medicine(all)

Cite this

Application of PCR-RFLP for the exploration of the molecular diversity of Leishmania infantum in Algeria. / Seridi, Nabila; Belkaid, Miloud; Quispe-Tintaya, Wilber; Zidane, Charef; Dujardin, Jean Claude.

In: Transactions of the Royal Society of Tropical Medicine and Hygiene, Vol. 102, No. 6, 06.2008, p. 556-563.

Research output: Contribution to journalArticle

Seridi, Nabila ; Belkaid, Miloud ; Quispe-Tintaya, Wilber ; Zidane, Charef ; Dujardin, Jean Claude. / Application of PCR-RFLP for the exploration of the molecular diversity of Leishmania infantum in Algeria. In: Transactions of the Royal Society of Tropical Medicine and Hygiene. 2008 ; Vol. 102, No. 6. pp. 556-563.
@article{49b5e8b350b840398ac52cb36d924d36,
title = "Application of PCR-RFLP for the exploration of the molecular diversity of Leishmania infantum in Algeria",
abstract = "In recent years, new methods have been developed for the molecular typing of Leishmania that need to be extensively validated by studies of clinical isolates in a well defined epidemiological context. The present study is a contribution to this effort. Using PCR-RFLP of gp63 and cpb genes, we analysed 59 isolates of L. (L.) infantum obtained from different regions of Algeria and originating from different clinical forms, hosts and zymodemes. PCR-RFLP identified 15 different genotypes among the four zymodemes analysed, thereby demonstrating a higher discriminatory power than multilocus enzyme electrophoresis. We did not see any significant relationships between PCR-RFLP patterns and host origin. However, cpb polymorphism showed two interesting trends: a possible relationship with the cutaneous origin of the isolates and an association with a West-East cline. We verified the proof of evidence of the direct applicability of gp63 and cpb PCR-RFLP in blood samples from dogs. Further work is needed to compare the sensitivity of pattern detection with cpb and gp63 PCR-RFLP but our results pave the way to future multilocus PCR-RFLP studies of L. (L.) infantum populations.",
keywords = "Algeria, Leishmania infantum, Leishmaniasis, PCR, Polymorphism, RFLP",
author = "Nabila Seridi and Miloud Belkaid and Wilber Quispe-Tintaya and Charef Zidane and Dujardin, {Jean Claude}",
year = "2008",
month = "6",
doi = "10.1016/j.trstmh.2008.03.004",
language = "English (US)",
volume = "102",
pages = "556--563",
journal = "Transactions of the Royal Society of Tropical Medicine and Hygiene",
issn = "0035-9203",
publisher = "Elsevier",
number = "6",

}

TY - JOUR

T1 - Application of PCR-RFLP for the exploration of the molecular diversity of Leishmania infantum in Algeria

AU - Seridi, Nabila

AU - Belkaid, Miloud

AU - Quispe-Tintaya, Wilber

AU - Zidane, Charef

AU - Dujardin, Jean Claude

PY - 2008/6

Y1 - 2008/6

N2 - In recent years, new methods have been developed for the molecular typing of Leishmania that need to be extensively validated by studies of clinical isolates in a well defined epidemiological context. The present study is a contribution to this effort. Using PCR-RFLP of gp63 and cpb genes, we analysed 59 isolates of L. (L.) infantum obtained from different regions of Algeria and originating from different clinical forms, hosts and zymodemes. PCR-RFLP identified 15 different genotypes among the four zymodemes analysed, thereby demonstrating a higher discriminatory power than multilocus enzyme electrophoresis. We did not see any significant relationships between PCR-RFLP patterns and host origin. However, cpb polymorphism showed two interesting trends: a possible relationship with the cutaneous origin of the isolates and an association with a West-East cline. We verified the proof of evidence of the direct applicability of gp63 and cpb PCR-RFLP in blood samples from dogs. Further work is needed to compare the sensitivity of pattern detection with cpb and gp63 PCR-RFLP but our results pave the way to future multilocus PCR-RFLP studies of L. (L.) infantum populations.

AB - In recent years, new methods have been developed for the molecular typing of Leishmania that need to be extensively validated by studies of clinical isolates in a well defined epidemiological context. The present study is a contribution to this effort. Using PCR-RFLP of gp63 and cpb genes, we analysed 59 isolates of L. (L.) infantum obtained from different regions of Algeria and originating from different clinical forms, hosts and zymodemes. PCR-RFLP identified 15 different genotypes among the four zymodemes analysed, thereby demonstrating a higher discriminatory power than multilocus enzyme electrophoresis. We did not see any significant relationships between PCR-RFLP patterns and host origin. However, cpb polymorphism showed two interesting trends: a possible relationship with the cutaneous origin of the isolates and an association with a West-East cline. We verified the proof of evidence of the direct applicability of gp63 and cpb PCR-RFLP in blood samples from dogs. Further work is needed to compare the sensitivity of pattern detection with cpb and gp63 PCR-RFLP but our results pave the way to future multilocus PCR-RFLP studies of L. (L.) infantum populations.

KW - Algeria

KW - Leishmania infantum

KW - Leishmaniasis

KW - PCR

KW - Polymorphism

KW - RFLP

UR - http://www.scopus.com/inward/record.url?scp=43149104364&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=43149104364&partnerID=8YFLogxK

U2 - 10.1016/j.trstmh.2008.03.004

DO - 10.1016/j.trstmh.2008.03.004

M3 - Article

C2 - 18407304

AN - SCOPUS:43149104364

VL - 102

SP - 556

EP - 563

JO - Transactions of the Royal Society of Tropical Medicine and Hygiene

JF - Transactions of the Royal Society of Tropical Medicine and Hygiene

SN - 0035-9203

IS - 6

ER -