Application of Fluorescence Microscopy and Behavioral Assays to Demonstrating Neuronal Connectomes and Neurotransmitter Systems in C. elegans

Omamuyovwi M. Ijomone, Priscila Gubert, Comfort O.A. Okoh, Alexandre M. Varão, Leandro de O. Amaral, Oritoke M. Aluko, Michael Aschner

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The nematode Caenorhabditis elegans (C. elegans) is a prevailing model commonly utilized in a variety of biomedical research fields, including neuroscience. Due to its transparency and simplicity, it is becoming a choice model organism for conducting imaging and behavioral assessment crucial to understanding the intricacies of the nervous system. Here, the methods required for neuronal characterization using fluorescent proteins and behavioral tasks are described. These are simplified protocols using fluorescent microscopy and behavioral assays to examine neuronal connections and associated neurotransmitter systems involved in normal physiology and aberrant pathology of the nervous system. Here, we aim is to make available to readers some streamlined and replicable procedures using the C. elegans models as well as highlighting some of the limitations.

Original languageEnglish (US)
Title of host publicationNeuromethods
PublisherHumana Press Inc.
Pages399-426
Number of pages28
DOIs
StatePublished - 2021

Publication series

NameNeuromethods
Volume172
ISSN (Print)0893-2336
ISSN (Electronic)1940-6045

Keywords

  • Behavior
  • C. elegans
  • Connectomes
  • Fluorescence microscopy
  • Neurotransmitters

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Psychiatry and Mental health

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