@inbook{81b0b87ee4b941bcbd788df19c9606a6,
title = "Application of Fluorescence Microscopy and Behavioral Assays to Demonstrating Neuronal Connectomes and Neurotransmitter Systems in C. elegans",
abstract = "The nematode Caenorhabditis elegans (C. elegans) is a prevailing model commonly utilized in a variety of biomedical research fields, including neuroscience. Due to its transparency and simplicity, it is becoming a choice model organism for conducting imaging and behavioral assessment crucial to understanding the intricacies of the nervous system. Here, the methods required for neuronal characterization using fluorescent proteins and behavioral tasks are described. These are simplified protocols using fluorescent microscopy and behavioral assays to examine neuronal connections and associated neurotransmitter systems involved in normal physiology and aberrant pathology of the nervous system. Here, we aim is to make available to readers some streamlined and replicable procedures using the C. elegans models as well as highlighting some of the limitations.",
keywords = "Behavior, C. elegans, Connectomes, Fluorescence microscopy, Neurotransmitters",
author = "Ijomone, {Omamuyovwi M.} and Priscila Gubert and Okoh, {Comfort O.A.} and Var{\~a}o, {Alexandre M.} and Amaral, {Leandro de O.} and Aluko, {Oritoke M.} and Michael Aschner",
note = "Funding Information: OMI acknowledges the 2019 Young IBRO Regions Connecting Awards. MA is supported by National Institute of Health (NIH), USA grants, NIEHS R01 10563, NIEHS R01 07331, and NIEHS R01 020852. We acknowledge Tao Ke of the Albert Einstein College of Medicine for images of C. elegans dopaminergic neurodegeneration under MeHg exposure. Publisher Copyright: {\textcopyright} 2021, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2021",
doi = "10.1007/978-1-0716-1637-6_18",
language = "English (US)",
series = "Neuromethods",
publisher = "Humana Press Inc.",
pages = "399--426",
booktitle = "Neuromethods",
}
