Antiserum raised against residues 159-168 of the guanine nucleotide-binding protein G(i3)-α reacts with ependymal cells and some neurons in the rat brain containing cholecystokinin- or cholecystokinin- and tyrosine 3-hydroxylase-like immunoreactivities

R. Cortes, T. Hokfelt, M. Schalling, M. Goldstein, P. Goldsmith, Allen M. Spiegel, C. Unson, J. Walsh

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Abstract

Antibodies raised against a synthetic decapeptide corresponding to a specific sequence of G(i3)-α protein (an inhibitory guanine nucleotide-binding protein) were used to analyze G(i3)-α-like immunoreactivity in brain sections from colchicine-treated rats by indirect immunofluorescence histochemistry. G(i3)-α-peptide-positive cell bodies were found in the ventral tegmental area and substantia nigra, and these cells were also cholecystokinin (CCK)- and tyrosine 3-hydroxylase-positive. G(i3)-α-peptide staining was observed in perikarya in the hippocampus and in fibers in the nucleus accumbens, tuberculum olfactorium, bed nucleus of stria terminalis, and a spino-thalamic tract, where it coexisted with CCK-like immunoreactivity as well. No coexistence with CCK occurred in C(i3)-α-peptide-positive ependymal cells outlining the aqueduct and ventricles. Preadsorption of G(i3)-α antibodies with CCK-8 or CCK-33 did not alter G(i3)-α-peptide staining. The occurrence of G(i3)-α-peptide-like immunoreactivity in CCK-containing neurons may indicate the presence of G(i3)-α protein and in CCK/dopamine neurons may indicate an association of this G(i) protein with dopamine autoreceptors.

Original languageEnglish (US)
Pages (from-to)9351-9355
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume85
Issue number23
StatePublished - 1988
Externally publishedYes

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Guanine Nucleotides
Cholecystokinin
Tyrosine 3-Monooxygenase
Immune Sera
Carrier Proteins
Neurons
Brain
Peptides
Staining and Labeling
Autoreceptors
Septal Nuclei
Ventral Tegmental Area
Proteins
Antibodies
Dopaminergic Neurons
Nucleus Accumbens
Colchicine
Substantia Nigra
Indirect Fluorescent Antibody Technique
Hippocampus

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

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title = "Antiserum raised against residues 159-168 of the guanine nucleotide-binding protein G(i3)-α reacts with ependymal cells and some neurons in the rat brain containing cholecystokinin- or cholecystokinin- and tyrosine 3-hydroxylase-like immunoreactivities",
abstract = "Antibodies raised against a synthetic decapeptide corresponding to a specific sequence of G(i3)-α protein (an inhibitory guanine nucleotide-binding protein) were used to analyze G(i3)-α-like immunoreactivity in brain sections from colchicine-treated rats by indirect immunofluorescence histochemistry. G(i3)-α-peptide-positive cell bodies were found in the ventral tegmental area and substantia nigra, and these cells were also cholecystokinin (CCK)- and tyrosine 3-hydroxylase-positive. G(i3)-α-peptide staining was observed in perikarya in the hippocampus and in fibers in the nucleus accumbens, tuberculum olfactorium, bed nucleus of stria terminalis, and a spino-thalamic tract, where it coexisted with CCK-like immunoreactivity as well. No coexistence with CCK occurred in C(i3)-α-peptide-positive ependymal cells outlining the aqueduct and ventricles. Preadsorption of G(i3)-α antibodies with CCK-8 or CCK-33 did not alter G(i3)-α-peptide staining. The occurrence of G(i3)-α-peptide-like immunoreactivity in CCK-containing neurons may indicate the presence of G(i3)-α protein and in CCK/dopamine neurons may indicate an association of this G(i) protein with dopamine autoreceptors.",
author = "R. Cortes and T. Hokfelt and M. Schalling and M. Goldstein and P. Goldsmith and Spiegel, {Allen M.} and C. Unson and J. Walsh",
year = "1988",
language = "English (US)",
volume = "85",
pages = "9351--9355",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "23",

}

TY - JOUR

T1 - Antiserum raised against residues 159-168 of the guanine nucleotide-binding protein G(i3)-α reacts with ependymal cells and some neurons in the rat brain containing cholecystokinin- or cholecystokinin- and tyrosine 3-hydroxylase-like immunoreactivities

AU - Cortes, R.

AU - Hokfelt, T.

AU - Schalling, M.

AU - Goldstein, M.

AU - Goldsmith, P.

AU - Spiegel, Allen M.

AU - Unson, C.

AU - Walsh, J.

PY - 1988

Y1 - 1988

N2 - Antibodies raised against a synthetic decapeptide corresponding to a specific sequence of G(i3)-α protein (an inhibitory guanine nucleotide-binding protein) were used to analyze G(i3)-α-like immunoreactivity in brain sections from colchicine-treated rats by indirect immunofluorescence histochemistry. G(i3)-α-peptide-positive cell bodies were found in the ventral tegmental area and substantia nigra, and these cells were also cholecystokinin (CCK)- and tyrosine 3-hydroxylase-positive. G(i3)-α-peptide staining was observed in perikarya in the hippocampus and in fibers in the nucleus accumbens, tuberculum olfactorium, bed nucleus of stria terminalis, and a spino-thalamic tract, where it coexisted with CCK-like immunoreactivity as well. No coexistence with CCK occurred in C(i3)-α-peptide-positive ependymal cells outlining the aqueduct and ventricles. Preadsorption of G(i3)-α antibodies with CCK-8 or CCK-33 did not alter G(i3)-α-peptide staining. The occurrence of G(i3)-α-peptide-like immunoreactivity in CCK-containing neurons may indicate the presence of G(i3)-α protein and in CCK/dopamine neurons may indicate an association of this G(i) protein with dopamine autoreceptors.

AB - Antibodies raised against a synthetic decapeptide corresponding to a specific sequence of G(i3)-α protein (an inhibitory guanine nucleotide-binding protein) were used to analyze G(i3)-α-like immunoreactivity in brain sections from colchicine-treated rats by indirect immunofluorescence histochemistry. G(i3)-α-peptide-positive cell bodies were found in the ventral tegmental area and substantia nigra, and these cells were also cholecystokinin (CCK)- and tyrosine 3-hydroxylase-positive. G(i3)-α-peptide staining was observed in perikarya in the hippocampus and in fibers in the nucleus accumbens, tuberculum olfactorium, bed nucleus of stria terminalis, and a spino-thalamic tract, where it coexisted with CCK-like immunoreactivity as well. No coexistence with CCK occurred in C(i3)-α-peptide-positive ependymal cells outlining the aqueduct and ventricles. Preadsorption of G(i3)-α antibodies with CCK-8 or CCK-33 did not alter G(i3)-α-peptide staining. The occurrence of G(i3)-α-peptide-like immunoreactivity in CCK-containing neurons may indicate the presence of G(i3)-α protein and in CCK/dopamine neurons may indicate an association of this G(i) protein with dopamine autoreceptors.

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