Antigen-binding mutants of mouse myeloma cells.

W. D. Cook, M. D. Scharff

Research output: Contribution to journalArticle

41 Scopus citations

Abstract

A cultured mouse myeloma cell line, S107, that secretes an IgA phosphocholine-binding immunoglobulin has been cloned in soft agar and overlaid with phosphocholine-hemocyanin. Spontaneous mutants that secrete immunoglobulin with a decreased ability to precipitate antigen were detected with this plate assay and occur at a very high frequency. From one such mutant, phenotypic revertants arise spontaneously with a frequency of 0.28-2.8%. This mutant and one of its revertants were studied, and they were found to differ from the parent and from each other serologically and in antigen binding. While it is not yet clear whether these findings bear any relationship to the normal generation of antibody diversity, they do indicate that it is possible to generate antigen binding diversity in somatic cells.

Original languageEnglish (US)
Pages (from-to)5687-5691
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume74
Issue number12
DOIs
StatePublished - Dec 1977

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Antigen-binding mutants of mouse myeloma cells.'. Together they form a unique fingerprint.

  • Cite this