Antibodies from patients with connective tissue diseases bind specific subsets of cellular RNA-protein particles

J. A. Hardin, D. R. Rahn, C. Shen, M. R. Lerner, S. L. Wolin, M. D. Rosa, J. A. Steitz

Research output: Contribution to journalArticle

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Abstract

We characterized the RNA-containing antigens precipitated by sera from 260 patients with positive antinuclear antibodies. 49 individuals, most of whom had systemic lupus erythematosus or Sjogren's syndrome, possessed antibodies that precipitated the previously identified RNP, Sm, Ro, and La antigens either singly or in combinations. These antigens, which are located on discrete sets of small nuclear or cytoplasmic RNA-protein particles, exhibited a number of antigenic interrelationships. One patient's serum recognized a new particle containing a small RNA which we have called Th; it also precipitated the Ro complexes. Other patients with systemic lupus erythematosus, hepatitis B virus infection, juvenile rheumatoid arthritis, myositis, and rheumatoid arthritis had antibodies that precipitated specific subsets of ribosomal RNA and transfer RNA. One patient's serum contained a monoclonal immunoglobulin G that precipitated ribosomes. Most of these antibodies identified antigenic determinants constituted at least in part of protein. The specificity of the proteins bound to particular cellular RNA, probably explains the exquisite precision with which antibodies from rheumatic disease patients discriminate among RNA subsets. Such sera should be useful probes for investigating specific roles that different RNA and RNA-protein complexes play in cellular metabolism.

Original languageEnglish (US)
Pages (from-to)141-147
Number of pages7
JournalJournal of Clinical Investigation
Volume70
Issue number1
StatePublished - 1982
Externally publishedYes

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Connective Tissue Diseases
RNA
Antibodies
Proteins
Serum
Systemic Lupus Erythematosus
Small Cytoplasmic RNA
Small Nuclear RNA
Antigens
Ribosomal RNA
Juvenile Arthritis
Fibromyalgia
Antinuclear Antibodies
Sjogren's Syndrome
Virus Diseases
Transfer RNA
Rheumatic Diseases
Ribosomes
Hepatitis B virus
Epitopes

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Hardin, J. A., Rahn, D. R., Shen, C., Lerner, M. R., Wolin, S. L., Rosa, M. D., & Steitz, J. A. (1982). Antibodies from patients with connective tissue diseases bind specific subsets of cellular RNA-protein particles. Journal of Clinical Investigation, 70(1), 141-147.

Antibodies from patients with connective tissue diseases bind specific subsets of cellular RNA-protein particles. / Hardin, J. A.; Rahn, D. R.; Shen, C.; Lerner, M. R.; Wolin, S. L.; Rosa, M. D.; Steitz, J. A.

In: Journal of Clinical Investigation, Vol. 70, No. 1, 1982, p. 141-147.

Research output: Contribution to journalArticle

Hardin, JA, Rahn, DR, Shen, C, Lerner, MR, Wolin, SL, Rosa, MD & Steitz, JA 1982, 'Antibodies from patients with connective tissue diseases bind specific subsets of cellular RNA-protein particles', Journal of Clinical Investigation, vol. 70, no. 1, pp. 141-147.
Hardin, J. A. ; Rahn, D. R. ; Shen, C. ; Lerner, M. R. ; Wolin, S. L. ; Rosa, M. D. ; Steitz, J. A. / Antibodies from patients with connective tissue diseases bind specific subsets of cellular RNA-protein particles. In: Journal of Clinical Investigation. 1982 ; Vol. 70, No. 1. pp. 141-147.
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