Anti-aging effects of deuterium depletion on Mn-induced toxicity in a C. elegans model

Daiana Silva Ávila, Gábor Somlyai, Ildikó Somlyai, Michael Aschner

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Work with sub-natural levels of deuterium (D) in animals has demonstrated an anti-cancer effect of low D-concentration in water. Our objective was to investigate whether deuterium-depleted water (DDW) can overturn reverse manganese (Mn)-induced reduction in life span, using the Caenorhabditis elegans (C. elegans) as a model system. DDW per se had no effect on worm's life span 48. h after treatment; however, it reversed the Mn-induced decrease in C. elegans life span. Mn reduced DAF-16 levels, a transcription factor strongly associated with life-span regulation. Low D-concentration (90. ppm) restored the Mn-induced changes in DAF-16 to levels indistinguishable from controls, suggesting DDW can regulate the DAF-16 pathway. We further show that insulin-like receptor DAF-2 levels were unaltered by Mn exposure, tAKT levels increased, whilst superoxide dismutase (SOD-3) levels were decreased by Mn. DDW (90. ppm) restored the levels of tAKT and superoxide dismutase (SOD) to control values without changing DAF-2 levels. Treatment of Mn exposed worms with DDW (90. ppm) restored life-span, DAF-16 and SOD-3 levels to control levels, strongly suggesting that low D concentrations can protect against Mn toxic effects.

Original languageEnglish (US)
Pages (from-to)319-324
Number of pages6
JournalToxicology Letters
Volume211
Issue number3
DOIs
StatePublished - Jun 20 2012
Externally publishedYes

Keywords

  • C. elegans
  • DAF-16
  • DDW
  • Deuterium depletion
  • Life-span
  • Manganese

ASJC Scopus subject areas

  • Toxicology

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