Angiographic, hemodynamic and histological characterization of an arteriovenous fistula in rats

Reza Yassari, T. Sayama, B. S. Jahromi, Y. Aihara, M. Stoodley, R. Loch Macdonald, Michael K. Morgan

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Background. Our understanding of the pathogenesis of arteriovenous malformations (AVMs) and arteriovenous fistulas (AVFs) has been limited by the lack of adequate animal models. In this study we evaluate the time course of angiographic, hemodynamic and histopathological changes in an arteriovenous fistula in rats as a potential model. Methods. An arteriovenous fistula was created by a side-to-end anastomosis of the common carotid artery (CCA) to the external jugular vein (EJV). The animals underwent angiography of the fistula and were sacrificed 1, 7, 21, 42 or 90 days later. Flow and pressure measurements were performed in the CCA and ipsi- and contralateral EJV and detailed histological examination of whole mount sections of the fistula and cranium were done on fixed sections. Immunohistochemistry for CD31, smooth muscle α-actin and Ki-67 were performed. Findings. Hemodynamic changes occur immediately after fistula formation creating a stable high flow, low resistant state. This induces a gradual increase in the inner diameter of the EJV and transverse sinus followed by a decrease in size of the transverse sinus. This decrease is associated with increased expression of α-actin in the wall of the sinus. The fistula becomes angiographically and histologically stable after 21 days. Conclusion. This model describes the time course of hemodynamic and histopathological changes after occur after AVF formation. Stabilization after 21 days makes it an attractive model for mechanistic and therapeutic studies of AVFs.

Original languageEnglish (US)
Pages (from-to)495-504
Number of pages10
JournalActa Neurochirurgica
Volume146
Issue number5
DOIs
StatePublished - May 2004
Externally publishedYes

Fingerprint

Arteriovenous Fistula
Hemodynamics
Fistula
Jugular Veins
Transverse Sinuses
Common Carotid Artery
Actins
Arteriovenous Malformations
Skull
Smooth Muscle
Angiography
Animal Models
Immunohistochemistry
Pressure

Keywords

  • Arteriovenous fistula
  • Arteriovenous malformation
  • Hemodynamics
  • Rat

ASJC Scopus subject areas

  • Clinical Neurology
  • Surgery

Cite this

Yassari, R., Sayama, T., Jahromi, B. S., Aihara, Y., Stoodley, M., Macdonald, R. L., & Morgan, M. K. (2004). Angiographic, hemodynamic and histological characterization of an arteriovenous fistula in rats. Acta Neurochirurgica, 146(5), 495-504. https://doi.org/10.1007/s00701-004-0248-x

Angiographic, hemodynamic and histological characterization of an arteriovenous fistula in rats. / Yassari, Reza; Sayama, T.; Jahromi, B. S.; Aihara, Y.; Stoodley, M.; Macdonald, R. Loch; Morgan, Michael K.

In: Acta Neurochirurgica, Vol. 146, No. 5, 05.2004, p. 495-504.

Research output: Contribution to journalArticle

Yassari, R, Sayama, T, Jahromi, BS, Aihara, Y, Stoodley, M, Macdonald, RL & Morgan, MK 2004, 'Angiographic, hemodynamic and histological characterization of an arteriovenous fistula in rats', Acta Neurochirurgica, vol. 146, no. 5, pp. 495-504. https://doi.org/10.1007/s00701-004-0248-x
Yassari, Reza ; Sayama, T. ; Jahromi, B. S. ; Aihara, Y. ; Stoodley, M. ; Macdonald, R. Loch ; Morgan, Michael K. / Angiographic, hemodynamic and histological characterization of an arteriovenous fistula in rats. In: Acta Neurochirurgica. 2004 ; Vol. 146, No. 5. pp. 495-504.
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