Anesthetic inhibition in ischemic and nonischemic murine heart

Comparison with conscious echocardiographic approach

Shin Takuma, Kotaro Suehiro, Carol Cardinale, Takeshi Hozumi, Hideaki Yano, Juichiro Shimizu, Samantha M. Jansson, Robert Sciacca, Jie Wang, Daniel Burkhoff, Marco R. Di Tullio, Shunichi Homma

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

It is well known that the level of anesthesia obtained by intraperitoneal injection is variable and may alter cardiac function. In this study, we compared the effects of different anesthetics on cardiac function with the conscious state using high-resolution two-dimensional echocardiography in nonischemic and ischemic mice. Eighty-four mice were tested before and after surgery with ligation of the coronary artery. All 84 mice were studied in the conscious state and under high-dose intraperitoneal anesthesia. Twenty-two of 84 mice were studied under low-dose intraperitoneal anesthesia. Another 22 mice were also studied under gas anesthesia and spontaneous breathing. Experiments in the conscious state were performed by two investigators before the administration of anesthesia: one investigator held the animal and the transducer and the other operated the ultrasound equipment. Left ventricular systolic function was measured, and measurements obtained after surgery were compared with infarcted areas assessed by histological staining. Results showed that both high- and low-dose intraperitoneal anesthesia significantly reduced heart rates and left ventricular contractility in both pre- and postsurgical mice as opposed to conscious mice (P < 0.01). There were significantly higher correlation coefficients between mean fractional area change (FAC) and infarcted area in conscious state compared with high-dose intraperitoneal anesthesia (P < 0.05). The correlation coefficient between FAC and infarcted area during gas anesthesia was also significantly higher compared with high-dose intraperitoneal anesthesia (P < 0.05). In conclusion, conscious experiments or the use of gas anesthesia is preferred for echocardiographic assessment of cardiac function in mice because intraperitoneal injection significantly induces a significant reduction in heart rate and left ventricular systolic function.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume280
Issue number5 49-5
StatePublished - May 2001
Externally publishedYes

Fingerprint

Anesthetics
Anesthesia
Gases
Intraperitoneal Injections
Left Ventricular Function
Heart Rate
Research Personnel
Transducers
Ligation
Echocardiography
Coronary Vessels
Respiration
Staining and Labeling
Equipment and Supplies

Keywords

  • Cardiac function
  • Intraperitoneal
  • M-mode
  • Noninvasive

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Anesthetic inhibition in ischemic and nonischemic murine heart : Comparison with conscious echocardiographic approach. / Takuma, Shin; Suehiro, Kotaro; Cardinale, Carol; Hozumi, Takeshi; Yano, Hideaki; Shimizu, Juichiro; Jansson, Samantha M.; Sciacca, Robert; Wang, Jie; Burkhoff, Daniel; Di Tullio, Marco R.; Homma, Shunichi.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 280, No. 5 49-5, 05.2001.

Research output: Contribution to journalArticle

Takuma, S, Suehiro, K, Cardinale, C, Hozumi, T, Yano, H, Shimizu, J, Jansson, SM, Sciacca, R, Wang, J, Burkhoff, D, Di Tullio, MR & Homma, S 2001, 'Anesthetic inhibition in ischemic and nonischemic murine heart: Comparison with conscious echocardiographic approach', American Journal of Physiology - Heart and Circulatory Physiology, vol. 280, no. 5 49-5.
Takuma, Shin ; Suehiro, Kotaro ; Cardinale, Carol ; Hozumi, Takeshi ; Yano, Hideaki ; Shimizu, Juichiro ; Jansson, Samantha M. ; Sciacca, Robert ; Wang, Jie ; Burkhoff, Daniel ; Di Tullio, Marco R. ; Homma, Shunichi. / Anesthetic inhibition in ischemic and nonischemic murine heart : Comparison with conscious echocardiographic approach. In: American Journal of Physiology - Heart and Circulatory Physiology. 2001 ; Vol. 280, No. 5 49-5.
@article{054b7da437204cc5994b0a0c4fa760d8,
title = "Anesthetic inhibition in ischemic and nonischemic murine heart: Comparison with conscious echocardiographic approach",
abstract = "It is well known that the level of anesthesia obtained by intraperitoneal injection is variable and may alter cardiac function. In this study, we compared the effects of different anesthetics on cardiac function with the conscious state using high-resolution two-dimensional echocardiography in nonischemic and ischemic mice. Eighty-four mice were tested before and after surgery with ligation of the coronary artery. All 84 mice were studied in the conscious state and under high-dose intraperitoneal anesthesia. Twenty-two of 84 mice were studied under low-dose intraperitoneal anesthesia. Another 22 mice were also studied under gas anesthesia and spontaneous breathing. Experiments in the conscious state were performed by two investigators before the administration of anesthesia: one investigator held the animal and the transducer and the other operated the ultrasound equipment. Left ventricular systolic function was measured, and measurements obtained after surgery were compared with infarcted areas assessed by histological staining. Results showed that both high- and low-dose intraperitoneal anesthesia significantly reduced heart rates and left ventricular contractility in both pre- and postsurgical mice as opposed to conscious mice (P < 0.01). There were significantly higher correlation coefficients between mean fractional area change (FAC) and infarcted area in conscious state compared with high-dose intraperitoneal anesthesia (P < 0.05). The correlation coefficient between FAC and infarcted area during gas anesthesia was also significantly higher compared with high-dose intraperitoneal anesthesia (P < 0.05). In conclusion, conscious experiments or the use of gas anesthesia is preferred for echocardiographic assessment of cardiac function in mice because intraperitoneal injection significantly induces a significant reduction in heart rate and left ventricular systolic function.",
keywords = "Cardiac function, Intraperitoneal, M-mode, Noninvasive",
author = "Shin Takuma and Kotaro Suehiro and Carol Cardinale and Takeshi Hozumi and Hideaki Yano and Juichiro Shimizu and Jansson, {Samantha M.} and Robert Sciacca and Jie Wang and Daniel Burkhoff and {Di Tullio}, {Marco R.} and Shunichi Homma",
year = "2001",
month = "5",
language = "English (US)",
volume = "280",
journal = "American Journal of Physiology - Renal Fluid and Electrolyte Physiology",
issn = "1931-857X",
publisher = "American Physiological Society",
number = "5 49-5",

}

TY - JOUR

T1 - Anesthetic inhibition in ischemic and nonischemic murine heart

T2 - Comparison with conscious echocardiographic approach

AU - Takuma, Shin

AU - Suehiro, Kotaro

AU - Cardinale, Carol

AU - Hozumi, Takeshi

AU - Yano, Hideaki

AU - Shimizu, Juichiro

AU - Jansson, Samantha M.

AU - Sciacca, Robert

AU - Wang, Jie

AU - Burkhoff, Daniel

AU - Di Tullio, Marco R.

AU - Homma, Shunichi

PY - 2001/5

Y1 - 2001/5

N2 - It is well known that the level of anesthesia obtained by intraperitoneal injection is variable and may alter cardiac function. In this study, we compared the effects of different anesthetics on cardiac function with the conscious state using high-resolution two-dimensional echocardiography in nonischemic and ischemic mice. Eighty-four mice were tested before and after surgery with ligation of the coronary artery. All 84 mice were studied in the conscious state and under high-dose intraperitoneal anesthesia. Twenty-two of 84 mice were studied under low-dose intraperitoneal anesthesia. Another 22 mice were also studied under gas anesthesia and spontaneous breathing. Experiments in the conscious state were performed by two investigators before the administration of anesthesia: one investigator held the animal and the transducer and the other operated the ultrasound equipment. Left ventricular systolic function was measured, and measurements obtained after surgery were compared with infarcted areas assessed by histological staining. Results showed that both high- and low-dose intraperitoneal anesthesia significantly reduced heart rates and left ventricular contractility in both pre- and postsurgical mice as opposed to conscious mice (P < 0.01). There were significantly higher correlation coefficients between mean fractional area change (FAC) and infarcted area in conscious state compared with high-dose intraperitoneal anesthesia (P < 0.05). The correlation coefficient between FAC and infarcted area during gas anesthesia was also significantly higher compared with high-dose intraperitoneal anesthesia (P < 0.05). In conclusion, conscious experiments or the use of gas anesthesia is preferred for echocardiographic assessment of cardiac function in mice because intraperitoneal injection significantly induces a significant reduction in heart rate and left ventricular systolic function.

AB - It is well known that the level of anesthesia obtained by intraperitoneal injection is variable and may alter cardiac function. In this study, we compared the effects of different anesthetics on cardiac function with the conscious state using high-resolution two-dimensional echocardiography in nonischemic and ischemic mice. Eighty-four mice were tested before and after surgery with ligation of the coronary artery. All 84 mice were studied in the conscious state and under high-dose intraperitoneal anesthesia. Twenty-two of 84 mice were studied under low-dose intraperitoneal anesthesia. Another 22 mice were also studied under gas anesthesia and spontaneous breathing. Experiments in the conscious state were performed by two investigators before the administration of anesthesia: one investigator held the animal and the transducer and the other operated the ultrasound equipment. Left ventricular systolic function was measured, and measurements obtained after surgery were compared with infarcted areas assessed by histological staining. Results showed that both high- and low-dose intraperitoneal anesthesia significantly reduced heart rates and left ventricular contractility in both pre- and postsurgical mice as opposed to conscious mice (P < 0.01). There were significantly higher correlation coefficients between mean fractional area change (FAC) and infarcted area in conscious state compared with high-dose intraperitoneal anesthesia (P < 0.05). The correlation coefficient between FAC and infarcted area during gas anesthesia was also significantly higher compared with high-dose intraperitoneal anesthesia (P < 0.05). In conclusion, conscious experiments or the use of gas anesthesia is preferred for echocardiographic assessment of cardiac function in mice because intraperitoneal injection significantly induces a significant reduction in heart rate and left ventricular systolic function.

KW - Cardiac function

KW - Intraperitoneal

KW - M-mode

KW - Noninvasive

UR - http://www.scopus.com/inward/record.url?scp=0035030886&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035030886&partnerID=8YFLogxK

M3 - Article

VL - 280

JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

SN - 1931-857X

IS - 5 49-5

ER -