Analyzing mrna expression using single mrna resolution fluorescent in situ hybridization

Daniel Zenklusen, Robert H. Singer

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

As the product of transcription and the blueprint for translation, mRNA is the main Intermediate product of the gene expression pathway. The ability to accurately determine mRNA levels is, therefore, a major requirement when studying gene expression. mRNA is also a target of different regulatory steps, occurring in different subcellular compartments. To understand the different

Original languageEnglish (US)
Pages (from-to)641-659
Number of pages19
JournalMethods in Enzymology
Volume470
Issue numberC
DOIs
StatePublished - 2010

Fingerprint

Fluorescence In Situ Hybridization
Gene Expression
Gene expression
Messenger RNA
Protein Biosynthesis
Blueprints
Transcription

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Analyzing mrna expression using single mrna resolution fluorescent in situ hybridization. / Zenklusen, Daniel; Singer, Robert H.

In: Methods in Enzymology, Vol. 470, No. C, 2010, p. 641-659.

Research output: Contribution to journalArticle

@article{e8b2f2596e5e453b86593ac891bb9318,
title = "Analyzing mrna expression using single mrna resolution fluorescent in situ hybridization",
abstract = "As the product of transcription and the blueprint for translation, mRNA is the main Intermediate product of the gene expression pathway. The ability to accurately determine mRNA levels is, therefore, a major requirement when studying gene expression. mRNA is also a target of different regulatory steps, occurring in different subcellular compartments. To understand the different",
author = "Daniel Zenklusen and Singer, {Robert H.}",
year = "2010",
doi = "10.1016/S0076-6879(10)70026-4",
language = "English (US)",
volume = "470",
pages = "641--659",
journal = "ImmunoMethods",
issn = "1046-2023",
publisher = "Academic Press Inc.",
number = "C",

}

TY - JOUR

T1 - Analyzing mrna expression using single mrna resolution fluorescent in situ hybridization

AU - Zenklusen, Daniel

AU - Singer, Robert H.

PY - 2010

Y1 - 2010

N2 - As the product of transcription and the blueprint for translation, mRNA is the main Intermediate product of the gene expression pathway. The ability to accurately determine mRNA levels is, therefore, a major requirement when studying gene expression. mRNA is also a target of different regulatory steps, occurring in different subcellular compartments. To understand the different

AB - As the product of transcription and the blueprint for translation, mRNA is the main Intermediate product of the gene expression pathway. The ability to accurately determine mRNA levels is, therefore, a major requirement when studying gene expression. mRNA is also a target of different regulatory steps, occurring in different subcellular compartments. To understand the different

UR - http://www.scopus.com/inward/record.url?scp=77956027418&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77956027418&partnerID=8YFLogxK

U2 - 10.1016/S0076-6879(10)70026-4

DO - 10.1016/S0076-6879(10)70026-4

M3 - Article

C2 - 20946829

AN - SCOPUS:77956027418

VL - 470

SP - 641

EP - 659

JO - ImmunoMethods

JF - ImmunoMethods

SN - 1046-2023

IS - C

ER -