Analyzing mrna expression using single mrna resolution fluorescent in situ hybridization

Daniel Zenklusen; Robert H. Singer

doi:10.1016/S0076-6879(10)70026-4

Analyzing mrna expression using single mrna resolution fluorescent in situ hybridization

Daniel Zenklusen, Robert H. Singer

Cell Biology

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

As the product of transcription and the blueprint for translation, mRNA is the main Intermediate product of the gene expression pathway. The ability to accurately determine mRNA levels is, therefore, a major requirement when studying gene expression. mRNA is also a target of different regulatory steps, occurring in different subcellular compartments. To understand the different

Original language	English (US)
Pages (from-to)	641-659
Number of pages	19
Journal	Methods in enzymology
Volume	470
Issue number	C
DOIs	https://doi.org/10.1016/S0076-6879(10)70026-4
State	Published - 2010

ASJC Scopus subject areas

Biochemistry
Molecular Biology

Access to Document

10.1016/S0076-6879(10)70026-4

Cite this

@article{e8b2f2596e5e453b86593ac891bb9318,

title = "Analyzing mrna expression using single mrna resolution fluorescent in situ hybridization",

abstract = "As the product of transcription and the blueprint for translation, mRNA is the main Intermediate product of the gene expression pathway. The ability to accurately determine mRNA levels is, therefore, a major requirement when studying gene expression. mRNA is also a target of different regulatory steps, occurring in different subcellular compartments. To understand the different",

author = "Daniel Zenklusen and Singer, {Robert H.}",

note = "Funding Information: We thank S. Hocine, S. Gandhi, and M. Oeffinger for suggestions and critical reading of the manuscript. This work was supported by NIH grant GM57071 to R. H. S.",

year = "2010",

doi = "10.1016/S0076-6879(10)70026-4",

language = "English (US)",

volume = "470",

pages = "641--659",

journal = "Methods in enzymology",

issn = "0076-6879",

publisher = "Academic Press Inc.",

number = "C",

}

TY - JOUR

T1 - Analyzing mrna expression using single mrna resolution fluorescent in situ hybridization

AU - Zenklusen, Daniel

AU - Singer, Robert H.

N1 - Funding Information: We thank S. Hocine, S. Gandhi, and M. Oeffinger for suggestions and critical reading of the manuscript. This work was supported by NIH grant GM57071 to R. H. S.

PY - 2010

Y1 - 2010

N2 - As the product of transcription and the blueprint for translation, mRNA is the main Intermediate product of the gene expression pathway. The ability to accurately determine mRNA levels is, therefore, a major requirement when studying gene expression. mRNA is also a target of different regulatory steps, occurring in different subcellular compartments. To understand the different

AB - As the product of transcription and the blueprint for translation, mRNA is the main Intermediate product of the gene expression pathway. The ability to accurately determine mRNA levels is, therefore, a major requirement when studying gene expression. mRNA is also a target of different regulatory steps, occurring in different subcellular compartments. To understand the different

UR - http://www.scopus.com/inward/record.url?scp=77956027418&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77956027418&partnerID=8YFLogxK

U2 - 10.1016/S0076-6879(10)70026-4

DO - 10.1016/S0076-6879(10)70026-4

M3 - Article

C2 - 20946829

AN - SCOPUS:77956027418

SN - 0076-6879

VL - 470

SP - 641

EP - 659

JO - Methods in enzymology

JF - Methods in enzymology

IS - C

ER -

Analyzing mrna expression using single mrna resolution fluorescent in situ hybridization

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this