Analyzing mrna expression using single mrna resolution fluorescent in situ hybridization

Daniel Zenklusen, Robert H. Singer

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

As the product of transcription and the blueprint for translation, mRNA is the main Intermediate product of the gene expression pathway. The ability to accurately determine mRNA levels is, therefore, a major requirement when studying gene expression. mRNA is also a target of different regulatory steps, occurring in different subcellular compartments. To understand the different

Original languageEnglish (US)
Pages (from-to)641-659
Number of pages19
JournalMethods in enzymology
Volume470
Issue numberC
DOIs
StatePublished - 2010

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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