Analysis of the significance of two single-base-pair differences in the SL3-3 Akv virus long terminal repeats

Harry L. Morrison, Hong Yan Dai, Finn Skou Pedersen, Jack Lenz

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Two single-base-pair differences between the long terminal repeats (LTRs) of the T-lymphomagenic murine retrovirus SL3-3 and nonleukemogenic Akv virus were tested for effects on activity of the LTRs, Evidence was obtained from electrophoretic mobility shift assays for the presence of at least one factor in both T and non-T cells that bound to the region of the viral enhancers that contained the differences. However, no significant differences in activity in expression assays were detected when the two base-pair differences were exchanged between the two LTRs. Therefore, they do not contribute to the higher activity of the SL3-3 LTR in T-lymphoma cell lines.

Original languageEnglish (US)
Pages (from-to)1019-1022
Number of pages4
JournalJournal of Virology
Volume65
Issue number2
StatePublished - 1991

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terminal repeat sequences
Terminal Repeat Sequences
Base Pairing
Viruses
viruses
Retroviridae
Electrophoretic Mobility Shift Assay
assays
lymphoma
Lymphoma
cell lines
Cell Line
mice
cells

ASJC Scopus subject areas

  • Immunology

Cite this

Analysis of the significance of two single-base-pair differences in the SL3-3 Akv virus long terminal repeats. / Morrison, Harry L.; Dai, Hong Yan; Pedersen, Finn Skou; Lenz, Jack.

In: Journal of Virology, Vol. 65, No. 2, 1991, p. 1019-1022.

Research output: Contribution to journalArticle

Morrison, Harry L. ; Dai, Hong Yan ; Pedersen, Finn Skou ; Lenz, Jack. / Analysis of the significance of two single-base-pair differences in the SL3-3 Akv virus long terminal repeats. In: Journal of Virology. 1991 ; Vol. 65, No. 2. pp. 1019-1022.
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