Analysis of the hotfoot (ho) locus by creation of an insertional mutation in a transgenic mouse

Jon W. Gordon, Joan Uehlinger, Nooshine Dayani, Beth E. Talansky, Mildred Gordon, Greg S. Rudomen, Paul E. Neumann

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Hotfoot (ho) mutation is a recessive trait in mice, characterized by motor disorder and male sterility, that maps to chromosome 6. We have identified a transgenic mouse pedigree with a similar trait. Using genetic and molecular approaches, we have demonstrated that the foreign DNA element is located in or near the ho locus. This new allele, designated hoJwg and presumably created by insertional mutagenesis, should make it possible to clone the ho gene. Male infertility in hoJwg male homozygotes was determined to be due to inability of sperm to penetrate the zona pellucida. This was demonstrated by rescuing mutant males by a new technique of gamete micromanipulation, zona pellucida drilling. These findings show that zona drilling is useful both for analysis and preservation of animals with reduced male fertility.

Original languageEnglish (US)
Pages (from-to)349-358
Number of pages10
JournalDevelopmental Biology
Volume137
Issue number2
DOIs
StatePublished - 1990
Externally publishedYes

Fingerprint

Transgenic Mice
Zona Pellucida
Male Infertility
Mutation
Micromanipulation
Chromosomes, Human, Pair 6
Insertional Mutagenesis
Herpes Zoster
Homozygote
Pedigree
Germ Cells
Fertility
Spermatozoa
Molecular Biology
Clone Cells
Alleles
DNA
Genes
Motor Disorders

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Gordon, J. W., Uehlinger, J., Dayani, N., Talansky, B. E., Gordon, M., Rudomen, G. S., & Neumann, P. E. (1990). Analysis of the hotfoot (ho) locus by creation of an insertional mutation in a transgenic mouse. Developmental Biology, 137(2), 349-358. https://doi.org/10.1016/0012-1606(90)90259-L

Analysis of the hotfoot (ho) locus by creation of an insertional mutation in a transgenic mouse. / Gordon, Jon W.; Uehlinger, Joan; Dayani, Nooshine; Talansky, Beth E.; Gordon, Mildred; Rudomen, Greg S.; Neumann, Paul E.

In: Developmental Biology, Vol. 137, No. 2, 1990, p. 349-358.

Research output: Contribution to journalArticle

Gordon, JW, Uehlinger, J, Dayani, N, Talansky, BE, Gordon, M, Rudomen, GS & Neumann, PE 1990, 'Analysis of the hotfoot (ho) locus by creation of an insertional mutation in a transgenic mouse', Developmental Biology, vol. 137, no. 2, pp. 349-358. https://doi.org/10.1016/0012-1606(90)90259-L
Gordon, Jon W. ; Uehlinger, Joan ; Dayani, Nooshine ; Talansky, Beth E. ; Gordon, Mildred ; Rudomen, Greg S. ; Neumann, Paul E. / Analysis of the hotfoot (ho) locus by creation of an insertional mutation in a transgenic mouse. In: Developmental Biology. 1990 ; Vol. 137, No. 2. pp. 349-358.
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