Analysis of primary cilia in directional cell migration in fibroblasts

Søren T. Christensen, Iben R. Veland, Albrecht Schwab, Michael Cammer, Peter Satir

Research output: Chapter in Book/Report/Conference proceedingChapter

12 Scopus citations

Abstract

Early studies of migrating fibroblasts showed that primary cilia orient in front of the nucleus and point toward the leading edge. Recent work has shown that primary cilia coordinate a series of signaling pathways critical to fibroblast cell migration during development and in wound healing. In particular, platelet-derived growth factor receptor alpha (PDGFRα) is compartmentalized to the primary cilium to activate signaling pathways that regulate reorganization of the cytoskeleton required for lamellipodium formation and directional migration in the presence of a specific ligand gradient. We summarize selected methods in analyzing ciliary function in directional cell migration, including immunofluorescence microscopy, scratch assay, and chemotaxis assay by micropipette addition of PDGFRα ligands to cultures of fibroblasts. These methods should be useful not only in studying cell migration but also more generally in delineating response pathways in cells with primary cilia.

Original languageEnglish (US)
Title of host publicationCilia, Part B
PublisherAcademic Press Inc.
Pages45-58
Number of pages14
ISBN (Print)9780123979445
DOIs
StatePublished - 2013

Publication series

NameMethods in Enzymology
Volume525
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • Chemotaxis
  • Directional cell migration
  • Fibroblasts
  • PDGF-AA
  • PDGFRα
  • Primary cilia
  • Primary cilium

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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    Christensen, S. T., Veland, I. R., Schwab, A., Cammer, M., & Satir, P. (2013). Analysis of primary cilia in directional cell migration in fibroblasts. In Cilia, Part B (pp. 45-58). (Methods in Enzymology; Vol. 525). Academic Press Inc.. https://doi.org/10.1016/B978-0-12-397944-5.00003-1