Analysis of Integrin Signaling in Genetically Engineered Mouse Models of Mammary Tumor Progression

Yuliya Pylayeva, Wenjun Guo, Filippo G. Giancotti

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Cancer progression-the evolution of malignant tumors towards metastatic dissemination-is a complex, multistep process orchestrated by neoplastic cells but aided by elements of the tumor microenvironment such as macrophages, activated fibroblasts, and endothelial cells. During tumor progression, cancer cells acquire a number of traits, such as the ability to undergo unrestrained proliferation, to resist pro-apoptotic insults, and to invade through tissue boundaries. Genetic and epigenetic changes conspire to drive the emergence of these traits against the backdrop of host selection. It is becoming increasingly clear that certain integrins and integrin-signaling components amplify oncogenic signaling to promote tumor progression. Mouse models of cancer provide useful, if not necessary, experimental systems to study tumor initiation and progression in vivo and to test novel therapeutic approaches. We have utilized mouse models of mammary tumorigenesis to examine the role of integrin α6β4 signaling in tumor progression in vivo. In this chapter, we describe a collection of cell biological and genetic methods that may aid in characterizing the roles of integrin signals in mammary tumorigenesis.

Original languageEnglish (US)
Pages (from-to)439-461
Number of pages23
JournalMethods in Enzymology
Volume426
DOIs
StatePublished - 2007
Externally publishedYes

Fingerprint

Integrins
Tumors
Breast Neoplasms
Neoplasms
Carcinogenesis
Breast
Macrophages
Endothelial cells
Fibroblasts
Neoplastic Processes
Tumor Microenvironment
Cells
Epigenomics
Tissue
Endothelial Cells

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Analysis of Integrin Signaling in Genetically Engineered Mouse Models of Mammary Tumor Progression. / Pylayeva, Yuliya; Guo, Wenjun; Giancotti, Filippo G.

In: Methods in Enzymology, Vol. 426, 2007, p. 439-461.

Research output: Contribution to journalArticle

@article{ef1d57c03e6f42ae98c524d1f71a015f,
title = "Analysis of Integrin Signaling in Genetically Engineered Mouse Models of Mammary Tumor Progression",
abstract = "Cancer progression-the evolution of malignant tumors towards metastatic dissemination-is a complex, multistep process orchestrated by neoplastic cells but aided by elements of the tumor microenvironment such as macrophages, activated fibroblasts, and endothelial cells. During tumor progression, cancer cells acquire a number of traits, such as the ability to undergo unrestrained proliferation, to resist pro-apoptotic insults, and to invade through tissue boundaries. Genetic and epigenetic changes conspire to drive the emergence of these traits against the backdrop of host selection. It is becoming increasingly clear that certain integrins and integrin-signaling components amplify oncogenic signaling to promote tumor progression. Mouse models of cancer provide useful, if not necessary, experimental systems to study tumor initiation and progression in vivo and to test novel therapeutic approaches. We have utilized mouse models of mammary tumorigenesis to examine the role of integrin α6β4 signaling in tumor progression in vivo. In this chapter, we describe a collection of cell biological and genetic methods that may aid in characterizing the roles of integrin signals in mammary tumorigenesis.",
author = "Yuliya Pylayeva and Wenjun Guo and Giancotti, {Filippo G.}",
year = "2007",
doi = "10.1016/S0076-6879(07)26019-7",
language = "English (US)",
volume = "426",
pages = "439--461",
journal = "Methods",
issn = "1046-2023",
publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Analysis of Integrin Signaling in Genetically Engineered Mouse Models of Mammary Tumor Progression

AU - Pylayeva, Yuliya

AU - Guo, Wenjun

AU - Giancotti, Filippo G.

PY - 2007

Y1 - 2007

N2 - Cancer progression-the evolution of malignant tumors towards metastatic dissemination-is a complex, multistep process orchestrated by neoplastic cells but aided by elements of the tumor microenvironment such as macrophages, activated fibroblasts, and endothelial cells. During tumor progression, cancer cells acquire a number of traits, such as the ability to undergo unrestrained proliferation, to resist pro-apoptotic insults, and to invade through tissue boundaries. Genetic and epigenetic changes conspire to drive the emergence of these traits against the backdrop of host selection. It is becoming increasingly clear that certain integrins and integrin-signaling components amplify oncogenic signaling to promote tumor progression. Mouse models of cancer provide useful, if not necessary, experimental systems to study tumor initiation and progression in vivo and to test novel therapeutic approaches. We have utilized mouse models of mammary tumorigenesis to examine the role of integrin α6β4 signaling in tumor progression in vivo. In this chapter, we describe a collection of cell biological and genetic methods that may aid in characterizing the roles of integrin signals in mammary tumorigenesis.

AB - Cancer progression-the evolution of malignant tumors towards metastatic dissemination-is a complex, multistep process orchestrated by neoplastic cells but aided by elements of the tumor microenvironment such as macrophages, activated fibroblasts, and endothelial cells. During tumor progression, cancer cells acquire a number of traits, such as the ability to undergo unrestrained proliferation, to resist pro-apoptotic insults, and to invade through tissue boundaries. Genetic and epigenetic changes conspire to drive the emergence of these traits against the backdrop of host selection. It is becoming increasingly clear that certain integrins and integrin-signaling components amplify oncogenic signaling to promote tumor progression. Mouse models of cancer provide useful, if not necessary, experimental systems to study tumor initiation and progression in vivo and to test novel therapeutic approaches. We have utilized mouse models of mammary tumorigenesis to examine the role of integrin α6β4 signaling in tumor progression in vivo. In this chapter, we describe a collection of cell biological and genetic methods that may aid in characterizing the roles of integrin signals in mammary tumorigenesis.

UR - http://www.scopus.com/inward/record.url?scp=35148889801&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=35148889801&partnerID=8YFLogxK

U2 - 10.1016/S0076-6879(07)26019-7

DO - 10.1016/S0076-6879(07)26019-7

M3 - Article

VL - 426

SP - 439

EP - 461

JO - Methods

JF - Methods

SN - 1046-2023

ER -