Analysis of determinants in filovirus glycoproteins required for tetherin antagonism

Kerstin Gnirß, Marie Fiedler, Annika Krämer-Kühl, Sebastian Bolduan, Eva-Maria Mittler, Stephan Becker, Michael Schindler, Stefan Pöhlmann

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The host cell protein tetherin can restrict the release of enveloped viruses from infected cells. The HIV-1 protein Vpu counteracts tetherin by removing it from the site of viral budding, the plasma membrane, and this process depends on specific interactions between the transmembrane domains of Vpu and tetherin. In contrast, the glycoproteins (GPs) of two filoviruses, Ebola and Marburg virus, antagonize tetherin without reducing surface expression, and the domains in GP required for tetherin counteraction are unknown. Here, we show that filovirus GPs depend on the presence of their authentic transmembrane domains for virus-cell fusion and tetherin antagonism. However, conserved residues within the transmembrane domain were dispensable for membrane fusion and tetherin counteraction. Moreover, the insertion of the transmembrane domain into a heterologous viral GP, Lassa virus GPC, was not sufficient to confer tetherin antagonism to the recipient. Finally, mutation of conserved residues within the fusion peptide of Ebola virus GP inhibited virus-cell fusion but did not ablate tetherin counteraction, indicating that the fusion peptide and the ability of GP to drive host cell entry are not required for tetherin counteraction. These results suggest that the transmembrane domains of filoviral GPs contribute to tetherin antagonism but are not the sole determinants.

Original languageEnglish (US)
Pages (from-to)1654-1671
Number of pages18
JournalViruses
Volume6
Issue number4
DOIs
StatePublished - Apr 9 2014

Fingerprint

Glycoproteins
Ebolavirus
Cell Fusion
Lassa virus
Marburgvirus
Viruses
Human Immunodeficiency Virus Proteins
Virus Release
Peptides
Membrane Fusion
HIV-1
Cell Membrane
Mutation
Proteins

Keywords

  • Ebola
  • Glycoprotein
  • Lassa
  • Tetherin

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology
  • Medicine(all)

Cite this

Gnirß, K., Fiedler, M., Krämer-Kühl, A., Bolduan, S., Mittler, E-M., Becker, S., ... Pöhlmann, S. (2014). Analysis of determinants in filovirus glycoproteins required for tetherin antagonism. Viruses, 6(4), 1654-1671. https://doi.org/10.3390/v6041654

Analysis of determinants in filovirus glycoproteins required for tetherin antagonism. / Gnirß, Kerstin; Fiedler, Marie; Krämer-Kühl, Annika; Bolduan, Sebastian; Mittler, Eva-Maria; Becker, Stephan; Schindler, Michael; Pöhlmann, Stefan.

In: Viruses, Vol. 6, No. 4, 09.04.2014, p. 1654-1671.

Research output: Contribution to journalArticle

Gnirß, K, Fiedler, M, Krämer-Kühl, A, Bolduan, S, Mittler, E-M, Becker, S, Schindler, M & Pöhlmann, S 2014, 'Analysis of determinants in filovirus glycoproteins required for tetherin antagonism', Viruses, vol. 6, no. 4, pp. 1654-1671. https://doi.org/10.3390/v6041654
Gnirß K, Fiedler M, Krämer-Kühl A, Bolduan S, Mittler E-M, Becker S et al. Analysis of determinants in filovirus glycoproteins required for tetherin antagonism. Viruses. 2014 Apr 9;6(4):1654-1671. https://doi.org/10.3390/v6041654
Gnirß, Kerstin ; Fiedler, Marie ; Krämer-Kühl, Annika ; Bolduan, Sebastian ; Mittler, Eva-Maria ; Becker, Stephan ; Schindler, Michael ; Pöhlmann, Stefan. / Analysis of determinants in filovirus glycoproteins required for tetherin antagonism. In: Viruses. 2014 ; Vol. 6, No. 4. pp. 1654-1671.
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