Analysis of chaperone-mediated autophagy

Y. R. Juste, Ana Maria Cuervo

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

Chaperone-mediated autophagy (CMA) is a selective type of autophagy whereby a specific subset of intracellular proteins is targeted to the lysosome for degradation. These proteins are identified by a chaperone that targets them to lysosomes. There, they are translocated into the organelle lumen through a lysosomal membrane receptor/translocation complex. CMA plays an important role in maintaining cellular proteostasis by eliminating damaged and altered proteins. CMA also participates in the control of the cellular energetic balance through recycling of amino acids resulting from lysosomal proteolysis of the substrate proteins. Lastly, due to the intrinsic protein selectivity of CMA, this type of autophagy exerts regulatory functions by mediating timely degradation of key cellular proteins that participate in processes such as lipid and glucose metabolism, cell cycle, DNA repair, and cellular reprogramming, among others. Dysfunctional CMA occurs with age and has now been described in a growing list of human pathologies such as metabolic disorders, neurodegeneration, cancer, immunodeficiency, and diabetes. In this chapter, we describe current methodologies to quantitatively analyze CMA activity in different experimental models.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages703-727
Number of pages25
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1880
ISSN (Print)1064-3745

Fingerprint

Autophagy
Proteins
Lysosomes
Recycling
Lipid Metabolism
DNA Repair
Organelles
Proteolysis
Cell Cycle
Theoretical Models
Pathology
Amino Acids
Glucose
Membranes

Keywords

  • Chaperones
  • Lysosomes
  • Proteolysis
  • Subcellular fractionation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Juste, Y. R., & Cuervo, A. M. (2019). Analysis of chaperone-mediated autophagy. In Methods in Molecular Biology (pp. 703-727). (Methods in Molecular Biology; Vol. 1880). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8873-0_47

Analysis of chaperone-mediated autophagy. / Juste, Y. R.; Cuervo, Ana Maria.

Methods in Molecular Biology. Humana Press Inc., 2019. p. 703-727 (Methods in Molecular Biology; Vol. 1880).

Research output: Chapter in Book/Report/Conference proceedingChapter

Juste, YR & Cuervo, AM 2019, Analysis of chaperone-mediated autophagy. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1880, Humana Press Inc., pp. 703-727. https://doi.org/10.1007/978-1-4939-8873-0_47
Juste YR, Cuervo AM. Analysis of chaperone-mediated autophagy. In Methods in Molecular Biology. Humana Press Inc. 2019. p. 703-727. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-8873-0_47
Juste, Y. R. ; Cuervo, Ana Maria. / Analysis of chaperone-mediated autophagy. Methods in Molecular Biology. Humana Press Inc., 2019. pp. 703-727 (Methods in Molecular Biology).
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