Abstract
Many aspects of Drosophila eye development depend on receptor tyrosine kinases that signal through Ras. Genetic studies and genetic screens using eye morphology and development as assays have identified major components of receptor tyrosine kinase and Ras signaling and outlined specific contributions of these components to cell fate specification and differentiation, cell survival, cell cycle progression and arrest, and cellular movements and morphology. This chapter presents a brief compendium of methods and strains that may be used to obtain overexpression or loss of function for Ras pathway genes in the eye and methods and reagents permitting initial characterization of retinal cell differentiation, death, and cell cycle behavior.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 711-721 |
| Number of pages | 11 |
| Journal | Methods in Enzymology |
| Volume | 407 |
| DOIs | |
| State | Published - 2005 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Biochemistry
Cite this
Analyses of RAS Regulation of Eye Development in Drosophila melanogaster. / Firth, Lucy C.; Li, Wei; Zhang, Hui; Baker, Nicholas E.
In: Methods in Enzymology, Vol. 407, 2005, p. 711-721.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Analyses of RAS Regulation of Eye Development in Drosophila melanogaster
AU - Firth, Lucy C.
AU - Li, Wei
AU - Zhang, Hui
AU - Baker, Nicholas E.
PY - 2005
Y1 - 2005
N2 - Many aspects of Drosophila eye development depend on receptor tyrosine kinases that signal through Ras. Genetic studies and genetic screens using eye morphology and development as assays have identified major components of receptor tyrosine kinase and Ras signaling and outlined specific contributions of these components to cell fate specification and differentiation, cell survival, cell cycle progression and arrest, and cellular movements and morphology. This chapter presents a brief compendium of methods and strains that may be used to obtain overexpression or loss of function for Ras pathway genes in the eye and methods and reagents permitting initial characterization of retinal cell differentiation, death, and cell cycle behavior.
AB - Many aspects of Drosophila eye development depend on receptor tyrosine kinases that signal through Ras. Genetic studies and genetic screens using eye morphology and development as assays have identified major components of receptor tyrosine kinase and Ras signaling and outlined specific contributions of these components to cell fate specification and differentiation, cell survival, cell cycle progression and arrest, and cellular movements and morphology. This chapter presents a brief compendium of methods and strains that may be used to obtain overexpression or loss of function for Ras pathway genes in the eye and methods and reagents permitting initial characterization of retinal cell differentiation, death, and cell cycle behavior.
UR - http://www.scopus.com/inward/record.url?scp=33744509562&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33744509562&partnerID=8YFLogxK
U2 - 10.1016/S0076-6879(05)07056-4
DO - 10.1016/S0076-6879(05)07056-4
M3 - Article
C2 - 16757364
AN - SCOPUS:33744509562
VL - 407
SP - 711
EP - 721
JO - ImmunoMethods
JF - ImmunoMethods
SN - 1046-2023
ER -