TY - JOUR
T1 - Analyses of RAS Regulation of Eye Development in Drosophila melanogaster
AU - Firth, Lucy C.
AU - Li, Wei
AU - Zhang, Hui
AU - Baker, Nicholas E.
N1 - Funding Information:
We thank our colleagues in the Drosophila field for developing most of these reagents and methods, and David Tyler for comments. Research in our laboratory is supported by the National Institutes of Health and by the American Heart Association (Heritage Affiliate). NEB is a Scolar of the Irma T. Hirschl Fund for Biomedical Research.
Copyright:
Copyright 2019 Elsevier B.V., All rights reserved.
PY - 2006
Y1 - 2006
N2 - Many aspects of Drosophila eye development depend on receptor tyrosine kinases that signal through Ras. Genetic studies and genetic screens using eye morphology and development as assays have identified major components of receptor tyrosine kinase and Ras signaling and outlined specific contributions of these components to cell fate specification and differentiation, cell survival, cell cycle progression and arrest, and cellular movements and morphology. This chapter presents a brief compendium of methods and strains that may be used to obtain overexpression or loss of function for Ras pathway genes in the eye and methods and reagents permitting initial characterization of retinal cell differentiation, death, and cell cycle behavior.
AB - Many aspects of Drosophila eye development depend on receptor tyrosine kinases that signal through Ras. Genetic studies and genetic screens using eye morphology and development as assays have identified major components of receptor tyrosine kinase and Ras signaling and outlined specific contributions of these components to cell fate specification and differentiation, cell survival, cell cycle progression and arrest, and cellular movements and morphology. This chapter presents a brief compendium of methods and strains that may be used to obtain overexpression or loss of function for Ras pathway genes in the eye and methods and reagents permitting initial characterization of retinal cell differentiation, death, and cell cycle behavior.
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U2 - 10.1016/S0076-6879(05)07056-4
DO - 10.1016/S0076-6879(05)07056-4
M3 - Review article
C2 - 16757364
AN - SCOPUS:33744509562
VL - 407
SP - 711
EP - 721
JO - Methods in Enzymology
JF - Methods in Enzymology
SN - 0076-6879
ER -