An oxyferrous heme/protein-based radical intermediate is catalytically competent in the catalase reaction of Mycobacterium tuberculosis catalase-peroxidase (KatG)

Javier Suarez, Kalina Ranguelova, Andrzej A. Jarzecki, Julia Manzerova, Vladimir Krymov, Xiangbo Zhao, Shengwei Yu, Leonid Metlitsky, Gary J. Gerfen, Richard S. Magliozzo

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Abstract

A mechanism accounting for the robust catalase activity in catalase-peroxidases (KatG) presents a new challenge in heme protein enzymology. In Mycobacterium tuberculosis, KatG is the sole catalase and is also responsible for peroxidative activation of isoniazid, an anti-tuberculosis pro-drug. Here, optical stopped-flow spectrophotometry, rapid freeze-quench EPR spectroscopy both at the X-band and at the D-band, and mutagenesis are used to identify catalase reaction intermediates in M. tuberculosis KatG. In the presence of millimolar H2O2 at neutral pH, oxyferrous heme is formed within milliseconds from ferric (resting) KatG, whereas at pH 8.5, low spin ferric heme is formed. Using rapid freeze-quench EPR at X-band under both of these conditions, a narrow doublet radical signal with an 11 G principal hyperfine splitting was detected within the first milliseconds of turnover. The radical and the unique heme intermediates persist in wild-type KatG only during the time course of turnover of excess H2O2 (1000-fold or more). Mutation of Met255,Tyr229, or Trp107, which have covalently linked side chains in a unique distal side adduct (MYW) in wild-type KatG, abolishes this radical and the catalase activity. The D-band EPR spectrum of the radical exhibits a rhombic g tensor with dual gx values (2.00550 and 2.00606) and unique gy (2.00344) and g'z values (2.00186) similar to but not typical of native tyrosyl radicals. Density functional theory calculations based on a model of an MYW adduct radical built from x-ray coordinates predict experimentally observed hyperfine interactions and a shift in g values away from the native tyrosyl radical. A catalytic role for an MYW adduct radical in the catalase mechanism of KatG is proposed.

Original languageEnglish (US)
Pages (from-to)7017-7029
Number of pages13
JournalJournal of Biological Chemistry
Volume284
Issue number11
DOIs
StatePublished - Mar 13 2009

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Hemeproteins
Mycobacterium tuberculosis
Catalase
Peroxidase
Heme
Paramagnetic resonance
Peroxidases
Reaction intermediates
Mutagenesis
Optical flows
Spectrophotometry
Isoniazid
Prodrugs
Density functional theory
Tensors
Spectrum Analysis
Tuberculosis
Chemical activation
X-Rays
Spectroscopy

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

An oxyferrous heme/protein-based radical intermediate is catalytically competent in the catalase reaction of Mycobacterium tuberculosis catalase-peroxidase (KatG). / Suarez, Javier; Ranguelova, Kalina; Jarzecki, Andrzej A.; Manzerova, Julia; Krymov, Vladimir; Zhao, Xiangbo; Yu, Shengwei; Metlitsky, Leonid; Gerfen, Gary J.; Magliozzo, Richard S.

In: Journal of Biological Chemistry, Vol. 284, No. 11, 13.03.2009, p. 7017-7029.

Research output: Contribution to journalArticle

Suarez, Javier ; Ranguelova, Kalina ; Jarzecki, Andrzej A. ; Manzerova, Julia ; Krymov, Vladimir ; Zhao, Xiangbo ; Yu, Shengwei ; Metlitsky, Leonid ; Gerfen, Gary J. ; Magliozzo, Richard S. / An oxyferrous heme/protein-based radical intermediate is catalytically competent in the catalase reaction of Mycobacterium tuberculosis catalase-peroxidase (KatG). In: Journal of Biological Chemistry. 2009 ; Vol. 284, No. 11. pp. 7017-7029.
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AU - Jarzecki, Andrzej A.

AU - Manzerova, Julia

AU - Krymov, Vladimir

AU - Zhao, Xiangbo

AU - Yu, Shengwei

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