An overnight radioimmunoassay for human luteinizing hormone

Loretta P. Brown, Paige K. Besch, Veasy C. Buttram, Norman S. Fleischer, Dean S. Skelley

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A radioimmunoassay procedure for luteinizing hormone in serum or plasma has been developed in which the assay mixture is incubated at 37 °C. for a total of 22 hours rather than at 4 °C. for 4 to 7 days. The within assay coefficients of variation for the 37 °C. and 4 °C. methods, respectively, are 8.5 per cent and 4.7 per cent, while the between assay variations are 17.0 per cent and 15.1 per cent. Values at 4 °C. (y) were related to values at 37 °C. (x) according to the equation x = 1.4y + 0.6 with a correlation coefficient of 0.86.

Original languageEnglish (US)
Pages (from-to)129-133
Number of pages5
JournalThe Journal of Laboratory and Clinical Medicine
Volume84
Issue number1
StatePublished - 1974
Externally publishedYes

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Luteinizing Hormone
Radioimmunoassay
Assays
Serum
Plasmas

ASJC Scopus subject areas

  • Medicine(all)
  • Pathology and Forensic Medicine

Cite this

Brown, L. P., Besch, P. K., Buttram, V. C., Fleischer, N. S., & Skelley, D. S. (1974). An overnight radioimmunoassay for human luteinizing hormone. The Journal of Laboratory and Clinical Medicine, 84(1), 129-133.

An overnight radioimmunoassay for human luteinizing hormone. / Brown, Loretta P.; Besch, Paige K.; Buttram, Veasy C.; Fleischer, Norman S.; Skelley, Dean S.

In: The Journal of Laboratory and Clinical Medicine, Vol. 84, No. 1, 1974, p. 129-133.

Research output: Contribution to journalArticle

Brown, LP, Besch, PK, Buttram, VC, Fleischer, NS & Skelley, DS 1974, 'An overnight radioimmunoassay for human luteinizing hormone', The Journal of Laboratory and Clinical Medicine, vol. 84, no. 1, pp. 129-133.
Brown LP, Besch PK, Buttram VC, Fleischer NS, Skelley DS. An overnight radioimmunoassay for human luteinizing hormone. The Journal of Laboratory and Clinical Medicine. 1974;84(1):129-133.
Brown, Loretta P. ; Besch, Paige K. ; Buttram, Veasy C. ; Fleischer, Norman S. ; Skelley, Dean S. / An overnight radioimmunoassay for human luteinizing hormone. In: The Journal of Laboratory and Clinical Medicine. 1974 ; Vol. 84, No. 1. pp. 129-133.
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