An overnight radioimmunoassay for human luteinizing hormone

Loretta P. Brown; Paige K. Besch; Veasy C. Buttram; Norman S. Fleischer; Dean S. Skelley

An overnight radioimmunoassay for human luteinizing hormone

Loretta P. Brown, Paige K. Besch, Veasy C. Buttram, Norman S. Fleischer, Dean S. Skelley

Medicine

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

A radioimmunoassay procedure for luteinizing hormone in serum or plasma has been developed in which the assay mixture is incubated at 37 °C. for a total of 22 hours rather than at 4 °C. for 4 to 7 days. The within assay coefficients of variation for the 37 °C. and 4 °C. methods, respectively, are 8.5 per cent and 4.7 per cent, while the between assay variations are 17.0 per cent and 15.1 per cent. Values at 4 °C. (y) were related to values at 37 °C. (x) according to the equation x = 1.4y + 0.6 with a correlation coefficient of 0.86.

Original language	English (US)
Pages (from-to)	129-133
Number of pages	5
Journal	The Journal of Laboratory and Clinical Medicine
Volume	84
Issue number	1
State	Published - Jul 1974

ASJC Scopus subject areas

Pathology and Forensic Medicine

Cite this

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title = "An overnight radioimmunoassay for human luteinizing hormone",

abstract = "A radioimmunoassay procedure for luteinizing hormone in serum or plasma has been developed in which the assay mixture is incubated at 37 °C. for a total of 22 hours rather than at 4 °C. for 4 to 7 days. The within assay coefficients of variation for the 37 °C. and 4 °C. methods, respectively, are 8.5 per cent and 4.7 per cent, while the between assay variations are 17.0 per cent and 15.1 per cent. Values at 4 °C. (y) were related to values at 37 °C. (x) according to the equation x = 1.4y + 0.6 with a correlation coefficient of 0.86.",

author = "Brown, {Loretta P.} and Besch, {Paige K.} and Buttram, {Veasy C.} and Fleischer, {Norman S.} and Skelley, {Dean S.}",

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AU - Brown, Loretta P.

AU - Besch, Paige K.

AU - Buttram, Veasy C.

AU - Fleischer, Norman S.

AU - Skelley, Dean S.

PY - 1974/7

Y1 - 1974/7

N2 - A radioimmunoassay procedure for luteinizing hormone in serum or plasma has been developed in which the assay mixture is incubated at 37 °C. for a total of 22 hours rather than at 4 °C. for 4 to 7 days. The within assay coefficients of variation for the 37 °C. and 4 °C. methods, respectively, are 8.5 per cent and 4.7 per cent, while the between assay variations are 17.0 per cent and 15.1 per cent. Values at 4 °C. (y) were related to values at 37 °C. (x) according to the equation x = 1.4y + 0.6 with a correlation coefficient of 0.86.

AB - A radioimmunoassay procedure for luteinizing hormone in serum or plasma has been developed in which the assay mixture is incubated at 37 °C. for a total of 22 hours rather than at 4 °C. for 4 to 7 days. The within assay coefficients of variation for the 37 °C. and 4 °C. methods, respectively, are 8.5 per cent and 4.7 per cent, while the between assay variations are 17.0 per cent and 15.1 per cent. Values at 4 °C. (y) were related to values at 37 °C. (x) according to the equation x = 1.4y + 0.6 with a correlation coefficient of 0.86.

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An overnight radioimmunoassay for human luteinizing hormone

Abstract

ASJC Scopus subject areas

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