An overnight radioimmunoassay for human luteinizing hormone

Loretta P. Brown, Paige K. Besch, Veasy C. Buttram, Norman S. Fleischer, Dean S. Skelley

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Abstract

A radioimmunoassay procedure for luteinizing hormone in serum or plasma has been developed in which the assay mixture is incubated at 37 °C. for a total of 22 hours rather than at 4 °C. for 4 to 7 days. The within assay coefficients of variation for the 37 °C. and 4 °C. methods, respectively, are 8.5 per cent and 4.7 per cent, while the between assay variations are 17.0 per cent and 15.1 per cent. Values at 4 °C. (y) were related to values at 37 °C. (x) according to the equation x = 1.4y + 0.6 with a correlation coefficient of 0.86.

Original languageEnglish (US)
Pages (from-to)129-133
Number of pages5
JournalThe Journal of Laboratory and Clinical Medicine
Volume84
Issue number1
StatePublished - Jul 1974

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ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Brown, L. P., Besch, P. K., Buttram, V. C., Fleischer, N. S., & Skelley, D. S. (1974). An overnight radioimmunoassay for human luteinizing hormone. The Journal of Laboratory and Clinical Medicine, 84(1), 129-133.