An integrated map of the genome of the tubercle bacillus, Mycobacterium tuberculosis H37Rv, and comparison with Mycobacterium leprae

Wolfgang J. Philipp, Sylvie Poulet, Karin Eiglmeier, Lisa Pascopella, V. Balasubramanian, Beate Heym, Staffan Bergh, Barry R. Bloom, William R. Jacobs, Stewart T. Cole

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Abstract

An integrated map of the genome of the tubercle bacillus. Mycobacterium tuberculosis, was constructed by using a twin-pronged approach. Pulsed-field gel electrophoretic analysis enabled cleavage sites for Asn I and Dra I to be positioned on the 4.4-Mb circular chromosome, while, in parallel, clones from two cosmid libraries were ordered into contigs by means of fingerprinting and hybridization mapping. The resultant contig map was readily correlated with the physical map of the genome via the landmarked restriction sites. Over 165 genes and markers were localized on the integrated map, thus enabling comparisons with the leprosy bacillus, Mycobacterium leprae, to be undertaken. Mycobacterial genomes appear to have evolved as mosaic structures since extended segments with conserved gene order and organization are interspersed with different flanking regions. Repetitive sequences and insertion elements are highly abundant in M. tuberculosis, but the distribution of IS6110 is apparently nonrandom.

Original languageEnglish (US)
Pages (from-to)3132-3137
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume93
Issue number7
DOIs
Publication statusPublished - Apr 2 1996

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Keywords

  • bacterial genomics
  • contig mapping
  • genome mapping
  • ordered libraries
  • tuberculosis

ASJC Scopus subject areas

  • General

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