An integrated map of the genome of the tubercle bacillus, Mycobacterium tuberculosis H37Rv, and comparison with Mycobacterium leprae

Wolfgang J. Philipp, Sylvie Poulet, Karin Eiglmeier, Lisa Pascopella, V. Balasubramanian, Beate Heym, Staffan Bergh, Barry R. Bloom, William R. Jacobs, Stewart T. Cole

Research output: Contribution to journalArticle

228 Citations (Scopus)

Abstract

An integrated map of the genome of the tubercle bacillus. Mycobacterium tuberculosis, was constructed by using a twin-pronged approach. Pulsed-field gel electrophoretic analysis enabled cleavage sites for Asn I and Dra I to be positioned on the 4.4-Mb circular chromosome, while, in parallel, clones from two cosmid libraries were ordered into contigs by means of fingerprinting and hybridization mapping. The resultant contig map was readily correlated with the physical map of the genome via the landmarked restriction sites. Over 165 genes and markers were localized on the integrated map, thus enabling comparisons with the leprosy bacillus, Mycobacterium leprae, to be undertaken. Mycobacterial genomes appear to have evolved as mosaic structures since extended segments with conserved gene order and organization are interspersed with different flanking regions. Repetitive sequences and insertion elements are highly abundant in M. tuberculosis, but the distribution of IS6110 is apparently nonrandom.

Original languageEnglish (US)
Pages (from-to)3132-3137
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume93
Issue number7
DOIs
StatePublished - Apr 2 1996

Fingerprint

Mycobacterium leprae
Mycobacterium tuberculosis
Bacillus
Genome
Cosmids
Gene Order
Nucleic Acid Repetitive Sequences
Insertional Mutagenesis
Leprosy
Clone Cells
Chromosomes
Gels
Genes

Keywords

  • bacterial genomics
  • contig mapping
  • genome mapping
  • ordered libraries
  • tuberculosis

ASJC Scopus subject areas

  • General

Cite this

An integrated map of the genome of the tubercle bacillus, Mycobacterium tuberculosis H37Rv, and comparison with Mycobacterium leprae. / Philipp, Wolfgang J.; Poulet, Sylvie; Eiglmeier, Karin; Pascopella, Lisa; Balasubramanian, V.; Heym, Beate; Bergh, Staffan; Bloom, Barry R.; Jacobs, William R.; Cole, Stewart T.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, No. 7, 02.04.1996, p. 3132-3137.

Research output: Contribution to journalArticle

Philipp, Wolfgang J. ; Poulet, Sylvie ; Eiglmeier, Karin ; Pascopella, Lisa ; Balasubramanian, V. ; Heym, Beate ; Bergh, Staffan ; Bloom, Barry R. ; Jacobs, William R. ; Cole, Stewart T. / An integrated map of the genome of the tubercle bacillus, Mycobacterium tuberculosis H37Rv, and comparison with Mycobacterium leprae. In: Proceedings of the National Academy of Sciences of the United States of America. 1996 ; Vol. 93, No. 7. pp. 3132-3137.
@article{069c819de92e486c960c1fe43d30be75,
title = "An integrated map of the genome of the tubercle bacillus, Mycobacterium tuberculosis H37Rv, and comparison with Mycobacterium leprae",
abstract = "An integrated map of the genome of the tubercle bacillus. Mycobacterium tuberculosis, was constructed by using a twin-pronged approach. Pulsed-field gel electrophoretic analysis enabled cleavage sites for Asn I and Dra I to be positioned on the 4.4-Mb circular chromosome, while, in parallel, clones from two cosmid libraries were ordered into contigs by means of fingerprinting and hybridization mapping. The resultant contig map was readily correlated with the physical map of the genome via the landmarked restriction sites. Over 165 genes and markers were localized on the integrated map, thus enabling comparisons with the leprosy bacillus, Mycobacterium leprae, to be undertaken. Mycobacterial genomes appear to have evolved as mosaic structures since extended segments with conserved gene order and organization are interspersed with different flanking regions. Repetitive sequences and insertion elements are highly abundant in M. tuberculosis, but the distribution of IS6110 is apparently nonrandom.",
keywords = "bacterial genomics, contig mapping, genome mapping, ordered libraries, tuberculosis",
author = "Philipp, {Wolfgang J.} and Sylvie Poulet and Karin Eiglmeier and Lisa Pascopella and V. Balasubramanian and Beate Heym and Staffan Bergh and Bloom, {Barry R.} and Jacobs, {William R.} and Cole, {Stewart T.}",
year = "1996",
month = "4",
day = "2",
doi = "10.1073/pnas.93.7.3132",
language = "English (US)",
volume = "93",
pages = "3132--3137",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "7",

}

TY - JOUR

T1 - An integrated map of the genome of the tubercle bacillus, Mycobacterium tuberculosis H37Rv, and comparison with Mycobacterium leprae

AU - Philipp, Wolfgang J.

AU - Poulet, Sylvie

AU - Eiglmeier, Karin

AU - Pascopella, Lisa

AU - Balasubramanian, V.

AU - Heym, Beate

AU - Bergh, Staffan

AU - Bloom, Barry R.

AU - Jacobs, William R.

AU - Cole, Stewart T.

PY - 1996/4/2

Y1 - 1996/4/2

N2 - An integrated map of the genome of the tubercle bacillus. Mycobacterium tuberculosis, was constructed by using a twin-pronged approach. Pulsed-field gel electrophoretic analysis enabled cleavage sites for Asn I and Dra I to be positioned on the 4.4-Mb circular chromosome, while, in parallel, clones from two cosmid libraries were ordered into contigs by means of fingerprinting and hybridization mapping. The resultant contig map was readily correlated with the physical map of the genome via the landmarked restriction sites. Over 165 genes and markers were localized on the integrated map, thus enabling comparisons with the leprosy bacillus, Mycobacterium leprae, to be undertaken. Mycobacterial genomes appear to have evolved as mosaic structures since extended segments with conserved gene order and organization are interspersed with different flanking regions. Repetitive sequences and insertion elements are highly abundant in M. tuberculosis, but the distribution of IS6110 is apparently nonrandom.

AB - An integrated map of the genome of the tubercle bacillus. Mycobacterium tuberculosis, was constructed by using a twin-pronged approach. Pulsed-field gel electrophoretic analysis enabled cleavage sites for Asn I and Dra I to be positioned on the 4.4-Mb circular chromosome, while, in parallel, clones from two cosmid libraries were ordered into contigs by means of fingerprinting and hybridization mapping. The resultant contig map was readily correlated with the physical map of the genome via the landmarked restriction sites. Over 165 genes and markers were localized on the integrated map, thus enabling comparisons with the leprosy bacillus, Mycobacterium leprae, to be undertaken. Mycobacterial genomes appear to have evolved as mosaic structures since extended segments with conserved gene order and organization are interspersed with different flanking regions. Repetitive sequences and insertion elements are highly abundant in M. tuberculosis, but the distribution of IS6110 is apparently nonrandom.

KW - bacterial genomics

KW - contig mapping

KW - genome mapping

KW - ordered libraries

KW - tuberculosis

UR - http://www.scopus.com/inward/record.url?scp=2642685965&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2642685965&partnerID=8YFLogxK

U2 - 10.1073/pnas.93.7.3132

DO - 10.1073/pnas.93.7.3132

M3 - Article

C2 - 8610181

AN - SCOPUS:2642685965

VL - 93

SP - 3132

EP - 3137

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 7

ER -