An immunological study on chromogranin A and B in human endocrine and nervous tissues

K. W. Schmid, R. Weiler, R. W. Xu, R. Hogue-Angeletti, R. Fischer-Colbrie, H. Winkler

Research output: Contribution to journalArticle

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Abstract

Antisera were raised against synthetic peptides derived from the primary amino acid sequence of human chromogranin B. These antisera recognized in one- and two-dimensional immunoblotting a component previously designated as chromogranin B. In human chromaffin granules, the major endogenous processing product of chromogranin B is formed by proteolytic cleavage of the protein near the C-terminus. Immunohistochemical localizations were obtained with antisera against human chromogranins A and B and against a synthetic peptide corresponding to the B sequence. In human tissues, chromogranin B is co-stored with chromogranin A in the adrenal medulla, the anterior pituitary, parafollicular cells of the thyroid, in some cells of the endocrine pancreas and in some enterochromaffin cells, whereas only chromogranin A is found in the parathyroid gland and enterochromaffin cells of the gastric corpus mucosa. In the nervous system, no immunostaining was observed for chromogranin A and only a weak one for chromogranin B in some cells of the spinal cord. However, the Purkinje cells of the cerebellum were strongly positive for chromogranin B.

Original languageEnglish (US)
Pages (from-to)365-372
Number of pages8
JournalThe Histochemical Journal
Volume21
Issue number6
DOIs
StatePublished - Jun 1989
Externally publishedYes

Fingerprint

Chromogranin B
Nerve Tissue
Chromogranin A
Enterochromaffin Cells
Immune Sera
Chromaffin Granules
Peptides
Adrenal Medulla
Parathyroid Glands
Purkinje Cells
Gastric Mucosa
Protein C
Islets of Langerhans
Immunoblotting
Cerebellum
Nervous System
Amino Acid Sequence
Spinal Cord
Thyroid Gland
human CHGB protein

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Schmid, K. W., Weiler, R., Xu, R. W., Hogue-Angeletti, R., Fischer-Colbrie, R., & Winkler, H. (1989). An immunological study on chromogranin A and B in human endocrine and nervous tissues. The Histochemical Journal, 21(6), 365-372. https://doi.org/10.1007/BF01798500

An immunological study on chromogranin A and B in human endocrine and nervous tissues. / Schmid, K. W.; Weiler, R.; Xu, R. W.; Hogue-Angeletti, R.; Fischer-Colbrie, R.; Winkler, H.

In: The Histochemical Journal, Vol. 21, No. 6, 06.1989, p. 365-372.

Research output: Contribution to journalArticle

Schmid, KW, Weiler, R, Xu, RW, Hogue-Angeletti, R, Fischer-Colbrie, R & Winkler, H 1989, 'An immunological study on chromogranin A and B in human endocrine and nervous tissues', The Histochemical Journal, vol. 21, no. 6, pp. 365-372. https://doi.org/10.1007/BF01798500
Schmid KW, Weiler R, Xu RW, Hogue-Angeletti R, Fischer-Colbrie R, Winkler H. An immunological study on chromogranin A and B in human endocrine and nervous tissues. The Histochemical Journal. 1989 Jun;21(6):365-372. https://doi.org/10.1007/BF01798500
Schmid, K. W. ; Weiler, R. ; Xu, R. W. ; Hogue-Angeletti, R. ; Fischer-Colbrie, R. ; Winkler, H. / An immunological study on chromogranin A and B in human endocrine and nervous tissues. In: The Histochemical Journal. 1989 ; Vol. 21, No. 6. pp. 365-372.
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