An atypical type II topoisomerase from Mycobacterium smegmatis with positive supercoiling activity

Paras Jain, Valakunja Nagaraja

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Topoisomerases are essential ubiquitous enzymes, falling into two distinct classes. A number of eubacteria including Escherichia coli, typically contain four topoisomerases, two type I topoisomerases and two type II topoisomerases viz. DNA gyrase and topoisomerase IV. In contrast several other bacterial genomes including mycobacteria, encode for one type I topoisomerase and a DNA gyrase. Here we describe a new type II topoisomerase from Mycobacterium smegmatis which is different from DNA gyrase or topoisomerase IV in its characteristics and origin. The topoisomerase is distinct with respect to domain organization, properties and drug sensitivity. The enzyme catalyses relaxation of negatively supercoiled DNA in an ATP-dependent manner and also introduces positive supercoils to both relaxed and negatively supercoiled substrates. The genes for this additional topoisomerase are not found in other sequenced mycobacterial genomes and may represent a distant lineage.

Original languageEnglish (US)
Pages (from-to)1392-1405
Number of pages14
JournalMolecular Microbiology
Volume58
Issue number5
DOIs
StatePublished - Dec 2005
Externally publishedYes

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Mycobacterium smegmatis
Type II DNA Topoisomerase
Type I DNA Topoisomerase
DNA Gyrase
DNA Topoisomerase IV
Superhelical DNA
Bacterial Genomes
Enzymes
Mycobacterium
Adenosine Triphosphate
Genome
Escherichia coli
Bacteria
Pharmaceutical Preparations
Genes

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

Cite this

An atypical type II topoisomerase from Mycobacterium smegmatis with positive supercoiling activity. / Jain, Paras; Nagaraja, Valakunja.

In: Molecular Microbiology, Vol. 58, No. 5, 12.2005, p. 1392-1405.

Research output: Contribution to journalArticle

Jain, Paras ; Nagaraja, Valakunja. / An atypical type II topoisomerase from Mycobacterium smegmatis with positive supercoiling activity. In: Molecular Microbiology. 2005 ; Vol. 58, No. 5. pp. 1392-1405.
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