Abstract
Using a new procedure that combines electron-density correlation with biochemical information, we have fitted the crystal structure of the N- terminal actin-binding domain of human T-fimbrin to helical reconstructions of fimbrin-decorated actin filaments. The map locates the N-terminal calcium- binding domain and identifies actin-binding site residues on the two calponin-homology domains of fimbrin. Based on this map, we propose a model of a fimbrin crosslink in an actin bundle and its regulation by calcium.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 787-792 |
| Number of pages | 6 |
| Journal | Nature Structural Biology |
| Volume | 5 |
| Issue number | 9 |
| DOIs | |
| State | Published - 1998 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Structural Biology
- Genetics
Cite this
An atomic model of fimbrin binding to F-actin and its implications for filament crosslinking and regulation. / Hanein, D.; Volkmann, N.; Goldsmith, S.; Michon, A. M.; Lehman, W.; Craig, R.; DeRosier, D.; Almo, Steven C.; Matsudaira, P.
In: Nature Structural Biology, Vol. 5, No. 9, 1998, p. 787-792.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - An atomic model of fimbrin binding to F-actin and its implications for filament crosslinking and regulation
AU - Hanein, D.
AU - Volkmann, N.
AU - Goldsmith, S.
AU - Michon, A. M.
AU - Lehman, W.
AU - Craig, R.
AU - DeRosier, D.
AU - Almo, Steven C.
AU - Matsudaira, P.
PY - 1998
Y1 - 1998
N2 - Using a new procedure that combines electron-density correlation with biochemical information, we have fitted the crystal structure of the N- terminal actin-binding domain of human T-fimbrin to helical reconstructions of fimbrin-decorated actin filaments. The map locates the N-terminal calcium- binding domain and identifies actin-binding site residues on the two calponin-homology domains of fimbrin. Based on this map, we propose a model of a fimbrin crosslink in an actin bundle and its regulation by calcium.
AB - Using a new procedure that combines electron-density correlation with biochemical information, we have fitted the crystal structure of the N- terminal actin-binding domain of human T-fimbrin to helical reconstructions of fimbrin-decorated actin filaments. The map locates the N-terminal calcium- binding domain and identifies actin-binding site residues on the two calponin-homology domains of fimbrin. Based on this map, we propose a model of a fimbrin crosslink in an actin bundle and its regulation by calcium.
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UR - http://www.scopus.com/inward/citedby.url?scp=0031694478&partnerID=8YFLogxK
U2 - 10.1038/1828
DO - 10.1038/1828
M3 - Article
VL - 5
SP - 787
EP - 792
JO - Nature Structural and Molecular Biology
JF - Nature Structural and Molecular Biology
SN - 1545-9993
IS - 9
ER -