An atomic model of fimbrin binding to F-actin and its implications for filament crosslinking and regulation

D. Hanein, N. Volkmann, S. Goldsmith, A. M. Michon, W. Lehman, R. Craig, D. DeRosier, Steven C. Almo, P. Matsudaira

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

Using a new procedure that combines electron-density correlation with biochemical information, we have fitted the crystal structure of the N- terminal actin-binding domain of human T-fimbrin to helical reconstructions of fimbrin-decorated actin filaments. The map locates the N-terminal calcium- binding domain and identifies actin-binding site residues on the two calponin-homology domains of fimbrin. Based on this map, we propose a model of a fimbrin crosslink in an actin bundle and its regulation by calcium.

Original languageEnglish (US)
Pages (from-to)787-792
Number of pages6
JournalNature Structural Biology
Volume5
Issue number9
DOIs
StatePublished - 1998
Externally publishedYes

Fingerprint

Crosslinking
Actins
Calcium
Actin Cytoskeleton
Carrier concentration
Crystal structure
Binding Sites
Electrons
plastin

ASJC Scopus subject areas

  • Biochemistry
  • Structural Biology
  • Genetics

Cite this

Hanein, D., Volkmann, N., Goldsmith, S., Michon, A. M., Lehman, W., Craig, R., ... Matsudaira, P. (1998). An atomic model of fimbrin binding to F-actin and its implications for filament crosslinking and regulation. Nature Structural Biology, 5(9), 787-792. https://doi.org/10.1038/1828

An atomic model of fimbrin binding to F-actin and its implications for filament crosslinking and regulation. / Hanein, D.; Volkmann, N.; Goldsmith, S.; Michon, A. M.; Lehman, W.; Craig, R.; DeRosier, D.; Almo, Steven C.; Matsudaira, P.

In: Nature Structural Biology, Vol. 5, No. 9, 1998, p. 787-792.

Research output: Contribution to journalArticle

Hanein, D, Volkmann, N, Goldsmith, S, Michon, AM, Lehman, W, Craig, R, DeRosier, D, Almo, SC & Matsudaira, P 1998, 'An atomic model of fimbrin binding to F-actin and its implications for filament crosslinking and regulation', Nature Structural Biology, vol. 5, no. 9, pp. 787-792. https://doi.org/10.1038/1828
Hanein, D. ; Volkmann, N. ; Goldsmith, S. ; Michon, A. M. ; Lehman, W. ; Craig, R. ; DeRosier, D. ; Almo, Steven C. ; Matsudaira, P. / An atomic model of fimbrin binding to F-actin and its implications for filament crosslinking and regulation. In: Nature Structural Biology. 1998 ; Vol. 5, No. 9. pp. 787-792.
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