An atomic model of fimbrin binding to F-actin and its implications for filament crosslinking and regulation

D. Hanein; N. Volkmann; S. Goldsmith; A. M. Michon; W. Lehman; R. Craig; D. DeRosier; S. Almo; P. Matsudaira

doi:10.1038/1828

An atomic model of fimbrin binding to F-actin and its implications for filament crosslinking and regulation

D. Hanein, N. Volkmann, S. Goldsmith, A. M. Michon, W. Lehman, R. Craig, D. DeRosier, S. Almo, P. Matsudaira

Research output: Contribution to journal › Article

105 Scopus citations

Abstract

Using a new procedure that combines electron-density correlation with biochemical information, we have fitted the crystal structure of the N- terminal actin-binding domain of human T-fimbrin to helical reconstructions of fimbrin-decorated actin filaments. The map locates the N-terminal calcium- binding domain and identifies actin-binding site residues on the two calponin-homology domains of fimbrin. Based on this map, we propose a model of a fimbrin crosslink in an actin bundle and its regulation by calcium.

Original language	English (US)
Pages (from-to)	787-792
Number of pages	6
Journal	Nature structural biology
Volume	5
Issue number	9
DOIs	https://doi.org/10.1038/1828
Publication status	Published - Sep 9 1998
Externally published	Yes

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ASJC Scopus subject areas

Structural Biology
Biochemistry
Genetics

Cite this

Hanein, D., Volkmann, N., Goldsmith, S., Michon, A. M., Lehman, W., Craig, R., ... Matsudaira, P. (1998). An atomic model of fimbrin binding to F-actin and its implications for filament crosslinking and regulation. Nature structural biology, 5(9), 787-792. https://doi.org/10.1038/1828

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AU - Lehman, W.

AU - Craig, R.

AU - DeRosier, D.

AU - Almo, S.

AU - Matsudaira, P.

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10.1038/1828