An atomic model of fimbrin binding to F-actin and its implications for filament crosslinking and regulation

D. Hanein, N. Volkmann, S. Goldsmith, A. M. Michon, W. Lehman, R. Craig, D. DeRosier, S. Almo, P. Matsudaira

Research output: Contribution to journalArticle

106 Scopus citations

Abstract

Using a new procedure that combines electron-density correlation with biochemical information, we have fitted the crystal structure of the N- terminal actin-binding domain of human T-fimbrin to helical reconstructions of fimbrin-decorated actin filaments. The map locates the N-terminal calcium- binding domain and identifies actin-binding site residues on the two calponin-homology domains of fimbrin. Based on this map, we propose a model of a fimbrin crosslink in an actin bundle and its regulation by calcium.

Original languageEnglish (US)
Pages (from-to)787-792
Number of pages6
JournalNature structural biology
Volume5
Issue number9
DOIs
StatePublished - Sep 9 1998
Externally publishedYes

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Genetics

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    Hanein, D., Volkmann, N., Goldsmith, S., Michon, A. M., Lehman, W., Craig, R., DeRosier, D., Almo, S., & Matsudaira, P. (1998). An atomic model of fimbrin binding to F-actin and its implications for filament crosslinking and regulation. Nature structural biology, 5(9), 787-792. https://doi.org/10.1038/1828