An atomic model of fimbrin binding to F-actin and its implications for filament crosslinking and regulation

D. Hanein; N. Volkmann; S. Goldsmith; A. M. Michon; W. Lehman; R. Craig; D. DeRosier; S. Almo; P. Matsudaira

doi:10.1038/1828

An atomic model of fimbrin binding to F-actin and its implications for filament crosslinking and regulation

D. Hanein, N. Volkmann, S. Goldsmith, A. M. Michon, W. Lehman, R. Craig, D. DeRosier, S. Almo, P. Matsudaira

Research output: Contribution to journal › Article › peer-review

106 Scopus citations

Abstract

Using a new procedure that combines electron-density correlation with biochemical information, we have fitted the crystal structure of the N- terminal actin-binding domain of human T-fimbrin to helical reconstructions of fimbrin-decorated actin filaments. The map locates the N-terminal calcium- binding domain and identifies actin-binding site residues on the two calponin-homology domains of fimbrin. Based on this map, we propose a model of a fimbrin crosslink in an actin bundle and its regulation by calcium.

Original language	English (US)
Pages (from-to)	787-792
Number of pages	6
Journal	Nature structural biology
Volume	5
Issue number	9
DOIs	https://doi.org/10.1038/1828
State	Published - Sep 9 1998
Externally published	Yes

ASJC Scopus subject areas

Structural Biology
Biochemistry
Genetics

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title = "An atomic model of fimbrin binding to F-actin and its implications for filament crosslinking and regulation",

abstract = "Using a new procedure that combines electron-density correlation with biochemical information, we have fitted the crystal structure of the N- terminal actin-binding domain of human T-fimbrin to helical reconstructions of fimbrin-decorated actin filaments. The map locates the N-terminal calcium- binding domain and identifies actin-binding site residues on the two calponin-homology domains of fimbrin. Based on this map, we propose a model of a fimbrin crosslink in an actin bundle and its regulation by calcium.",

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AU - Hanein, D.

AU - Volkmann, N.

AU - Goldsmith, S.

AU - Michon, A. M.

AU - Lehman, W.

AU - Craig, R.

AU - DeRosier, D.

AU - Almo, S.

AU - Matsudaira, P.

PY - 1998/9/9

Y1 - 1998/9/9

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AB - Using a new procedure that combines electron-density correlation with biochemical information, we have fitted the crystal structure of the N- terminal actin-binding domain of human T-fimbrin to helical reconstructions of fimbrin-decorated actin filaments. The map locates the N-terminal calcium- binding domain and identifies actin-binding site residues on the two calponin-homology domains of fimbrin. Based on this map, we propose a model of a fimbrin crosslink in an actin bundle and its regulation by calcium.

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