An antibody (AS/7) prepared against the carboxyl-terminal decapeptide of the α subunit of transducin (α(T)) has been used in various reconstitution studies aimed at characterizing the role of the carboxyl-terminal domain in the different functional activities of transducin. The peptide-specific antibody is a potent inhibitor of the rhodopsin-stimulated GTPase activity in phospholipid vesicle systems containing pure rhodopsin and pure holo-transducin, or rhodopsin and the purified α(T) and β/γ (βγ(T)) subunit components, with the highest levels of inhibition (80-95%) occurring under conditions where the molar ratio of holo-transducin (or α(T)) to AS/7 ≃ 1. The inhibition of the receptor-stimulated GTPase does not represent an interference in the interactions between the α(T) subunit and the βγ(T) complex, since essentially identical levels of inhibition are observed when AS/7 is preincubated with either free α(T), holo-transducin, or α(T) in the presence of excess βγ(T), prior to assay. The AS/7-induced inhibition also does not appear to reflect an alteration in the ability of α(T) to bind or hydrolyze GTP and, in fact, the incubation of α(T) with AS/7 results in a stimulation of the intrinsic GTPase activity for α(T) alone (i.e. in the absence of rhodopsin). Thus, we conclude that the inhibition of the rhodopsin-stimulated GTPase activity by AS/7 is due to the direct blocking (by the antibody) of rhodopsin-α(T) interactions. While AS/7 is capable of uncoupling rhodopsin-transducin interactions, it appears to promote the stimulation of the cyclic GMP phosphodiesterase (PDE) by an activated α(T) subunit. Specifically, when the pure α(T)-guanosine 5-O-(3-thiotriphosphate) (α(T)GTPγS) species is preincubated with AS/7 prior to its addition to an assay solution containing PDE, there is at least a 4-fold increase in the resultant cyclic GMP hydrolysis relative to the activities measured with α(T)GTPγS, alone, or with α(T)GTPγS preincubated with nonimmune (control) rabbit IgG. The AS/7-induced promotion is specific for the active form of α(T); the inactive α(T)GDP species does not stimulate PDE activity either in the presence or absence of the antibody. The different effects by AS/7 on the various activities of the α(T) subunit highlight the existence of distinct functional domains on α(T). In addition these results suggest that the interactions (by the photoreceptor) at the carboxyl-terminal domain of α(T) are not only essential for rhodopsin-transducin coupling but may also serve to facilitate the effective coupling of an activated α(T) subunit to the effector enzyme, PDE.
|Original language||English (US)|
|Number of pages||8|
|Journal||Journal of Biological Chemistry|
|State||Published - 1988|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology