An analysis of the microsporidian genus Brachiola, with comparisons of human and insect isolates of Brachiola algerae

Ann Cali, Louis M. Weiss, Peter M. Takvorian

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The genus Brachiola is the newest microsporidian genus established for a human infection with the type species being B. vesicularum in skeletal muscle. Subsequently, the microsporidium, Nosema algerae, identified from mosquitoes, was added to this genus because of morphological and physiological similarities. The present report illustrates a confirmed case of Brachiola algerae infecting skeletal muscle in a 56-year-old woman who was being treated for rheumatoid arthritis with immunosuppressive drugs. In the following study, these two human-infecting microsporidian species are ultrastructurally compared from human biopsy tissue. Additionally, Brachiola algerae from mosquitoes as reference B. algerae, was grown in athymic mice and compared to the human isolate in vivo, and in culture. B. algerae is morphologically identical in the host situations presented and different from B. vesicularum in human skeletal muscle. B. algerae has a consistently, slightly longer spore that typically contains one row of polar filament coils, while B. vesicularum typically contains two rows of polar filament coils and occasionally, one or three rows. In proliferative development, B. vesicularum forms protoplasmic extensions which do not occur on B. algerae, nor have they been reported on any other microsporidium. This report demonstrates that B. vesicularum and B. algerae are two different species of Brachiola that infect human skeletal muscle.

Original languageEnglish (US)
Pages (from-to)678-685
Number of pages8
JournalJournal of Eukaryotic Microbiology
Volume51
Issue number6
DOIs
StatePublished - Nov 2004

Fingerprint

Microsporidia
Insects
insects
skeletal muscle
Unclassified Microsporidia
Skeletal Muscle
Culicidae
Nosema algerae
Nosema
immunosuppressive agents
rheumatoid arthritis
Immunosuppressive Agents
Spores
Nude Mice
biopsy
Rheumatoid Arthritis
spores
Biopsy
drugs
mice

Keywords

  • Human myositis
  • Microspora
  • Microsporidia
  • Opportunistic infections

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Applied Microbiology and Biotechnology
  • Agricultural and Biological Sciences (miscellaneous)
  • Microbiology

Cite this

An analysis of the microsporidian genus Brachiola, with comparisons of human and insect isolates of Brachiola algerae. / Cali, Ann; Weiss, Louis M.; Takvorian, Peter M.

In: Journal of Eukaryotic Microbiology, Vol. 51, No. 6, 11.2004, p. 678-685.

Research output: Contribution to journalArticle

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