AML1-ETO decreases ETO-2 (MTG16) interactions with nuclear receptor corepressor, an effect that impairs granulocyte differentiation

Vinzon Ibañez, Arun Sharma, Silvia Buonamici, Amit K. Verma, Sudhakar Kalakonda, Jianxiang Wang, ShriHari Kadkol, Yogen Saunthararajah

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The t(8;21) chromosome abnormality in acute myeloid leukemia targets the AML1 and ETO genes to produce the leukemia fusion protein AML1-ETO. Another member of the ETO family, ETO-2/MTG16, is highly expressed in murine and human hematopoietic cells, bears >75% homology to ETO, and like ETO, contains a conserved MYND domain that interacts with the nuclear receptor corepressor (N-CoR). AML1-ETO prevents granulocyte but not macrophage differentiation of murine 32Dcl3 granulocyte/macrophage progenitors. One possible mechanism is recruitment of N-CoR to aberrantly repress AML1 target genes. We wished to examine another mechanism by which AML1-ETO might impair granulocyte differentiation. We demonstrate that AML1-ETO decreases interactions between ETO-2 and N-CoR. Furthermore, overexpression of ETO-2 relieves AML1-ETO-induced granulocyte differentiation arrest. This suggests that decreased interactions between ETO-2 and N-CoR may contribute to granulocyte differentiation impairment. The MYND domain coimmunoprecipitates with N-CoR and inhibits interactions between ETO-2 and N-CoR, presumably by occupying the ETO-2 binding site on N-CoR. This inhibition of ETO-2 interactions with N-CoR is specific because the MYND domain does not inhibit retinoic acid receptor interactions with N-CoR. To examine the effect of decreasing interactions between ETO-2 and N-CoR in hematopoietic cells, without effects of AML1-ETO such as direct repression of AML1 target genes, the MYND domain was expressed in 32Dcl3 and human CD34+ cells. The MYND domain prevented granulocyte but not macrophage differentiation of both 32Dcl3 and human CD34+ cells, recapitulating this effect of AML1-ETO. In conclusion, decreasing interactions between ETO-2 and N-CoR, an effect of AML1-ETO, inhibits granulocyte differentiation.

Original languageEnglish (US)
Pages (from-to)4547-4554
Number of pages8
JournalCancer Research
Volume64
Issue number13
DOIs
StatePublished - Jul 1 2004
Externally publishedYes

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Co-Repressor Proteins
Granulocytes
Macrophages
Genes
Granulocyte-Macrophage Progenitor Cells
Retinoic Acid Receptors
Acute Myeloid Leukemia
Chromosome Aberrations
Leukemia
Binding Sites

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

AML1-ETO decreases ETO-2 (MTG16) interactions with nuclear receptor corepressor, an effect that impairs granulocyte differentiation. / Ibañez, Vinzon; Sharma, Arun; Buonamici, Silvia; Verma, Amit K.; Kalakonda, Sudhakar; Wang, Jianxiang; Kadkol, ShriHari; Saunthararajah, Yogen.

In: Cancer Research, Vol. 64, No. 13, 01.07.2004, p. 4547-4554.

Research output: Contribution to journalArticle

Ibañez, Vinzon ; Sharma, Arun ; Buonamici, Silvia ; Verma, Amit K. ; Kalakonda, Sudhakar ; Wang, Jianxiang ; Kadkol, ShriHari ; Saunthararajah, Yogen. / AML1-ETO decreases ETO-2 (MTG16) interactions with nuclear receptor corepressor, an effect that impairs granulocyte differentiation. In: Cancer Research. 2004 ; Vol. 64, No. 13. pp. 4547-4554.
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abstract = "The t(8;21) chromosome abnormality in acute myeloid leukemia targets the AML1 and ETO genes to produce the leukemia fusion protein AML1-ETO. Another member of the ETO family, ETO-2/MTG16, is highly expressed in murine and human hematopoietic cells, bears >75{\%} homology to ETO, and like ETO, contains a conserved MYND domain that interacts with the nuclear receptor corepressor (N-CoR). AML1-ETO prevents granulocyte but not macrophage differentiation of murine 32Dcl3 granulocyte/macrophage progenitors. One possible mechanism is recruitment of N-CoR to aberrantly repress AML1 target genes. We wished to examine another mechanism by which AML1-ETO might impair granulocyte differentiation. We demonstrate that AML1-ETO decreases interactions between ETO-2 and N-CoR. Furthermore, overexpression of ETO-2 relieves AML1-ETO-induced granulocyte differentiation arrest. This suggests that decreased interactions between ETO-2 and N-CoR may contribute to granulocyte differentiation impairment. The MYND domain coimmunoprecipitates with N-CoR and inhibits interactions between ETO-2 and N-CoR, presumably by occupying the ETO-2 binding site on N-CoR. This inhibition of ETO-2 interactions with N-CoR is specific because the MYND domain does not inhibit retinoic acid receptor interactions with N-CoR. To examine the effect of decreasing interactions between ETO-2 and N-CoR in hematopoietic cells, without effects of AML1-ETO such as direct repression of AML1 target genes, the MYND domain was expressed in 32Dcl3 and human CD34+ cells. The MYND domain prevented granulocyte but not macrophage differentiation of both 32Dcl3 and human CD34+ cells, recapitulating this effect of AML1-ETO. In conclusion, decreasing interactions between ETO-2 and N-CoR, an effect of AML1-ETO, inhibits granulocyte differentiation.",
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AU - Ibañez, Vinzon

AU - Sharma, Arun

AU - Buonamici, Silvia

AU - Verma, Amit K.

AU - Kalakonda, Sudhakar

AU - Wang, Jianxiang

AU - Kadkol, ShriHari

AU - Saunthararajah, Yogen

PY - 2004/7/1

Y1 - 2004/7/1

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