Amino-terminal sequence of glycoprotein D of herpes simplex virus types 1 and 2

R. J. Eisenberg, D. Long, R. Hogue Angeletti, G. H. Cohen

Research output: Contribution to journalArticle

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Abstract

Glycoprotein D (gD) of herpes simplex virus is a structural component of the virion envelope which stimulates production of high titers of herpes simplex virus type-common neutralizing antibody. We carried out automated N-terminal amino acid sequencing studies on radiolabeled preparations of gD-1 (gD of herpes simplex virus type 1) and gD-2 (gD of herpes simplex virus type 2). Although some differences were noted, particularly in the methionine and alanine profiles for gD-1 and 9d-2, the amino acid sequence of a number of the first 30 residues of the amino terminus of gD-1 and gD-2 appears to be quite similar. For both proteins, the first residue is a lysine. When we compared our sequence data for gD-1 with those predicted by nucleic acid sequencing, the two sequences could be aligned (with one exception) starting at residue 26 (lysine) of the predicted sequence. Thus, the first 25 amino acids of the predicted sequence are absent from the polypeptides isolated from infected cells.

Original languageEnglish (US)
Pages (from-to)265-268
Number of pages4
JournalJournal of Virology
Volume49
Issue number1
StatePublished - 1984
Externally publishedYes

Fingerprint

Human herpesvirus 2
Human herpesvirus 1
Human Herpesvirus 2
Human Herpesvirus 1
glycoproteins
Glycoproteins
Simplexvirus
herpes simplex
Lysine
Amino Acid Sequence
lysine
Protein Sequence Analysis
glycoprotein D-herpes simplex virus type 2
Human herpesvirus 1 glycoprotein D
Neutralizing Antibodies
viruses
Alanine
Methionine
Virion
amino acids

ASJC Scopus subject areas

  • Immunology

Cite this

Eisenberg, R. J., Long, D., Hogue Angeletti, R., & Cohen, G. H. (1984). Amino-terminal sequence of glycoprotein D of herpes simplex virus types 1 and 2. Journal of Virology, 49(1), 265-268.

Amino-terminal sequence of glycoprotein D of herpes simplex virus types 1 and 2. / Eisenberg, R. J.; Long, D.; Hogue Angeletti, R.; Cohen, G. H.

In: Journal of Virology, Vol. 49, No. 1, 1984, p. 265-268.

Research output: Contribution to journalArticle

Eisenberg, RJ, Long, D, Hogue Angeletti, R & Cohen, GH 1984, 'Amino-terminal sequence of glycoprotein D of herpes simplex virus types 1 and 2', Journal of Virology, vol. 49, no. 1, pp. 265-268.
Eisenberg RJ, Long D, Hogue Angeletti R, Cohen GH. Amino-terminal sequence of glycoprotein D of herpes simplex virus types 1 and 2. Journal of Virology. 1984;49(1):265-268.
Eisenberg, R. J. ; Long, D. ; Hogue Angeletti, R. ; Cohen, G. H. / Amino-terminal sequence of glycoprotein D of herpes simplex virus types 1 and 2. In: Journal of Virology. 1984 ; Vol. 49, No. 1. pp. 265-268.
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