Amino Acid Residues That Are Critical for in Vivo Catalytic Activity of CtpA, the Carboxyl-terminal Processing Protease for the D1 Protein of Photosystem II

Noritoshi Inagaki, Radhashree Maitra, Kimiyuki Satoh, Himadri B. Pakrasi

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22 Citations (Scopus)

Abstract

CtpA, a carboxyl-terminal processing protease, is a member of a novel family of endoproteases that includes a tail-specific protease from Escherichia coli. In oxygenic photosynthetic organisms, CtpA catalyzes C-terminal processing of the D1 protein of photosystem II, an essential event for the assembly of a manganese cluster and consequent light-mediated water oxidation. We introduced site-specific mutations at 14 conserved residues of CtpA in the cyanobacterium Synechocystis sp. PCC 6803 to examine their functional roles. Analysis of the photoautotrophic growth capabilities of these mutants, their ability to process precursor D1 protein and hence evolve oxygen, along with an estimation of the protease content in the mutants revealed that five of these residues are critical for in vivo activity of CtpA. Recent x-ray crystal structure analysis of CtpA from the eukaryotic alga Scenedesmus obliquus (Liao, D.-I., Qian, J., Chisholm, D. A., Jordan, D. B. and Diner, B. A. (2000) Nat. Struct. Biol. 7, 749-753) has shown that the residues equivalent to Ser-313 and Lys-338, two of the five residues mentioned above, form the catalytic center of this enzyme. Our in vivo analysis demonstrates that the three other residues, Asp-253, Arg-255, and Glu-316, are also important determinants of the catalytic activity of CtpA.

Original languageEnglish (US)
Pages (from-to)30099-30105
Number of pages7
JournalJournal of Biological Chemistry
Volume276
Issue number32
DOIs
StatePublished - Aug 10 2001
Externally publishedYes

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Photosystem II Protein Complex
Catalyst activity
Peptide Hydrolases
Scenedesmus
Synechocystis
Amino Acids
Protein Precursors
Cyanobacteria
Algae
Manganese
Processing
Escherichia coli
Proteins
Crystal structure
X-Rays
Oxygen
Light
X rays
Oxidation
Mutation

ASJC Scopus subject areas

  • Biochemistry

Cite this

Amino Acid Residues That Are Critical for in Vivo Catalytic Activity of CtpA, the Carboxyl-terminal Processing Protease for the D1 Protein of Photosystem II. / Inagaki, Noritoshi; Maitra, Radhashree; Satoh, Kimiyuki; Pakrasi, Himadri B.

In: Journal of Biological Chemistry, Vol. 276, No. 32, 10.08.2001, p. 30099-30105.

Research output: Contribution to journalArticle

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