Alternative splicing in the pore-forming region of shaker potassium channels

Marshall Kim, Deborah J. Baro, Cathy C. Lanning, Mehul Doshi, Jeremy Farnham, Howard S. Moskowitz, Jack H. Peck, Baldomero M. Olivera, Ronald M. Harris-Warrick

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

We have cloned cDNAs for the shaker potassium channel gene from the spiny lobster Panulirus interruptus. As previously found in Drosophila, there is alternative splicing at the 5' and 3' ends of the coding region. However, in Panulirus shaker, alternative splicing also occurs within the pore- forming region of the protein. Three different splice variants were found within the P region, two of which bestow unique electrophysiological characteristics to channel function. Pore I and pore II variants differ in voltage dependence for activation, kinetics of inactivation, current rectification, and drug resistance. The pore 0 variant lacks a P region exon and does not produce a functional channel. This is the first example of alternative splicing within the pore-forming region of a voltage-dependent ion channel. We used a recently identified potassium channel blocker, κ- conotoxin PVIIA, to study the physiological role of the two pore forms. The toxin selectively blocked one pore form, whereas the other form, heteromers between the two pore forms, and Panulirus shal were not blocked. When it was tested in the Panulirus stomatogastric ganglion, the toxin produced no effects on transient K+ currents or synaptic transmission between neurons.

Original languageEnglish (US)
Pages (from-to)8213-8224
Number of pages12
JournalJournal of Neuroscience
Volume17
Issue number21
StatePublished - 1997
Externally publishedYes

Fingerprint

Shaker Superfamily of Potassium Channels
Palinuridae
Alternative Splicing
Conotoxins
Potassium Channel Blockers
Ion Channels
Drug Resistance
Synaptic Transmission
Ganglia
Drosophila
Exons
Complementary DNA
Neurons
Genes

Keywords

  • Alternative splicing
  • Conotoxin
  • Pore-forming region
  • Potassium channel
  • Shaker
  • Stomatogastric

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Kim, M., Baro, D. J., Lanning, C. C., Doshi, M., Farnham, J., Moskowitz, H. S., ... Harris-Warrick, R. M. (1997). Alternative splicing in the pore-forming region of shaker potassium channels. Journal of Neuroscience, 17(21), 8213-8224.

Alternative splicing in the pore-forming region of shaker potassium channels. / Kim, Marshall; Baro, Deborah J.; Lanning, Cathy C.; Doshi, Mehul; Farnham, Jeremy; Moskowitz, Howard S.; Peck, Jack H.; Olivera, Baldomero M.; Harris-Warrick, Ronald M.

In: Journal of Neuroscience, Vol. 17, No. 21, 1997, p. 8213-8224.

Research output: Contribution to journalArticle

Kim, M, Baro, DJ, Lanning, CC, Doshi, M, Farnham, J, Moskowitz, HS, Peck, JH, Olivera, BM & Harris-Warrick, RM 1997, 'Alternative splicing in the pore-forming region of shaker potassium channels', Journal of Neuroscience, vol. 17, no. 21, pp. 8213-8224.
Kim M, Baro DJ, Lanning CC, Doshi M, Farnham J, Moskowitz HS et al. Alternative splicing in the pore-forming region of shaker potassium channels. Journal of Neuroscience. 1997;17(21):8213-8224.
Kim, Marshall ; Baro, Deborah J. ; Lanning, Cathy C. ; Doshi, Mehul ; Farnham, Jeremy ; Moskowitz, Howard S. ; Peck, Jack H. ; Olivera, Baldomero M. ; Harris-Warrick, Ronald M. / Alternative splicing in the pore-forming region of shaker potassium channels. In: Journal of Neuroscience. 1997 ; Vol. 17, No. 21. pp. 8213-8224.
@article{666ef8cb21054aba8f967b57b2c1e1ae,
title = "Alternative splicing in the pore-forming region of shaker potassium channels",
abstract = "We have cloned cDNAs for the shaker potassium channel gene from the spiny lobster Panulirus interruptus. As previously found in Drosophila, there is alternative splicing at the 5' and 3' ends of the coding region. However, in Panulirus shaker, alternative splicing also occurs within the pore- forming region of the protein. Three different splice variants were found within the P region, two of which bestow unique electrophysiological characteristics to channel function. Pore I and pore II variants differ in voltage dependence for activation, kinetics of inactivation, current rectification, and drug resistance. The pore 0 variant lacks a P region exon and does not produce a functional channel. This is the first example of alternative splicing within the pore-forming region of a voltage-dependent ion channel. We used a recently identified potassium channel blocker, κ- conotoxin PVIIA, to study the physiological role of the two pore forms. The toxin selectively blocked one pore form, whereas the other form, heteromers between the two pore forms, and Panulirus shal were not blocked. When it was tested in the Panulirus stomatogastric ganglion, the toxin produced no effects on transient K+ currents or synaptic transmission between neurons.",
keywords = "Alternative splicing, Conotoxin, Pore-forming region, Potassium channel, Shaker, Stomatogastric",
author = "Marshall Kim and Baro, {Deborah J.} and Lanning, {Cathy C.} and Mehul Doshi and Jeremy Farnham and Moskowitz, {Howard S.} and Peck, {Jack H.} and Olivera, {Baldomero M.} and Harris-Warrick, {Ronald M.}",
year = "1997",
language = "English (US)",
volume = "17",
pages = "8213--8224",
journal = "Journal of Neuroscience",
issn = "0270-6474",
publisher = "Society for Neuroscience",
number = "21",

}

TY - JOUR

T1 - Alternative splicing in the pore-forming region of shaker potassium channels

AU - Kim, Marshall

AU - Baro, Deborah J.

AU - Lanning, Cathy C.

AU - Doshi, Mehul

AU - Farnham, Jeremy

AU - Moskowitz, Howard S.

AU - Peck, Jack H.

AU - Olivera, Baldomero M.

AU - Harris-Warrick, Ronald M.

PY - 1997

Y1 - 1997

N2 - We have cloned cDNAs for the shaker potassium channel gene from the spiny lobster Panulirus interruptus. As previously found in Drosophila, there is alternative splicing at the 5' and 3' ends of the coding region. However, in Panulirus shaker, alternative splicing also occurs within the pore- forming region of the protein. Three different splice variants were found within the P region, two of which bestow unique electrophysiological characteristics to channel function. Pore I and pore II variants differ in voltage dependence for activation, kinetics of inactivation, current rectification, and drug resistance. The pore 0 variant lacks a P region exon and does not produce a functional channel. This is the first example of alternative splicing within the pore-forming region of a voltage-dependent ion channel. We used a recently identified potassium channel blocker, κ- conotoxin PVIIA, to study the physiological role of the two pore forms. The toxin selectively blocked one pore form, whereas the other form, heteromers between the two pore forms, and Panulirus shal were not blocked. When it was tested in the Panulirus stomatogastric ganglion, the toxin produced no effects on transient K+ currents or synaptic transmission between neurons.

AB - We have cloned cDNAs for the shaker potassium channel gene from the spiny lobster Panulirus interruptus. As previously found in Drosophila, there is alternative splicing at the 5' and 3' ends of the coding region. However, in Panulirus shaker, alternative splicing also occurs within the pore- forming region of the protein. Three different splice variants were found within the P region, two of which bestow unique electrophysiological characteristics to channel function. Pore I and pore II variants differ in voltage dependence for activation, kinetics of inactivation, current rectification, and drug resistance. The pore 0 variant lacks a P region exon and does not produce a functional channel. This is the first example of alternative splicing within the pore-forming region of a voltage-dependent ion channel. We used a recently identified potassium channel blocker, κ- conotoxin PVIIA, to study the physiological role of the two pore forms. The toxin selectively blocked one pore form, whereas the other form, heteromers between the two pore forms, and Panulirus shal were not blocked. When it was tested in the Panulirus stomatogastric ganglion, the toxin produced no effects on transient K+ currents or synaptic transmission between neurons.

KW - Alternative splicing

KW - Conotoxin

KW - Pore-forming region

KW - Potassium channel

KW - Shaker

KW - Stomatogastric

UR - http://www.scopus.com/inward/record.url?scp=0030773751&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030773751&partnerID=8YFLogxK

M3 - Article

VL - 17

SP - 8213

EP - 8224

JO - Journal of Neuroscience

JF - Journal of Neuroscience

SN - 0270-6474

IS - 21

ER -