Altered cellular interactions between endothelial cells and nonenzymatically glucosylated laminin/type IV collagen

C. S. Haitoglou, E. C. Tsilibary, M. Brownlee, A. S. Charonis

Research output: Contribution to journalArticle

169 Scopus citations

Abstract

Laminin and type IV collagen are two major basement membrane glycoproteins. In previous studies it has been shown that nonenzymatic glucosylation induces structural alterations of these macromolecules and also reduces their ability to self-associate. In the present study, endothelial cells were tested for their ability to adhere and spread on nonenzymatically glucosylated laminin and type IV collagen. Adhesion and spreading were reduced when glucosylated macromolecules were used as substrates. Glucosylation-induced changes in adhesion and spreading may be an important initial event signaling other phenotypic modifications of cells in the microvasculature and may be a crucial factor in order to understand the pathogenesis of diabetic microangiopathy at the molecular level.

Original languageEnglish (US)
Pages (from-to)12404-12407
Number of pages4
JournalJournal of Biological Chemistry
Volume267
Issue number18
StatePublished - Jan 1 1992

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Haitoglou, C. S., Tsilibary, E. C., Brownlee, M., & Charonis, A. S. (1992). Altered cellular interactions between endothelial cells and nonenzymatically glucosylated laminin/type IV collagen. Journal of Biological Chemistry, 267(18), 12404-12407.