Altered cellular interactions between endothelial cells and nonenzymatically glucosylated laminin/type IV collagen

Costas S. Haitoglou, Effie C. Tsilibary, Michael Brownlee, Aristidis S. Charonis

Research output: Contribution to journalArticle

168 Citations (Scopus)

Abstract

Laminin and type IV collagen are two major basement membrane glycoproteins. In previous studies it has been shown that nonenzymatic glucosylation induces structural alterations of these macromolecules and also reduces their ability to self-associate. In the present study, endothelial cells were tested for their ability to adhere and spread on nonenzymatically glucosylated laminin and type IV collagen. Adhesion and spreading were reduced when glucosylated macromolecules were used as substrates. Glucosylation-induced changes in adhesion and spreading may be an important initial event signaling other phenotypic modifications of cells in the microvasculature and may be a crucial factor in order to understand the pathogenesis of diabetic microangiopathy at the molecular level.

Original languageEnglish (US)
Pages (from-to)12404-12407
Number of pages4
JournalJournal of Biological Chemistry
Volume267
Issue number18
StatePublished - Jun 25 1992

Fingerprint

Collagen Type IV
Endothelial cells
Laminin
Macromolecules
Adhesion
Endothelial Cells
Diabetic Angiopathies
Membrane Glycoproteins
Microvessels
Basement Membrane
Substrates

ASJC Scopus subject areas

  • Biochemistry

Cite this

Haitoglou, C. S., Tsilibary, E. C., Brownlee, M., & Charonis, A. S. (1992). Altered cellular interactions between endothelial cells and nonenzymatically glucosylated laminin/type IV collagen. Journal of Biological Chemistry, 267(18), 12404-12407.

Altered cellular interactions between endothelial cells and nonenzymatically glucosylated laminin/type IV collagen. / Haitoglou, Costas S.; Tsilibary, Effie C.; Brownlee, Michael; Charonis, Aristidis S.

In: Journal of Biological Chemistry, Vol. 267, No. 18, 25.06.1992, p. 12404-12407.

Research output: Contribution to journalArticle

Haitoglou, CS, Tsilibary, EC, Brownlee, M & Charonis, AS 1992, 'Altered cellular interactions between endothelial cells and nonenzymatically glucosylated laminin/type IV collagen', Journal of Biological Chemistry, vol. 267, no. 18, pp. 12404-12407.
Haitoglou, Costas S. ; Tsilibary, Effie C. ; Brownlee, Michael ; Charonis, Aristidis S. / Altered cellular interactions between endothelial cells and nonenzymatically glucosylated laminin/type IV collagen. In: Journal of Biological Chemistry. 1992 ; Vol. 267, No. 18. pp. 12404-12407.
@article{c02497aa180a42669f436c9bdf52a6d7,
title = "Altered cellular interactions between endothelial cells and nonenzymatically glucosylated laminin/type IV collagen",
abstract = "Laminin and type IV collagen are two major basement membrane glycoproteins. In previous studies it has been shown that nonenzymatic glucosylation induces structural alterations of these macromolecules and also reduces their ability to self-associate. In the present study, endothelial cells were tested for their ability to adhere and spread on nonenzymatically glucosylated laminin and type IV collagen. Adhesion and spreading were reduced when glucosylated macromolecules were used as substrates. Glucosylation-induced changes in adhesion and spreading may be an important initial event signaling other phenotypic modifications of cells in the microvasculature and may be a crucial factor in order to understand the pathogenesis of diabetic microangiopathy at the molecular level.",
author = "Haitoglou, {Costas S.} and Tsilibary, {Effie C.} and Michael Brownlee and Charonis, {Aristidis S.}",
year = "1992",
month = "6",
day = "25",
language = "English (US)",
volume = "267",
pages = "12404--12407",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "18",

}

TY - JOUR

T1 - Altered cellular interactions between endothelial cells and nonenzymatically glucosylated laminin/type IV collagen

AU - Haitoglou, Costas S.

AU - Tsilibary, Effie C.

AU - Brownlee, Michael

AU - Charonis, Aristidis S.

PY - 1992/6/25

Y1 - 1992/6/25

N2 - Laminin and type IV collagen are two major basement membrane glycoproteins. In previous studies it has been shown that nonenzymatic glucosylation induces structural alterations of these macromolecules and also reduces their ability to self-associate. In the present study, endothelial cells were tested for their ability to adhere and spread on nonenzymatically glucosylated laminin and type IV collagen. Adhesion and spreading were reduced when glucosylated macromolecules were used as substrates. Glucosylation-induced changes in adhesion and spreading may be an important initial event signaling other phenotypic modifications of cells in the microvasculature and may be a crucial factor in order to understand the pathogenesis of diabetic microangiopathy at the molecular level.

AB - Laminin and type IV collagen are two major basement membrane glycoproteins. In previous studies it has been shown that nonenzymatic glucosylation induces structural alterations of these macromolecules and also reduces their ability to self-associate. In the present study, endothelial cells were tested for their ability to adhere and spread on nonenzymatically glucosylated laminin and type IV collagen. Adhesion and spreading were reduced when glucosylated macromolecules were used as substrates. Glucosylation-induced changes in adhesion and spreading may be an important initial event signaling other phenotypic modifications of cells in the microvasculature and may be a crucial factor in order to understand the pathogenesis of diabetic microangiopathy at the molecular level.

UR - http://www.scopus.com/inward/record.url?scp=0026696647&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026696647&partnerID=8YFLogxK

M3 - Article

C2 - 1618745

AN - SCOPUS:0026696647

VL - 267

SP - 12404

EP - 12407

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 18

ER -