Abstract
We previously reported decreased lymphocyte proliferative responses among older women with persistent human papillomavirus (HPV) infection. To characterize the phenotype of peripheral lymphocytes associated with persistent HPV infection, we evaluated the expression of different cell surface markers in peripheral blood mononuclear cells (PBMCs) from a case-control study within a 10,049 woman population-based cohort study in Guanacaste, Costa Rica. Women in the cohort aged 46-74 and with HPV results at their 5th year anniversary visit were considered, and all women (n = 87) with persistent HPV infections, all women (n = 196) with transient HPV infections and a random sample of HPV DNA-negative women (n = 261) frequency-matched to cases on age were selected for this study. A median of 3 years after the case-control matching visit, cervical cells were collected for liquid-based cytology and repeat HPV DNA genotyping. Blood was obtained from which PBMCs were extracted and cryopreserved for immunological phenotyping via flow cytometry. Significant increases in risk of HPV persistence were observed for 3 marker subsets indicative of immune cell activation/differentiation. Relative risk estimates were 5.4 (95% CI = 2.2-13.3) for CD69+CD4+, 2.6 (95% CI = 1.2-5.9) for HLADR +CD3+CD4+ and 2.3 (95% CI = 1.1-4.7) for CD45RO+CD27-CD8+. A significant decrease in HPV persistence was observed for a subset marker indicative of an immature, undifferentiated memory state CD45RO+CD27+CD4+ (OR = 0.36; 95% CI = 0.17-0.76). Adjustment for these markers only partially explained the previously reported association between decreased lymphoproliferative responses and persistent HPV infection. Whether phenotypic alterations observed predispose to HPV persistence or result from it should be the focus of future studies.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 597-607 |
| Number of pages | 11 |
| Journal | International Journal of Cancer |
| Volume | 128 |
| Issue number | 3 |
| DOIs | |
| State | Published - Feb 1 2011 |
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Keywords
- HPV persistent infection
- older women
- T-cell activation and differentiation
- T-cell distribution
ASJC Scopus subject areas
- Cancer Research
- Oncology
Cite this
Alterations of T-cell surface markers in older women with persistent human papillomavirus infection. / Rodríguez, Ana Cecilia; García-Piñeres, Alfonso J.; Hildesheim, Allan; Herrero, Rolando; Trivett, Matthew; Williams, Marcus; Atmella, Ivannia; Ramírez, Margarita; Villegas, Maricela; Schiffman, Mark; Burk, Robert D.; Freer, Enrique; Bonilla, José; Bratti, Concepción; Pinto, Ligia A.
In: International Journal of Cancer, Vol. 128, No. 3, 01.02.2011, p. 597-607.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Alterations of T-cell surface markers in older women with persistent human papillomavirus infection
AU - Rodríguez, Ana Cecilia
AU - García-Piñeres, Alfonso J.
AU - Hildesheim, Allan
AU - Herrero, Rolando
AU - Trivett, Matthew
AU - Williams, Marcus
AU - Atmella, Ivannia
AU - Ramírez, Margarita
AU - Villegas, Maricela
AU - Schiffman, Mark
AU - Burk, Robert D.
AU - Freer, Enrique
AU - Bonilla, José
AU - Bratti, Concepción
AU - Pinto, Ligia A.
PY - 2011/2/1
Y1 - 2011/2/1
N2 - We previously reported decreased lymphocyte proliferative responses among older women with persistent human papillomavirus (HPV) infection. To characterize the phenotype of peripheral lymphocytes associated with persistent HPV infection, we evaluated the expression of different cell surface markers in peripheral blood mononuclear cells (PBMCs) from a case-control study within a 10,049 woman population-based cohort study in Guanacaste, Costa Rica. Women in the cohort aged 46-74 and with HPV results at their 5th year anniversary visit were considered, and all women (n = 87) with persistent HPV infections, all women (n = 196) with transient HPV infections and a random sample of HPV DNA-negative women (n = 261) frequency-matched to cases on age were selected for this study. A median of 3 years after the case-control matching visit, cervical cells were collected for liquid-based cytology and repeat HPV DNA genotyping. Blood was obtained from which PBMCs were extracted and cryopreserved for immunological phenotyping via flow cytometry. Significant increases in risk of HPV persistence were observed for 3 marker subsets indicative of immune cell activation/differentiation. Relative risk estimates were 5.4 (95% CI = 2.2-13.3) for CD69+CD4+, 2.6 (95% CI = 1.2-5.9) for HLADR +CD3+CD4+ and 2.3 (95% CI = 1.1-4.7) for CD45RO+CD27-CD8+. A significant decrease in HPV persistence was observed for a subset marker indicative of an immature, undifferentiated memory state CD45RO+CD27+CD4+ (OR = 0.36; 95% CI = 0.17-0.76). Adjustment for these markers only partially explained the previously reported association between decreased lymphoproliferative responses and persistent HPV infection. Whether phenotypic alterations observed predispose to HPV persistence or result from it should be the focus of future studies.
AB - We previously reported decreased lymphocyte proliferative responses among older women with persistent human papillomavirus (HPV) infection. To characterize the phenotype of peripheral lymphocytes associated with persistent HPV infection, we evaluated the expression of different cell surface markers in peripheral blood mononuclear cells (PBMCs) from a case-control study within a 10,049 woman population-based cohort study in Guanacaste, Costa Rica. Women in the cohort aged 46-74 and with HPV results at their 5th year anniversary visit were considered, and all women (n = 87) with persistent HPV infections, all women (n = 196) with transient HPV infections and a random sample of HPV DNA-negative women (n = 261) frequency-matched to cases on age were selected for this study. A median of 3 years after the case-control matching visit, cervical cells were collected for liquid-based cytology and repeat HPV DNA genotyping. Blood was obtained from which PBMCs were extracted and cryopreserved for immunological phenotyping via flow cytometry. Significant increases in risk of HPV persistence were observed for 3 marker subsets indicative of immune cell activation/differentiation. Relative risk estimates were 5.4 (95% CI = 2.2-13.3) for CD69+CD4+, 2.6 (95% CI = 1.2-5.9) for HLADR +CD3+CD4+ and 2.3 (95% CI = 1.1-4.7) for CD45RO+CD27-CD8+. A significant decrease in HPV persistence was observed for a subset marker indicative of an immature, undifferentiated memory state CD45RO+CD27+CD4+ (OR = 0.36; 95% CI = 0.17-0.76). Adjustment for these markers only partially explained the previously reported association between decreased lymphoproliferative responses and persistent HPV infection. Whether phenotypic alterations observed predispose to HPV persistence or result from it should be the focus of future studies.
KW - HPV persistent infection
KW - older women
KW - T-cell activation and differentiation
KW - T-cell distribution
UR - http://www.scopus.com/inward/record.url?scp=78649824119&partnerID=8YFLogxK
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U2 - 10.1002/ijc.25371
DO - 10.1002/ijc.25371
M3 - Article
VL - 128
SP - 597
EP - 607
JO - International Journal of Cancer
JF - International Journal of Cancer
SN - 0020-7136
IS - 3
ER -