Alterations of T-cell surface markers in older women with persistent human papillomavirus infection

Ana Cecilia Rodríguez, Alfonso J. García-Piñeres, Allan Hildesheim, Rolando Herrero, Matthew Trivett, Marcus Williams, Ivannia Atmella, Margarita Ramírez, Maricela Villegas, Mark Schiffman, Robert D. Burk, Enrique Freer, José Bonilla, Concepción Bratti, Ligia A. Pinto

Research output: Contribution to journalArticle

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Abstract

We previously reported decreased lymphocyte proliferative responses among older women with persistent human papillomavirus (HPV) infection. To characterize the phenotype of peripheral lymphocytes associated with persistent HPV infection, we evaluated the expression of different cell surface markers in peripheral blood mononuclear cells (PBMCs) from a case-control study within a 10,049 woman population-based cohort study in Guanacaste, Costa Rica. Women in the cohort aged 46-74 and with HPV results at their 5th year anniversary visit were considered, and all women (n = 87) with persistent HPV infections, all women (n = 196) with transient HPV infections and a random sample of HPV DNA-negative women (n = 261) frequency-matched to cases on age were selected for this study. A median of 3 years after the case-control matching visit, cervical cells were collected for liquid-based cytology and repeat HPV DNA genotyping. Blood was obtained from which PBMCs were extracted and cryopreserved for immunological phenotyping via flow cytometry. Significant increases in risk of HPV persistence were observed for 3 marker subsets indicative of immune cell activation/differentiation. Relative risk estimates were 5.4 (95% CI = 2.2-13.3) for CD69+CD4+, 2.6 (95% CI = 1.2-5.9) for HLADR +CD3+CD4+ and 2.3 (95% CI = 1.1-4.7) for CD45RO+CD27-CD8+. A significant decrease in HPV persistence was observed for a subset marker indicative of an immature, undifferentiated memory state CD45RO+CD27+CD4+ (OR = 0.36; 95% CI = 0.17-0.76). Adjustment for these markers only partially explained the previously reported association between decreased lymphoproliferative responses and persistent HPV infection. Whether phenotypic alterations observed predispose to HPV persistence or result from it should be the focus of future studies.

Original languageEnglish (US)
Pages (from-to)597-607
Number of pages11
JournalInternational Journal of Cancer
Volume128
Issue number3
DOIs
StatePublished - Feb 1 2011

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Papillomavirus Infections
T-Lymphocytes
Blood Cells
Lymphocytes
Costa Rica
DNA
Anniversaries and Special Events
Cell Biology
Case-Control Studies
Cell Differentiation
Flow Cytometry
Cohort Studies
Phenotype

Keywords

  • HPV persistent infection
  • older women
  • T-cell activation and differentiation
  • T-cell distribution

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Rodríguez, A. C., García-Piñeres, A. J., Hildesheim, A., Herrero, R., Trivett, M., Williams, M., ... Pinto, L. A. (2011). Alterations of T-cell surface markers in older women with persistent human papillomavirus infection. International Journal of Cancer, 128(3), 597-607. https://doi.org/10.1002/ijc.25371

Alterations of T-cell surface markers in older women with persistent human papillomavirus infection. / Rodríguez, Ana Cecilia; García-Piñeres, Alfonso J.; Hildesheim, Allan; Herrero, Rolando; Trivett, Matthew; Williams, Marcus; Atmella, Ivannia; Ramírez, Margarita; Villegas, Maricela; Schiffman, Mark; Burk, Robert D.; Freer, Enrique; Bonilla, José; Bratti, Concepción; Pinto, Ligia A.

In: International Journal of Cancer, Vol. 128, No. 3, 01.02.2011, p. 597-607.

Research output: Contribution to journalArticle

Rodríguez, AC, García-Piñeres, AJ, Hildesheim, A, Herrero, R, Trivett, M, Williams, M, Atmella, I, Ramírez, M, Villegas, M, Schiffman, M, Burk, RD, Freer, E, Bonilla, J, Bratti, C & Pinto, LA 2011, 'Alterations of T-cell surface markers in older women with persistent human papillomavirus infection', International Journal of Cancer, vol. 128, no. 3, pp. 597-607. https://doi.org/10.1002/ijc.25371
Rodríguez AC, García-Piñeres AJ, Hildesheim A, Herrero R, Trivett M, Williams M et al. Alterations of T-cell surface markers in older women with persistent human papillomavirus infection. International Journal of Cancer. 2011 Feb 1;128(3):597-607. https://doi.org/10.1002/ijc.25371
Rodríguez, Ana Cecilia ; García-Piñeres, Alfonso J. ; Hildesheim, Allan ; Herrero, Rolando ; Trivett, Matthew ; Williams, Marcus ; Atmella, Ivannia ; Ramírez, Margarita ; Villegas, Maricela ; Schiffman, Mark ; Burk, Robert D. ; Freer, Enrique ; Bonilla, José ; Bratti, Concepción ; Pinto, Ligia A. / Alterations of T-cell surface markers in older women with persistent human papillomavirus infection. In: International Journal of Cancer. 2011 ; Vol. 128, No. 3. pp. 597-607.
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