Alterations in CSF-1 receptor expression and protein tyrosine phosphorylation in autonomous mutants of a CSF-1 dependent macrophage cell line

Peksio Dello Sbarba, Jeffrey W. Pollard, E. Richard Stanley

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

One approach to the problem of growth factor signal transduction is to identify alterations in growth factor-regulated events in mutants possessing an altered proliferative response. This approach was used in a study of the alterations in protein tyrosine phosphorylation in 22 independently-arising autonomous mutants of the CSF-1-dependent mouse macrophage cell line, BACl.2F5. Only 4 of the mutants produced CSF-1 and/or factors that were capable of down-regulating the CSF-1 receptor (CSF-1R), suggesting that the majority were altered in the signal tranduction pathway for proliferation. All of the mutants possessed lower numbers (13-89% of cell surface CSF-1Rs than wild type cells. With two possible exceptions, the phosphorylation of the anti-phosphotyrosine-reactive cell surface CSF-1 Rs from CSF-1 stimulated cells was directly proportional to cell surface CSF-I R number. In the absence of CSF-I, three of the 22 mutants exhibited tyrosine phosphorylation of proteins that was not observed in wild type cells under the same conditions. The results indicate that this approach will be useful in the analysis of the growth factor regulated pathway for cell proliferation.

Original languageEnglish (US)
Pages (from-to)75-85
Number of pages11
JournalGrowth Factors
Volume5
Issue number1
DOIs
StatePublished - 1991

Fingerprint

Macrophage Colony-Stimulating Factor Receptors
Phosphorylation
Macrophage Colony-Stimulating Factor
Macrophages
Tyrosine
Cells
Cell Line
Intercellular Signaling Peptides and Proteins
Proteins
Signal transduction
Phosphotyrosine
Signal Transduction
Cell proliferation
Cell Proliferation

Keywords

  • Autonomous cell growth
  • CSF-1 receptor
  • Growth factor independence
  • Mutant cell lines
  • Tyrosine phosphorylation

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Endocrinology
  • Cell Biology

Cite this

Alterations in CSF-1 receptor expression and protein tyrosine phosphorylation in autonomous mutants of a CSF-1 dependent macrophage cell line. / Sbarba, Peksio Dello; Pollard, Jeffrey W.; Stanley, E. Richard.

In: Growth Factors, Vol. 5, No. 1, 1991, p. 75-85.

Research output: Contribution to journalArticle

Sbarba, Peksio Dello ; Pollard, Jeffrey W. ; Stanley, E. Richard. / Alterations in CSF-1 receptor expression and protein tyrosine phosphorylation in autonomous mutants of a CSF-1 dependent macrophage cell line. In: Growth Factors. 1991 ; Vol. 5, No. 1. pp. 75-85.
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