Akt inhibitor MK2206 prevents influenza pH1N1 virus infection in vitro

Oxana V. Denisova, Sandra Sod̈erholm, Salla Virtanen, Carina Von Schantz, Dmitrii Bychkov, Elena Vashchinkina, Jens Desloovere, Janne Tynell, Niina Ikonen, Linda L. Theisen, Tuula A. Nyman, Sampsa Matikainen, Olli Kallioniemi, Ilkka Julkunen, Claude P. Muller, Xavier Saelens, Vladislav Verkhusha, Denis E. Kainov

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The influenza pH1N1 virus caused a global flu pandemic in 2009 and continues manifestation as a seasonal virus. Better understanding of the virus-host cell interaction could result in development of better prevention and treatment options. Here we show that the Akt inhibitor MK2206 blocks influenza pH1N1 virus infection in vitro. In particular, at noncytotoxic concentrations, MK2206 alters Akt signaling and inhibits endocytic uptake of the virus. Interestingly, MK2206 is unable to inhibit H3N2, H7N9, and H5N1 viruses, indicating that pH1N1 evolved specific requirements for efficient infection. Thus, Akt signaling could be exploited further for development of better therapeutics against pH1N1 virus.

Original languageEnglish (US)
Pages (from-to)3689-3696
Number of pages8
JournalAntimicrobial Agents and Chemotherapy
Volume58
Issue number7
DOIs
StatePublished - 2014

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Virus Diseases
Orthomyxoviridae
Viruses
H7N9 Subtype Influenza A Virus
H3N2 Subtype Influenza A Virus
H5N1 Subtype Influenza A Virus
Pandemics
Cell Communication
In Vitro Techniques
MK 2206
Infection
Therapeutics

ASJC Scopus subject areas

  • Pharmacology
  • Infectious Diseases
  • Pharmacology (medical)

Cite this

Denisova, O. V., Sod̈erholm, S., Virtanen, S., Von Schantz, C., Bychkov, D., Vashchinkina, E., ... Kainov, D. E. (2014). Akt inhibitor MK2206 prevents influenza pH1N1 virus infection in vitro. Antimicrobial Agents and Chemotherapy, 58(7), 3689-3696. https://doi.org/10.1128/AAC.02798-13

Akt inhibitor MK2206 prevents influenza pH1N1 virus infection in vitro. / Denisova, Oxana V.; Sod̈erholm, Sandra; Virtanen, Salla; Von Schantz, Carina; Bychkov, Dmitrii; Vashchinkina, Elena; Desloovere, Jens; Tynell, Janne; Ikonen, Niina; Theisen, Linda L.; Nyman, Tuula A.; Matikainen, Sampsa; Kallioniemi, Olli; Julkunen, Ilkka; Muller, Claude P.; Saelens, Xavier; Verkhusha, Vladislav; Kainov, Denis E.

In: Antimicrobial Agents and Chemotherapy, Vol. 58, No. 7, 2014, p. 3689-3696.

Research output: Contribution to journalArticle

Denisova, OV, Sod̈erholm, S, Virtanen, S, Von Schantz, C, Bychkov, D, Vashchinkina, E, Desloovere, J, Tynell, J, Ikonen, N, Theisen, LL, Nyman, TA, Matikainen, S, Kallioniemi, O, Julkunen, I, Muller, CP, Saelens, X, Verkhusha, V & Kainov, DE 2014, 'Akt inhibitor MK2206 prevents influenza pH1N1 virus infection in vitro', Antimicrobial Agents and Chemotherapy, vol. 58, no. 7, pp. 3689-3696. https://doi.org/10.1128/AAC.02798-13
Denisova OV, Sod̈erholm S, Virtanen S, Von Schantz C, Bychkov D, Vashchinkina E et al. Akt inhibitor MK2206 prevents influenza pH1N1 virus infection in vitro. Antimicrobial Agents and Chemotherapy. 2014;58(7):3689-3696. https://doi.org/10.1128/AAC.02798-13
Denisova, Oxana V. ; Sod̈erholm, Sandra ; Virtanen, Salla ; Von Schantz, Carina ; Bychkov, Dmitrii ; Vashchinkina, Elena ; Desloovere, Jens ; Tynell, Janne ; Ikonen, Niina ; Theisen, Linda L. ; Nyman, Tuula A. ; Matikainen, Sampsa ; Kallioniemi, Olli ; Julkunen, Ilkka ; Muller, Claude P. ; Saelens, Xavier ; Verkhusha, Vladislav ; Kainov, Denis E. / Akt inhibitor MK2206 prevents influenza pH1N1 virus infection in vitro. In: Antimicrobial Agents and Chemotherapy. 2014 ; Vol. 58, No. 7. pp. 3689-3696.
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