Aging promotes the development of diet-induced murine steatohepatitis but not steatosis

Luis Fontana, Enpeng Zhao, Muhammad Amir, Hanqing Dong, Kathryn E. Tanaka, Mark J. Czaja

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The prevalence of the metabolic syndrome and nonalcoholic fatty liver disease (NAFLD) in humans increases with age. It is unknown whether this association is secondary to the increased incidence of risk factors for NAFLD that occurs with aging, reflects the culmination of years of exposure to lifestyle factors such as a high-fat diet (HFD), or results from physiological changes that characterize aging. To examine this question, the development of NAFLD in response to a fixed period of HFD feeding was examined in mice of different ages. Mice aged 2, 8, and 18 months were fed 16 weeks of a low-fat diet or HFD. Increased body mass and insulin insensitivity occurred in response to HFD feeding irrespective of the age of the mice. The amount of HFD-induced hepatic steatosis as determined biochemically and histologically was also equivalent among the three ages. Liver injury occurred exclusively in the two older ages as reflected by increased serum alanine aminotransferase levels, positive terminal deoxynucleotide transferase-mediated deoxyuridine triphosphate nick end-labeling, and caspase activation. Older mice also had an elevated innate immune response with a more pronounced polarization of liver and adipose tissue macrophages into an M1 phenotype. Studies of cultured hepatocytes from young and old mice revealed that aged cells were selectively sensitized to the Fas death pathway. Conclusion: Aging does not promote the development of hepatic steatosis but leads to increased hepatocellular injury and inflammation that may be due in part to sensitization to the Fas death pathway and increased M1 macrophage polarization.

Original languageEnglish (US)
Pages (from-to)995-1004
Number of pages10
JournalHepatology
Volume57
Issue number3
DOIs
StatePublished - Mar 2013

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High Fat Diet
Fatty Liver
Diet
Liver
Macrophages
Fat-Restricted Diet
Wounds and Injuries
Caspases
Transferases
Alanine Transaminase
Innate Immunity
Adipose Tissue
Life Style
Hepatocytes
Insulin
Inflammation
Phenotype
Incidence
Serum
Non-alcoholic Fatty Liver Disease

ASJC Scopus subject areas

  • Hepatology

Cite this

Aging promotes the development of diet-induced murine steatohepatitis but not steatosis. / Fontana, Luis; Zhao, Enpeng; Amir, Muhammad; Dong, Hanqing; Tanaka, Kathryn E.; Czaja, Mark J.

In: Hepatology, Vol. 57, No. 3, 03.2013, p. 995-1004.

Research output: Contribution to journalArticle

Fontana, Luis ; Zhao, Enpeng ; Amir, Muhammad ; Dong, Hanqing ; Tanaka, Kathryn E. ; Czaja, Mark J. / Aging promotes the development of diet-induced murine steatohepatitis but not steatosis. In: Hepatology. 2013 ; Vol. 57, No. 3. pp. 995-1004.
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