Abstract
The MaxiK channel plays a critical role in the regulation of corporal smooth muscle tone and thereby erectile function. Given that ageing results in a decline in erectile function, we determined changes in the expression of MaxiK, which might impact erectile function. Quantitative-polymerase chain reaction demonstrated that although there is no significant change in transcription of the α- and β-subunits that comprise the MaxiK channel, there are significant changes in the expression of transcripts encoding different splice variants. One transcript, SV1, is 13-fold increased in expression in the ageing rat corpora. SV1 has previously been reported to trap other isoforms of the MaxiK channel in the cytoplasm. Correlating with increased expression of SV1, we observed in older rats there is approximately a 13-fold decrease in MaxiK protein in the corpora cell membrane and a greater proportion is retained in the cytoplasm (approximately threefold). These experiments demonstrate that ageing of the corpora is accompanied by changes in alternative splicing and cellular localization of the MaxiK channel.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 371-377 |
| Number of pages | 7 |
| Journal | International Journal of Impotence Research |
| Volume | 19 |
| Issue number | 4 |
| DOIs | |
| State | Published - Jul 2007 |
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Keywords
- Ageing
- Erectile dysfunction
- MaxiK channel
- Smooth muscle
- Splicing
ASJC Scopus subject areas
- Urology
Cite this
Ageing causes cytoplasmic retention of MaxiK channels in rat corporal smooth muscle cells. / Davies, Kelvin; Stanevsky, Y.; Tar, Moses T.; Chang, J. S.; Chance, M. R.; Melman, A.
In: International Journal of Impotence Research, Vol. 19, No. 4, 07.2007, p. 371-377.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Ageing causes cytoplasmic retention of MaxiK channels in rat corporal smooth muscle cells
AU - Davies, Kelvin
AU - Stanevsky, Y.
AU - Tar, Moses T.
AU - Chang, J. S.
AU - Chance, M. R.
AU - Melman, A.
PY - 2007/7
Y1 - 2007/7
N2 - The MaxiK channel plays a critical role in the regulation of corporal smooth muscle tone and thereby erectile function. Given that ageing results in a decline in erectile function, we determined changes in the expression of MaxiK, which might impact erectile function. Quantitative-polymerase chain reaction demonstrated that although there is no significant change in transcription of the α- and β-subunits that comprise the MaxiK channel, there are significant changes in the expression of transcripts encoding different splice variants. One transcript, SV1, is 13-fold increased in expression in the ageing rat corpora. SV1 has previously been reported to trap other isoforms of the MaxiK channel in the cytoplasm. Correlating with increased expression of SV1, we observed in older rats there is approximately a 13-fold decrease in MaxiK protein in the corpora cell membrane and a greater proportion is retained in the cytoplasm (approximately threefold). These experiments demonstrate that ageing of the corpora is accompanied by changes in alternative splicing and cellular localization of the MaxiK channel.
AB - The MaxiK channel plays a critical role in the regulation of corporal smooth muscle tone and thereby erectile function. Given that ageing results in a decline in erectile function, we determined changes in the expression of MaxiK, which might impact erectile function. Quantitative-polymerase chain reaction demonstrated that although there is no significant change in transcription of the α- and β-subunits that comprise the MaxiK channel, there are significant changes in the expression of transcripts encoding different splice variants. One transcript, SV1, is 13-fold increased in expression in the ageing rat corpora. SV1 has previously been reported to trap other isoforms of the MaxiK channel in the cytoplasm. Correlating with increased expression of SV1, we observed in older rats there is approximately a 13-fold decrease in MaxiK protein in the corpora cell membrane and a greater proportion is retained in the cytoplasm (approximately threefold). These experiments demonstrate that ageing of the corpora is accompanied by changes in alternative splicing and cellular localization of the MaxiK channel.
KW - Ageing
KW - Erectile dysfunction
KW - MaxiK channel
KW - Smooth muscle
KW - Splicing
UR - http://www.scopus.com/inward/record.url?scp=34347393334&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34347393334&partnerID=8YFLogxK
U2 - 10.1038/sj.ijir.3901541
DO - 10.1038/sj.ijir.3901541
M3 - Article
C2 - 17287835
AN - SCOPUS:34347393334
VL - 19
SP - 371
EP - 377
JO - International Journal of Impotence Research
JF - International Journal of Impotence Research
SN - 0955-9930
IS - 4
ER -