Ageing causes cytoplasmic retention of MaxiK channels in rat corporal smooth muscle cells

Kelvin Davies, Y. Stanevsky, Moses T. Tar, J. S. Chang, M. R. Chance, A. Melman

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The MaxiK channel plays a critical role in the regulation of corporal smooth muscle tone and thereby erectile function. Given that ageing results in a decline in erectile function, we determined changes in the expression of MaxiK, which might impact erectile function. Quantitative-polymerase chain reaction demonstrated that although there is no significant change in transcription of the α- and β-subunits that comprise the MaxiK channel, there are significant changes in the expression of transcripts encoding different splice variants. One transcript, SV1, is 13-fold increased in expression in the ageing rat corpora. SV1 has previously been reported to trap other isoforms of the MaxiK channel in the cytoplasm. Correlating with increased expression of SV1, we observed in older rats there is approximately a 13-fold decrease in MaxiK protein in the corpora cell membrane and a greater proportion is retained in the cytoplasm (approximately threefold). These experiments demonstrate that ageing of the corpora is accompanied by changes in alternative splicing and cellular localization of the MaxiK channel.

Original languageEnglish (US)
Pages (from-to)371-377
Number of pages7
JournalInternational Journal of Impotence Research
Volume19
Issue number4
DOIs
StatePublished - Jul 2007

Fingerprint

Large-Conductance Calcium-Activated Potassium Channels
Smooth Muscle Myocytes
Cytoplasm
Alternative Splicing
Smooth Muscle
Protein Isoforms
Cell Membrane
Polymerase Chain Reaction
Proteins

Keywords

  • Ageing
  • Erectile dysfunction
  • MaxiK channel
  • Smooth muscle
  • Splicing

ASJC Scopus subject areas

  • Urology

Cite this

Ageing causes cytoplasmic retention of MaxiK channels in rat corporal smooth muscle cells. / Davies, Kelvin; Stanevsky, Y.; Tar, Moses T.; Chang, J. S.; Chance, M. R.; Melman, A.

In: International Journal of Impotence Research, Vol. 19, No. 4, 07.2007, p. 371-377.

Research output: Contribution to journalArticle

@article{a00f8ce317574721afabc592934b9101,
title = "Ageing causes cytoplasmic retention of MaxiK channels in rat corporal smooth muscle cells",
abstract = "The MaxiK channel plays a critical role in the regulation of corporal smooth muscle tone and thereby erectile function. Given that ageing results in a decline in erectile function, we determined changes in the expression of MaxiK, which might impact erectile function. Quantitative-polymerase chain reaction demonstrated that although there is no significant change in transcription of the α- and β-subunits that comprise the MaxiK channel, there are significant changes in the expression of transcripts encoding different splice variants. One transcript, SV1, is 13-fold increased in expression in the ageing rat corpora. SV1 has previously been reported to trap other isoforms of the MaxiK channel in the cytoplasm. Correlating with increased expression of SV1, we observed in older rats there is approximately a 13-fold decrease in MaxiK protein in the corpora cell membrane and a greater proportion is retained in the cytoplasm (approximately threefold). These experiments demonstrate that ageing of the corpora is accompanied by changes in alternative splicing and cellular localization of the MaxiK channel.",
keywords = "Ageing, Erectile dysfunction, MaxiK channel, Smooth muscle, Splicing",
author = "Kelvin Davies and Y. Stanevsky and Tar, {Moses T.} and Chang, {J. S.} and Chance, {M. R.} and A. Melman",
year = "2007",
month = "7",
doi = "10.1038/sj.ijir.3901541",
language = "English (US)",
volume = "19",
pages = "371--377",
journal = "International Journal of Impotence Research",
issn = "0955-9930",
publisher = "Nature Publishing Group",
number = "4",

}

TY - JOUR

T1 - Ageing causes cytoplasmic retention of MaxiK channels in rat corporal smooth muscle cells

AU - Davies, Kelvin

AU - Stanevsky, Y.

AU - Tar, Moses T.

AU - Chang, J. S.

AU - Chance, M. R.

AU - Melman, A.

PY - 2007/7

Y1 - 2007/7

N2 - The MaxiK channel plays a critical role in the regulation of corporal smooth muscle tone and thereby erectile function. Given that ageing results in a decline in erectile function, we determined changes in the expression of MaxiK, which might impact erectile function. Quantitative-polymerase chain reaction demonstrated that although there is no significant change in transcription of the α- and β-subunits that comprise the MaxiK channel, there are significant changes in the expression of transcripts encoding different splice variants. One transcript, SV1, is 13-fold increased in expression in the ageing rat corpora. SV1 has previously been reported to trap other isoforms of the MaxiK channel in the cytoplasm. Correlating with increased expression of SV1, we observed in older rats there is approximately a 13-fold decrease in MaxiK protein in the corpora cell membrane and a greater proportion is retained in the cytoplasm (approximately threefold). These experiments demonstrate that ageing of the corpora is accompanied by changes in alternative splicing and cellular localization of the MaxiK channel.

AB - The MaxiK channel plays a critical role in the regulation of corporal smooth muscle tone and thereby erectile function. Given that ageing results in a decline in erectile function, we determined changes in the expression of MaxiK, which might impact erectile function. Quantitative-polymerase chain reaction demonstrated that although there is no significant change in transcription of the α- and β-subunits that comprise the MaxiK channel, there are significant changes in the expression of transcripts encoding different splice variants. One transcript, SV1, is 13-fold increased in expression in the ageing rat corpora. SV1 has previously been reported to trap other isoforms of the MaxiK channel in the cytoplasm. Correlating with increased expression of SV1, we observed in older rats there is approximately a 13-fold decrease in MaxiK protein in the corpora cell membrane and a greater proportion is retained in the cytoplasm (approximately threefold). These experiments demonstrate that ageing of the corpora is accompanied by changes in alternative splicing and cellular localization of the MaxiK channel.

KW - Ageing

KW - Erectile dysfunction

KW - MaxiK channel

KW - Smooth muscle

KW - Splicing

UR - http://www.scopus.com/inward/record.url?scp=34347393334&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34347393334&partnerID=8YFLogxK

U2 - 10.1038/sj.ijir.3901541

DO - 10.1038/sj.ijir.3901541

M3 - Article

C2 - 17287835

AN - SCOPUS:34347393334

VL - 19

SP - 371

EP - 377

JO - International Journal of Impotence Research

JF - International Journal of Impotence Research

SN - 0955-9930

IS - 4

ER -