Age-dependent loss of insulin-like growth factor-1 receptor immunoreactive cells in the supraoptic hypothalamus is reduced in calorically restricted mice

F. Yaghmaie, Omar Saeed, S. A. Garan, M. A. Voelker, A. M. Gouw, W. Freitag, H. Sternberg, P. S. Timiras

Research output: Contribution to journalArticle

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Abstract

Both life-long caloric restriction (CR) and the suppression of insulin-like growth factor-1 (IGF-1) signaling reliably extend the mammalian lifespan. The neuroendocrine system, regulated by the hypothalamus, remains the most convincing site of action for both these modes of life extension. Yet, determining whether CR actions are mediated by the modulation of neuroendocrine IGF-1 signaling remains unclear. Of the hypothalamic nuclei that express the IGF-1 receptor (IGF-1R), the cells of the supraoptic nucleus (SON) display some of the most robust IGF-1R expression. Taking IGF-1R immunoreactivity as an index of sensitivity to IGF-1, we counted IGF-1R immunoreactive and non-immunoreactive cells in the SON of young-ad-libitum fed (young-Al, 6 weeks), old-ad-libitum fed (Old-Al, 22 months), and old-calorie-restricted (Old-CR, 22 months) female B6D2F1 mice. An automated imaging microscopy system (AIMS) was used to generate cell counts for each section of supraoptic hypothalamus. Results show that while the total number of cells in the SON of ad-libitum fed mice does not change significantly with aging, a significant reduction in IGF-1R immunoreactive cells does occur in ad-libitum fed mice with aging. In contrast to this, calorie restricted mice show both a decline in the total number of cells and IGF-1R immunoreactive cells in the SON with age, but with the decrease in the latter being notably attenuated when compared to the degree of loss seen in ad-libitum fed mice. Thus, while CR induces greater loss in the total number of cells in the SON with age, it reduces the degree of age-dependent loss seen in IGF-1R expressing cells. As a result, when compared to Old-AL mice, the SON of Old-CR mice displays a greater proportion of IGF-1R cells and thus possibly enhanced IGF-1 sensitivity with aging.

Original languageEnglish (US)
Pages (from-to)431-436
Number of pages6
JournalInternational Journal of Developmental Neuroscience
Volume24
Issue number7
DOIs
StatePublished - Nov 2006
Externally publishedYes

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Somatomedin Receptors
Anterior Hypothalamus
IGF Type 1 Receptor
Supraoptic Nucleus
Caloric Restriction
Somatomedins
Cell Count
Neurosecretory Systems
Life Expectancy
Cell Nucleus
Hypothalamus
Microscopy

Keywords

  • Caloric restriction
  • Hypothalamus
  • IGF-1
  • IGF-1 receptor
  • IGF-1R
  • Six-week-old young mice
  • Supraoptic nucleus
  • Twenty-two-month-old mice

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

Cite this

Age-dependent loss of insulin-like growth factor-1 receptor immunoreactive cells in the supraoptic hypothalamus is reduced in calorically restricted mice. / Yaghmaie, F.; Saeed, Omar; Garan, S. A.; Voelker, M. A.; Gouw, A. M.; Freitag, W.; Sternberg, H.; Timiras, P. S.

In: International Journal of Developmental Neuroscience, Vol. 24, No. 7, 11.2006, p. 431-436.

Research output: Contribution to journalArticle

Yaghmaie, F. ; Saeed, Omar ; Garan, S. A. ; Voelker, M. A. ; Gouw, A. M. ; Freitag, W. ; Sternberg, H. ; Timiras, P. S. / Age-dependent loss of insulin-like growth factor-1 receptor immunoreactive cells in the supraoptic hypothalamus is reduced in calorically restricted mice. In: International Journal of Developmental Neuroscience. 2006 ; Vol. 24, No. 7. pp. 431-436.
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AU - Saeed, Omar

AU - Garan, S. A.

AU - Voelker, M. A.

AU - Gouw, A. M.

AU - Freitag, W.

AU - Sternberg, H.

AU - Timiras, P. S.

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