Age- and temperature-dependent somatic mutation accumulation in Drosophila melanogaster.

Ana Maria Garcia, R. Brent Calder, Martijn E.T. Dollé, Martha Lundell, Pankaj Kapahi, Jan Vijg

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Using a transgenic mouse model harboring a mutation reporter gene that can be efficiently recovered from genomic DNA, we previously demonstrated that mutations accumulate in aging mice in a tissue-specific manner. Applying a recently developed, similar reporter-based assay in Drosophila melanogaster, we now show that the mutation frequency at the lacZ locus in somatic tissue of flies is about three times as high as in mouse tissues, with a much higher fraction of large genome rearrangements. Similar to mice, somatic mutations in the fly also accumulate as a function of age, but they do so much more quickly at higher temperature, a condition which in invertebrates is associated with decreased life span. Most mutations were found to accumulate in the thorax and less in abdomen, suggesting the highly oxidative flight muscles as a possible source of genotoxic stress. These results show that somatic mutation loads in short-lived flies are much more severe than in the much longer-lived mice, with the mutation rate in flies proportional to biological rather than chronological aging.

Original languageEnglish (US)
JournalPLoS Genetics
Volume6
Issue number5
DOIs
StatePublished - 2010

Fingerprint

somatic mutation
Drosophila melanogaster
mutation
Diptera
Mutation
Temperature
mice
Mutation Rate
temperature
flight muscles
Invertebrates
thorax
Reporter Genes
reporter genes
abdomen
Abdomen
Transgenic Mice
DNA Damage
Thorax
animal models

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Ecology, Evolution, Behavior and Systematics
  • Cancer Research
  • Genetics(clinical)

Cite this

Age- and temperature-dependent somatic mutation accumulation in Drosophila melanogaster. / Garcia, Ana Maria; Calder, R. Brent; Dollé, Martijn E.T.; Lundell, Martha; Kapahi, Pankaj; Vijg, Jan.

In: PLoS Genetics, Vol. 6, No. 5, 2010.

Research output: Contribution to journalArticle

Garcia, Ana Maria ; Calder, R. Brent ; Dollé, Martijn E.T. ; Lundell, Martha ; Kapahi, Pankaj ; Vijg, Jan. / Age- and temperature-dependent somatic mutation accumulation in Drosophila melanogaster. In: PLoS Genetics. 2010 ; Vol. 6, No. 5.
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