Age- And stress-associated C. Elegans granulins impair lysosomal function and induce a compensatory HLH-30/TFEB transcriptional response

Victoria J. Butler, Fuying Gao, Christian I. Corrales, Wilian A. Cortopassi, Benjamin Caballero, Mihir Vohra, Kaveh Ashrafi, Ana Maria Cuervo, Matthew P. Jacobson, Giovanni Coppola, Aimee W. Kao

Research output: Contribution to journalArticle

Abstract

The progressive failure of protein homeostasis is a hallmark of aging and a common feature in neurodegenerative disease. As the enzymes executing the final stages of autophagy, lysosomal proteases are key contributors to the maintenance of protein homeostasis with age. We previously reported that expression of granulin peptides, the cleavage products of the neurodegenerative disease protein progranulin, enhance the accumulation and toxicity of TAR DNA binding protein 43 (TDP-43) in Caenorhabditis elegans (C. elegans). In this study we show that C. elegans granulins are produced in an age- and stress-dependent manner. Granulins localize to the endolysosomal compartment where they impair lysosomal protease expression and activity. Consequently, protein homeostasis is disrupted, promoting the nuclear translocation of the lysosomal transcription factor HLH-30/TFEB, and prompting cells to activate a compensatory transcriptional program. The three C. elegans granulin peptides exhibited distinct but overlapping functional effects in our assays, which may be due to amino acid composition that results in distinct electrostatic and hydrophobicity profiles. Our results support a model in which granulin production modulates a critical transition between the normal, physiological regulation of protease activity and the impairment of lysosomal function that can occur with age and disease.

Original languageEnglish (US)
Article numbere1008295
JournalPLoS genetics
Volume15
Issue number8
DOIs
StatePublished - Jan 1 2019

Fingerprint

Caenorhabditis elegans
homeostasis
protein
proteinases
neurodegenerative diseases
Homeostasis
Peptide Hydrolases
proteins
Neurodegenerative Diseases
peptide
peptides
physiological regulation
Proteins
DNA-binding proteins
autophagy
hydrophobicity
Peptides
amino acid composition
Autophagy
DNA-Binding Proteins

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Genetics(clinical)
  • Cancer Research

Cite this

Butler, V. J., Gao, F., Corrales, C. I., Cortopassi, W. A., Caballero, B., Vohra, M., ... Kao, A. W. (2019). Age- And stress-associated C. Elegans granulins impair lysosomal function and induce a compensatory HLH-30/TFEB transcriptional response. PLoS genetics, 15(8), [e1008295]. https://doi.org/10.1371/journal.pgen.1008295

Age- And stress-associated C. Elegans granulins impair lysosomal function and induce a compensatory HLH-30/TFEB transcriptional response. / Butler, Victoria J.; Gao, Fuying; Corrales, Christian I.; Cortopassi, Wilian A.; Caballero, Benjamin; Vohra, Mihir; Ashrafi, Kaveh; Cuervo, Ana Maria; Jacobson, Matthew P.; Coppola, Giovanni; Kao, Aimee W.

In: PLoS genetics, Vol. 15, No. 8, e1008295, 01.01.2019.

Research output: Contribution to journalArticle

Butler, VJ, Gao, F, Corrales, CI, Cortopassi, WA, Caballero, B, Vohra, M, Ashrafi, K, Cuervo, AM, Jacobson, MP, Coppola, G & Kao, AW 2019, 'Age- And stress-associated C. Elegans granulins impair lysosomal function and induce a compensatory HLH-30/TFEB transcriptional response', PLoS genetics, vol. 15, no. 8, e1008295. https://doi.org/10.1371/journal.pgen.1008295
Butler, Victoria J. ; Gao, Fuying ; Corrales, Christian I. ; Cortopassi, Wilian A. ; Caballero, Benjamin ; Vohra, Mihir ; Ashrafi, Kaveh ; Cuervo, Ana Maria ; Jacobson, Matthew P. ; Coppola, Giovanni ; Kao, Aimee W. / Age- And stress-associated C. Elegans granulins impair lysosomal function and induce a compensatory HLH-30/TFEB transcriptional response. In: PLoS genetics. 2019 ; Vol. 15, No. 8.
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