Advances in lectin microarray technology: optimized protocols for piezoelectric print conditions

Kanoelani T. Pilobello, Praveen Agrawal, Richard Rouse, Lara K. Mahal

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Lectin microarray technology has been used to profile the glycosylation of a multitude of biological and clinical samples, leading to new clinical biomarkers and advances in glycobiology. Lectin microarrays, which include >90 plant lectins, recombinant lectins, and selected antibodies, are used to profile N-linked, O-linked, and glycolipid glycans. The specificity and depth of glycan profiling depends upon the carbohydrate-binding proteins arrayed. The current set targets mammalian carbohydrates including fucose, high mannose, branched and complex N-linked, α- and β-galactose and GalNAc, α-2,3- and α-2,6-sialic acid, LacNAc, and Lewis X epitopes. Previous protocols have described the use of a contact microarray printer for lectin microarray production. Here, an updated protocol that uses a non-contact, piezoelectric printer, which leads to increased lectin activity on the array, is presented. Optimization of print and sample hybridization conditions and methods of analysis are discussed.

Original languageEnglish (US)
Pages (from-to)1-23
Number of pages23
JournalCurrent protocols in chemical biology
Volume5
Issue number1
DOIs
StatePublished - 2013
Externally publishedYes

Keywords

  • carbohydrate analysis
  • glycomics
  • lectin microarray
  • Nano‐Plotter
  • piezoelectric

ASJC Scopus subject areas

  • Medicine(all)

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