Adipocyte-derived collagen VI affects early mammary tumor progression in vivo, demonstrating a critical interaction in the tumor/stroma microenvironment

Puneeth Iyengar, Virginia Espina, Terence W. Williams, Ying Lin, David Berry, Linda A. Jelicks, Hyangkyu Lee, Karla Temple, Reed Graves, Jeffrey Pollard, Neeru Chopra, Robert G. Russell, Ram Sasisekharan, Bruce J. Trock, Marc Lippman, Valerie S. Calvert, Emanuel F. Petricoin, Lance Liotta, Ekaterina Dadachova, Richard G. Pestell & 3 others Michael P. Lisanti, Paolo Bonaldo, Philipp E. Scherer

Research output: Contribution to journalArticle

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Abstract

The interactions of transformed cells with the surrounding stromal cells are of importance for tumor progression and metastasis. The relevance of adipocyte-derived factors to breast cancer cell survival and growth is well established. However, it remains unknown which specific adipocyte-derived factors are most critical in this process. Collagen VI is abundantly expressed in adipocytes. Collagen-/- mice in the background of the mouse mammary tumor virus/polyoma virus middle T oncogene (MMTV-PyMT) mammary cancer model demonstrate dramatically reduced rates of early hyperplasia and primary tumor growth. Collagen VI promotes its growth-stimulatory and pro-survival effects in part by signaling through the NG2/chondroitin sulfate proteoglycan receptor expressed on the surface of malignant ductal epithelial cells to sequentially activate Akt and β-catenin and stabilize cyclin D1. Levels of the carboxyterminal domain of collagen VIα3, a proteolytic product of the full-length molecule, are dramatically upregulated in murine and human breast cancer lesions. The same fragment exerts potent growth-stimulatory effects on MCF-7 cells in vitro. Therefore, adipocytes play a vital role in defining the ECM environment for normal and tumor-derived ductal epithelial cells and contribute significantly to tumor growth at early stages through secretion and processing of collagen VI.

Original languageEnglish (US)
Pages (from-to)1163-1176
Number of pages14
JournalJournal of Clinical Investigation
Volume115
Issue number5
DOIs
StatePublished - May 2005

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Tumor Microenvironment
Adipocytes
Collagen
Breast Neoplasms
Growth
Neoplasms
Epithelial Cells
Chondroitin Sulfate Proteoglycans
Mouse mammary tumor virus
Catenins
Polyomavirus
Cyclin D1
MCF-7 Cells
Stromal Cells
Oncogenes
Cell Communication
Hyperplasia
Cell Survival
Neoplasm Metastasis
Survival

ASJC Scopus subject areas

  • Medicine(all)

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Adipocyte-derived collagen VI affects early mammary tumor progression in vivo, demonstrating a critical interaction in the tumor/stroma microenvironment. / Iyengar, Puneeth; Espina, Virginia; Williams, Terence W.; Lin, Ying; Berry, David; Jelicks, Linda A.; Lee, Hyangkyu; Temple, Karla; Graves, Reed; Pollard, Jeffrey; Chopra, Neeru; Russell, Robert G.; Sasisekharan, Ram; Trock, Bruce J.; Lippman, Marc; Calvert, Valerie S.; Petricoin, Emanuel F.; Liotta, Lance; Dadachova, Ekaterina; Pestell, Richard G.; Lisanti, Michael P.; Bonaldo, Paolo; Scherer, Philipp E.

In: Journal of Clinical Investigation, Vol. 115, No. 5, 05.2005, p. 1163-1176.

Research output: Contribution to journalArticle

Iyengar, P, Espina, V, Williams, TW, Lin, Y, Berry, D, Jelicks, LA, Lee, H, Temple, K, Graves, R, Pollard, J, Chopra, N, Russell, RG, Sasisekharan, R, Trock, BJ, Lippman, M, Calvert, VS, Petricoin, EF, Liotta, L, Dadachova, E, Pestell, RG, Lisanti, MP, Bonaldo, P & Scherer, PE 2005, 'Adipocyte-derived collagen VI affects early mammary tumor progression in vivo, demonstrating a critical interaction in the tumor/stroma microenvironment', Journal of Clinical Investigation, vol. 115, no. 5, pp. 1163-1176. https://doi.org/10.1172/JCI200523424
Iyengar, Puneeth ; Espina, Virginia ; Williams, Terence W. ; Lin, Ying ; Berry, David ; Jelicks, Linda A. ; Lee, Hyangkyu ; Temple, Karla ; Graves, Reed ; Pollard, Jeffrey ; Chopra, Neeru ; Russell, Robert G. ; Sasisekharan, Ram ; Trock, Bruce J. ; Lippman, Marc ; Calvert, Valerie S. ; Petricoin, Emanuel F. ; Liotta, Lance ; Dadachova, Ekaterina ; Pestell, Richard G. ; Lisanti, Michael P. ; Bonaldo, Paolo ; Scherer, Philipp E. / Adipocyte-derived collagen VI affects early mammary tumor progression in vivo, demonstrating a critical interaction in the tumor/stroma microenvironment. In: Journal of Clinical Investigation. 2005 ; Vol. 115, No. 5. pp. 1163-1176.
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AU - Berry, David

AU - Jelicks, Linda A.

AU - Lee, Hyangkyu

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AU - Graves, Reed

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AU - Russell, Robert G.

AU - Sasisekharan, Ram

AU - Trock, Bruce J.

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AU - Calvert, Valerie S.

AU - Petricoin, Emanuel F.

AU - Liotta, Lance

AU - Dadachova, Ekaterina

AU - Pestell, Richard G.

AU - Lisanti, Michael P.

AU - Bonaldo, Paolo

AU - Scherer, Philipp E.

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N2 - The interactions of transformed cells with the surrounding stromal cells are of importance for tumor progression and metastasis. The relevance of adipocyte-derived factors to breast cancer cell survival and growth is well established. However, it remains unknown which specific adipocyte-derived factors are most critical in this process. Collagen VI is abundantly expressed in adipocytes. Collagen-/- mice in the background of the mouse mammary tumor virus/polyoma virus middle T oncogene (MMTV-PyMT) mammary cancer model demonstrate dramatically reduced rates of early hyperplasia and primary tumor growth. Collagen VI promotes its growth-stimulatory and pro-survival effects in part by signaling through the NG2/chondroitin sulfate proteoglycan receptor expressed on the surface of malignant ductal epithelial cells to sequentially activate Akt and β-catenin and stabilize cyclin D1. Levels of the carboxyterminal domain of collagen VIα3, a proteolytic product of the full-length molecule, are dramatically upregulated in murine and human breast cancer lesions. The same fragment exerts potent growth-stimulatory effects on MCF-7 cells in vitro. Therefore, adipocytes play a vital role in defining the ECM environment for normal and tumor-derived ductal epithelial cells and contribute significantly to tumor growth at early stages through secretion and processing of collagen VI.

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