Adenosine modulates methylmercuric chloride (MeHgCl)-inducedd-aspartate release from neonatal rat primary astrocyte cultures

M. Aschner, K. J. Mullaney, D. E. Wagoner, L. H. Lash, H. K. Kimelberg

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

The effects of adenosine, and selective adenosine receptor agonists and antagonists on methylmercury (MeHg)-induced aspartate release were studied in neonatal rat primary astrocyte cultures. Whereas basal levels ofd-[3H]aspartate release were unchanged upon treatment with adenosine or selective A1 receptor agonists, N6-cyclopentyladenosine (CPA), cyclohexyladenosine (CHA), and R-phenylisopropyladenosine (R-PIA), all partially reversed the MeHg-induced release ofd-aspartate. Treatment of astrocytes with the xanthine derivative, theophylline, an adenosine antagonist, reversed the inhibitory effect of adenosine on MeHg-inducedd-[3H]aspartate release. Since the effect of MeHg ond-[3H]aspartate release is known to be associated with sulfhydryl (-SH) groups which are controlled by intracellular glutathione concentrations [GSH]i, we also evaluated the effects of adenosine, the A1 agonists CPA and CHP, and the adenosine antagonist, theophylline, on astrocytic [GSH]i. Attenuation of the stimulatory effect of MeHg ond-[3H]aspartate release by adenosine and its agonists occurred in the presence of reduced astrocytic [GSH]i, suggesting that other mechanisms must be invoked for this protective effect. Whilst the mechanism of MeHg-inducedd-[3H]aspartate release is not known, the data suggest a role for adenosine in its regulation.

Original languageEnglish (US)
Pages (from-to)1-8
Number of pages8
JournalBrain research
Volume689
Issue number1
DOIs
StatePublished - Aug 14 1995
Externally publishedYes

Keywords

  • Aspartate
  • Astrocyte
  • Glutamate
  • Glutathione
  • Methylmercury
  • Rat

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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