Adenosine modulates methylmercuric chloride (MeHgCl)-inducedd-aspartate release from neonatal rat primary astrocyte cultures

Michael Aschner, K. J. Mullaney, D. E. Wagoner, L. H. Lash, H. K. Kimelberg

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The effects of adenosine, and selective adenosine receptor agonists and antagonists on methylmercury (MeHg)-induced aspartate release were studied in neonatal rat primary astrocyte cultures. Whereas basal levels ofd-[3H]aspartate release were unchanged upon treatment with adenosine or selective A1 receptor agonists, N6-cyclopentyladenosine (CPA), cyclohexyladenosine (CHA), and R-phenylisopropyladenosine (R-PIA), all partially reversed the MeHg-induced release ofd-aspartate. Treatment of astrocytes with the xanthine derivative, theophylline, an adenosine antagonist, reversed the inhibitory effect of adenosine on MeHg-inducedd-[3H]aspartate release. Since the effect of MeHg ond-[3H]aspartate release is known to be associated with sulfhydryl (-SH) groups which are controlled by intracellular glutathione concentrations [GSH]i, we also evaluated the effects of adenosine, the A1 agonists CPA and CHP, and the adenosine antagonist, theophylline, on astrocytic [GSH]i. Attenuation of the stimulatory effect of MeHg ond-[3H]aspartate release by adenosine and its agonists occurred in the presence of reduced astrocytic [GSH]i, suggesting that other mechanisms must be invoked for this protective effect. Whilst the mechanism of MeHg-inducedd-[3H]aspartate release is not known, the data suggest a role for adenosine in its regulation.

Original languageEnglish (US)
Pages (from-to)1-8
Number of pages8
JournalBrain Research
Volume689
Issue number1
DOIs
StatePublished - Aug 14 1995
Externally publishedYes

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Aspartic Acid
Astrocytes
Adenosine
Theophylline
Phenylisopropyladenosine
Purinergic P1 Receptor Agonists
Purinergic P1 Receptor Antagonists
Xanthine
methylmercuric chloride
Glutathione

Keywords

  • Aspartate
  • Astrocyte
  • Glutamate
  • Glutathione
  • Methylmercury
  • Rat

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Adenosine modulates methylmercuric chloride (MeHgCl)-inducedd-aspartate release from neonatal rat primary astrocyte cultures. / Aschner, Michael; Mullaney, K. J.; Wagoner, D. E.; Lash, L. H.; Kimelberg, H. K.

In: Brain Research, Vol. 689, No. 1, 14.08.1995, p. 1-8.

Research output: Contribution to journalArticle

Aschner, Michael ; Mullaney, K. J. ; Wagoner, D. E. ; Lash, L. H. ; Kimelberg, H. K. / Adenosine modulates methylmercuric chloride (MeHgCl)-inducedd-aspartate release from neonatal rat primary astrocyte cultures. In: Brain Research. 1995 ; Vol. 689, No. 1. pp. 1-8.
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