Acute Streptococcus pneumoniae meningogenic labyrinthitis: An experimental guinea pig model and literature review

A. L. Blank, G. L. Davis, T. R. VanDeWater, Robert J. Ruben

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Objective: To create an experimental model of Streptococcus pneumoniae type 3 meningogenic labyrinthitis (a leading cause of deafness) similar to that in human disease. Design: Cohort analytic study of guinea pigs that were inoculated intrathecally with varying dilutions of S pneumoniae type 3; the progress of the disease was compared with that in saline solution-inoculated control animals. Subjects: Healthy adult Hartley guinea pigs without clinical evidence of middle ear disease that were conveniently sampled. Interventions: Intrathecal inoculation of 104 to 106 colony-forming units of S pneumoniae type 3 into 13 guinea pigs; signs and symptoms of meningitis/labyrinthitis were observed for 15 days and compared with those in two saline solution- inoculated control animals. Main Outcome Measures: Morbidity-labyrinthitis, meningitis; end point-death. Results: The 104 to 106 colony-forming units of S pneumoniae type 3 caused inflammation that extended from the meninges to the inner ear via the cochlear aqueduct within 3 days after inoculation; a dose of 107 killed animals within 12 hours after inoculation. Three of five animals that were inoculated with a 106 dose died 3 days after inoculation; two of three animals that were inoculated with a 105 dose lived to 15 days after inoculation. One of two animals that were inoculated with a 104 dose did not become infected. Inflammation extended to the middle ear by round- window destruction. Reactive bone formation simulated labyrinthine osteosclerosis. Observers assessed histologic slides 'blindly.' Conclusion: Guinea pigs can survive 15 days after intrathecal inoculation of a 105 dose, with morphologic features similar to those in human disease. This is an effective model for this study of meningogenic labyrinthitis.

Original languageEnglish (US)
Pages (from-to)1342-1346
Number of pages5
JournalArchives of Otolaryngology - Head and Neck Surgery
Volume120
Issue number12
StatePublished - 1994

Fingerprint

Labyrinthitis
Streptococcus pneumoniae
Guinea Pigs
Pneumonia
Middle Ear
Meningitis
Sodium Chloride
Ear Round Window
Cochlear Aqueduct
Stem Cells
Osteosclerosis
Inflammation
Ear Diseases
Meninges
Deafness
Inner Ear
Osteogenesis
Signs and Symptoms
Healthy Volunteers
Cohort Studies

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Acute Streptococcus pneumoniae meningogenic labyrinthitis : An experimental guinea pig model and literature review. / Blank, A. L.; Davis, G. L.; VanDeWater, T. R.; Ruben, Robert J.

In: Archives of Otolaryngology - Head and Neck Surgery, Vol. 120, No. 12, 1994, p. 1342-1346.

Research output: Contribution to journalArticle

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