Acute no-effect dose for in ova exposure to C3F7 tagged 5-hydroxytryptophan, a novel probe for investigating neural development

Sherry Dingman; Lara Hurlburt; Craig Branch

doi:10.1016/j.mibio.2003.11.001

Acute no-effect dose for in ova exposure to C₃F₇ tagged 5-hydroxytryptophan, a novel probe for investigating neural development

Sherry Dingman, Lara Hurlburt, Craig Branch

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Purpose: To create a probe for investigating neural development in chicks using magnetic resonance, a C₃F₇ tag was bonded to the indole ring of 5-hydroxytryptophan (5-HTP). Immunoassays indicate that a low dose (5 μg/egg) administered in ova results in 0.5-1.0 μg accumulating in a 250 μL midbrain. Procedures: Given the potential for developmental neurotoxicity of a compound that replaces an endogenous neurotransmitter, we investigated the effects of acute exposure during development. Results: No qualitative differences were observed between treatment groups. Differences in mortality rates between treatment groups were not statistically significant [ X²_obs = 2.30, X² _crit (df=2)=5.99, P > 05]. Conclusion: The no-effect dose for in ova administration of C₃F₇ tagged5-HTP is 5 μg/egg on Day 17.

Original language	English (US)
Pages (from-to)	12-16
Number of pages	5
Journal	Molecular Imaging and Biology
Volume	6
Issue number	1
DOIs	https://doi.org/10.1016/j.mibio.2003.11.001
State	Published - Jan 2004
Externally published	Yes

Keywords

5-hydroxytryptophan
Developmental neurotoxicity
F
In ova chicks
Magnetic resonance
Monoamines
Neurotransmitters
No-effect dose

ASJC Scopus subject areas

Oncology
Radiology Nuclear Medicine and imaging
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.mibio.2003.11.001

Cite this

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title = "Acute no-effect dose for in ova exposure to C3F7 tagged 5-hydroxytryptophan, a novel probe for investigating neural development",

abstract = "Purpose: To create a probe for investigating neural development in chicks using magnetic resonance, a C3F7 tag was bonded to the indole ring of 5-hydroxytryptophan (5-HTP). Immunoassays indicate that a low dose (5 μg/egg) administered in ova results in 0.5-1.0 μg accumulating in a 250 μL midbrain. Procedures: Given the potential for developmental neurotoxicity of a compound that replaces an endogenous neurotransmitter, we investigated the effects of acute exposure during development. Results: No qualitative differences were observed between treatment groups. Differences in mortality rates between treatment groups were not statistically significant [ X2obs = 2.30, X2 crit (df=2)=5.99, P > 05]. Conclusion: The no-effect dose for in ova administration of C3F7 tagged5-HTP is 5 μg/egg on Day 17.",

keywords = "5-hydroxytryptophan, Developmental neurotoxicity, F, In ova chicks, Magnetic resonance, Monoamines, Neurotransmitters, No-effect dose",

author = "Sherry Dingman and Lara Hurlburt and Craig Branch",

year = "2004",

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doi = "10.1016/j.mibio.2003.11.001",

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journal = "Molecular Imaging and Biology",

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AU - Hurlburt, Lara

AU - Branch, Craig

PY - 2004/1

Y1 - 2004/1

N2 - Purpose: To create a probe for investigating neural development in chicks using magnetic resonance, a C3F7 tag was bonded to the indole ring of 5-hydroxytryptophan (5-HTP). Immunoassays indicate that a low dose (5 μg/egg) administered in ova results in 0.5-1.0 μg accumulating in a 250 μL midbrain. Procedures: Given the potential for developmental neurotoxicity of a compound that replaces an endogenous neurotransmitter, we investigated the effects of acute exposure during development. Results: No qualitative differences were observed between treatment groups. Differences in mortality rates between treatment groups were not statistically significant [ X2obs = 2.30, X2 crit (df=2)=5.99, P > 05]. Conclusion: The no-effect dose for in ova administration of C3F7 tagged5-HTP is 5 μg/egg on Day 17.

AB - Purpose: To create a probe for investigating neural development in chicks using magnetic resonance, a C3F7 tag was bonded to the indole ring of 5-hydroxytryptophan (5-HTP). Immunoassays indicate that a low dose (5 μg/egg) administered in ova results in 0.5-1.0 μg accumulating in a 250 μL midbrain. Procedures: Given the potential for developmental neurotoxicity of a compound that replaces an endogenous neurotransmitter, we investigated the effects of acute exposure during development. Results: No qualitative differences were observed between treatment groups. Differences in mortality rates between treatment groups were not statistically significant [ X2obs = 2.30, X2 crit (df=2)=5.99, P > 05]. Conclusion: The no-effect dose for in ova administration of C3F7 tagged5-HTP is 5 μg/egg on Day 17.

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KW - Monoamines

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KW - No-effect dose

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