Acute no-effect dose for in ova exposure to C3F7 tagged 5-hydroxytryptophan, a novel probe for investigating neural development

Sherry Dingman, Lara Hurlburt, Craig Branch

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Purpose: To create a probe for investigating neural development in chicks using magnetic resonance, a C3F7 tag was bonded to the indole ring of 5-hydroxytryptophan (5-HTP). Immunoassays indicate that a low dose (5 μg/egg) administered in ova results in 0.5-1.0 μg accumulating in a 250 μL midbrain. Procedures: Given the potential for developmental neurotoxicity of a compound that replaces an endogenous neurotransmitter, we investigated the effects of acute exposure during development. Results: No qualitative differences were observed between treatment groups. Differences in mortality rates between treatment groups were not statistically significant [ X2obs = 2.30, X2 crit (df=2)=5.99, P > 05]. Conclusion: The no-effect dose for in ova administration of C3F7 tagged5-HTP is 5 μg/egg on Day 17.

Original languageEnglish (US)
Pages (from-to)12-16
Number of pages5
JournalMolecular Imaging and Biology
Volume6
Issue number1
DOIs
StatePublished - Jan 1 2004
Externally publishedYes

Keywords

  • 5-hydroxytryptophan
  • Developmental neurotoxicity
  • F
  • In ova chicks
  • Magnetic resonance
  • Monoamines
  • Neurotransmitters
  • No-effect dose

ASJC Scopus subject areas

  • Oncology
  • Radiology Nuclear Medicine and imaging
  • Cancer Research

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