TY - JOUR
T1 - Acute glufosinate-based herbicide treatment in rats leads to increased ocular interleukin-1β and c-Fos protein levels, as well as intraocular pressure
AU - Çomaklı, Selim
AU - Sevim, Çiğdem
AU - Kontadakis, George
AU - Doğan, Elif
AU - Taghizadehghalehjoughi, Ali
AU - Özkaraca, Mustafa
AU - Aschner, Michael
AU - Nikolouzakis, Taxiarchis Konstantinos
AU - Tsatsakis, Aristides
N1 - Publisher Copyright:
© 2019 The Authors
PY - 2019
Y1 - 2019
N2 - Glufosinate is a common herbicide with neurotoxic effects, leading to seizures, convulsions and memory loss. Glufosinate indirectly induces glutamate toxicity by inhibiting glutamine synthesis in astrocytes. Here, we studied the acute toxic effects of a glufosinate-based herbicide in rat optic nerve at three doses (40, 80 or 120 μM, equal to 714 or 21 mg/kg bw/day). Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), urea, glucose, calcium, as well as creatinine concentrations were analyzed after 24, 48 and 72 h treatment. Intraocular pressure (IOP) (expressed as the average of both eyes) was measured with a rebound tonometer. Interleukin-1β (IL-1β) and c-Fos expression were determined by immunohistochemistry. The results established that the glufosinate-based herbicide significantly increased IL-1β and c-Fos immunopositivity in the optic nerve (p < 0.05), concomitant with increased IOP. These results suggest that commercial formulations of glufosinate acutely affect the optic nerve.
AB - Glufosinate is a common herbicide with neurotoxic effects, leading to seizures, convulsions and memory loss. Glufosinate indirectly induces glutamate toxicity by inhibiting glutamine synthesis in astrocytes. Here, we studied the acute toxic effects of a glufosinate-based herbicide in rat optic nerve at three doses (40, 80 or 120 μM, equal to 714 or 21 mg/kg bw/day). Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), urea, glucose, calcium, as well as creatinine concentrations were analyzed after 24, 48 and 72 h treatment. Intraocular pressure (IOP) (expressed as the average of both eyes) was measured with a rebound tonometer. Interleukin-1β (IL-1β) and c-Fos expression were determined by immunohistochemistry. The results established that the glufosinate-based herbicide significantly increased IL-1β and c-Fos immunopositivity in the optic nerve (p < 0.05), concomitant with increased IOP. These results suggest that commercial formulations of glufosinate acutely affect the optic nerve.
KW - Glufosinate
KW - Interleukin-1β
KW - Intraocular pressure
KW - c-fos
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U2 - 10.1016/j.toxrep.2019.01.004
DO - 10.1016/j.toxrep.2019.01.004
M3 - Article
AN - SCOPUS:85060293137
SN - 2214-7500
VL - 6
SP - 155
EP - 160
JO - Toxicology Reports
JF - Toxicology Reports
ER -