Abstract
In the course of our search for anti-dormant mycobacterial substances from marine organisms, we previously isolated three new aminolipopeptides, named trichoderins A, A1 and B, from the culture of the marine sponge-derived fungus of Trichoderma sp. and determined their chemical structures. To identify the gene that could confer a resistance to trichoderin A, we prepared transformants of Mycobacterium (M.) smegmatis, which were transformed with the genomic DNA library of M. bovis BCG constructed in the multi-copy shuttle cosmid pYUB145. Then, the transformant of M. smegmatis, which over-expressed a part of genes that coded mycobacterial ATP synthase, was found to exhibit a resistance to trichoderin A. In addition, trichoderin A reduced ATP contents in M. bovis BCG. These findings elucidated that the anti-mycobacterial activity of trichoderins comes from the inhibition of ATP synthesis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1287-1290 |
| Number of pages | 4 |
| Journal | Biological and Pharmaceutical Bulletin |
| Volume | 34 |
| Issue number | 8 |
| DOIs | |
| State | Published - Aug 1 2011 |
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Keywords
- Aminolipopeptide
- Anti-mycobacterial activity
- ATP synthase
- Dormant
- Trichoderin A
- Tuberculosis
ASJC Scopus subject areas
- Pharmacology
- Pharmaceutical Science
Cite this
Action-mechanism of trichoderin A, an anti-dormant mycobacterial aminolipopeptide from marine sponge-derived Trichoderma sp. / Pruksakorn, Patamaporn; Arai, Masayoshi; Liu, Liu; Moodley, Prashini; Jacobs, William Robert; Kobayashi, Motomasa.
In: Biological and Pharmaceutical Bulletin, Vol. 34, No. 8, 01.08.2011, p. 1287-1290.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Action-mechanism of trichoderin A, an anti-dormant mycobacterial aminolipopeptide from marine sponge-derived Trichoderma sp.
AU - Pruksakorn, Patamaporn
AU - Arai, Masayoshi
AU - Liu, Liu
AU - Moodley, Prashini
AU - Jacobs, William Robert
AU - Kobayashi, Motomasa
PY - 2011/8/1
Y1 - 2011/8/1
N2 - In the course of our search for anti-dormant mycobacterial substances from marine organisms, we previously isolated three new aminolipopeptides, named trichoderins A, A1 and B, from the culture of the marine sponge-derived fungus of Trichoderma sp. and determined their chemical structures. To identify the gene that could confer a resistance to trichoderin A, we prepared transformants of Mycobacterium (M.) smegmatis, which were transformed with the genomic DNA library of M. bovis BCG constructed in the multi-copy shuttle cosmid pYUB145. Then, the transformant of M. smegmatis, which over-expressed a part of genes that coded mycobacterial ATP synthase, was found to exhibit a resistance to trichoderin A. In addition, trichoderin A reduced ATP contents in M. bovis BCG. These findings elucidated that the anti-mycobacterial activity of trichoderins comes from the inhibition of ATP synthesis.
AB - In the course of our search for anti-dormant mycobacterial substances from marine organisms, we previously isolated three new aminolipopeptides, named trichoderins A, A1 and B, from the culture of the marine sponge-derived fungus of Trichoderma sp. and determined their chemical structures. To identify the gene that could confer a resistance to trichoderin A, we prepared transformants of Mycobacterium (M.) smegmatis, which were transformed with the genomic DNA library of M. bovis BCG constructed in the multi-copy shuttle cosmid pYUB145. Then, the transformant of M. smegmatis, which over-expressed a part of genes that coded mycobacterial ATP synthase, was found to exhibit a resistance to trichoderin A. In addition, trichoderin A reduced ATP contents in M. bovis BCG. These findings elucidated that the anti-mycobacterial activity of trichoderins comes from the inhibition of ATP synthesis.
KW - Aminolipopeptide
KW - Anti-mycobacterial activity
KW - ATP synthase
KW - Dormant
KW - Trichoderin A
KW - Tuberculosis
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UR - http://www.scopus.com/inward/citedby.url?scp=80051510564&partnerID=8YFLogxK
U2 - 10.1248/bpb.34.1287
DO - 10.1248/bpb.34.1287
M3 - Article
C2 - 21804219
AN - SCOPUS:80051510564
VL - 34
SP - 1287
EP - 1290
JO - Biological and Pharmaceutical Bulletin
JF - Biological and Pharmaceutical Bulletin
SN - 0918-6158
IS - 8
ER -