Action-mechanism of trichoderin A, an anti-dormant mycobacterial aminolipopeptide from marine sponge-derived Trichoderma sp.

Patamaporn Pruksakorn, Masayoshi Arai, Liu Liu, Prashini Moodley, William Robert Jacobs, Motomasa Kobayashi

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

In the course of our search for anti-dormant mycobacterial substances from marine organisms, we previously isolated three new aminolipopeptides, named trichoderins A, A1 and B, from the culture of the marine sponge-derived fungus of Trichoderma sp. and determined their chemical structures. To identify the gene that could confer a resistance to trichoderin A, we prepared transformants of Mycobacterium (M.) smegmatis, which were transformed with the genomic DNA library of M. bovis BCG constructed in the multi-copy shuttle cosmid pYUB145. Then, the transformant of M. smegmatis, which over-expressed a part of genes that coded mycobacterial ATP synthase, was found to exhibit a resistance to trichoderin A. In addition, trichoderin A reduced ATP contents in M. bovis BCG. These findings elucidated that the anti-mycobacterial activity of trichoderins comes from the inhibition of ATP synthesis.

Original languageEnglish (US)
Pages (from-to)1287-1290
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume34
Issue number8
DOIs
StatePublished - Aug 1 2011

Fingerprint

Trichoderma
Porifera
Adenosine Triphosphate
Mycobacterium bovis
Smegma
Mycobacterium smegmatis
Aquatic Organisms
Cosmids
Genomic Library
Gene Library
Genes
Fungi
trichoderin A

Keywords

  • Aminolipopeptide
  • Anti-mycobacterial activity
  • ATP synthase
  • Dormant
  • Trichoderin A
  • Tuberculosis

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

Cite this

Action-mechanism of trichoderin A, an anti-dormant mycobacterial aminolipopeptide from marine sponge-derived Trichoderma sp. / Pruksakorn, Patamaporn; Arai, Masayoshi; Liu, Liu; Moodley, Prashini; Jacobs, William Robert; Kobayashi, Motomasa.

In: Biological and Pharmaceutical Bulletin, Vol. 34, No. 8, 01.08.2011, p. 1287-1290.

Research output: Contribution to journalArticle

Pruksakorn, Patamaporn ; Arai, Masayoshi ; Liu, Liu ; Moodley, Prashini ; Jacobs, William Robert ; Kobayashi, Motomasa. / Action-mechanism of trichoderin A, an anti-dormant mycobacterial aminolipopeptide from marine sponge-derived Trichoderma sp. In: Biological and Pharmaceutical Bulletin. 2011 ; Vol. 34, No. 8. pp. 1287-1290.
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