Actin participation in actomyosin contraction

Teru Hayashi, Raja Rosenbluth, Peter Satir, Michael Vozick

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

1. 1. Pellicular fibers made of actin-free myosin show no ability to contract, at either pH 7.6 or 9.0. 2. 2. Fibers of actomyosin contract strongly and optimally at pH 7.6, but do not contract at pH 9.0. 3. 3. With increasing actin content, up to approximately 27%, the actomyosin fibers exhibit an increasing contractile activity. 4. 4. Fibers formed of actomyosin and of myosin hydrolyze ATP enzymically, the former at 7.6, the latter at pH 6.4 and 9.5. 5. 5. Myosin fibers, formed and maintained in the cold, do not contract when the temperature is raised in the presence of ATP, whereas actomyosin fibers under the same conditions, contract strongly with the rise in temperature. 6. 6. It is concluded that myosin without actin does not contract, and that actin is a specific requirement for contraction in the muscle protein model system.

Original languageEnglish (US)
Pages (from-to)1-8
Number of pages8
JournalBBA - Biochimica et Biophysica Acta
Volume28
Issue numberC
StatePublished - 1958
Externally publishedYes

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Actomyosin
Myosins
Actins
Fibers
Adenosine Triphosphate
Temperature
Muscle Proteins

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Hayashi, T., Rosenbluth, R., Satir, P., & Vozick, M. (1958). Actin participation in actomyosin contraction. BBA - Biochimica et Biophysica Acta, 28(C), 1-8.

Actin participation in actomyosin contraction. / Hayashi, Teru; Rosenbluth, Raja; Satir, Peter; Vozick, Michael.

In: BBA - Biochimica et Biophysica Acta, Vol. 28, No. C, 1958, p. 1-8.

Research output: Contribution to journalArticle

Hayashi, T, Rosenbluth, R, Satir, P & Vozick, M 1958, 'Actin participation in actomyosin contraction', BBA - Biochimica et Biophysica Acta, vol. 28, no. C, pp. 1-8.
Hayashi T, Rosenbluth R, Satir P, Vozick M. Actin participation in actomyosin contraction. BBA - Biochimica et Biophysica Acta. 1958;28(C):1-8.
Hayashi, Teru ; Rosenbluth, Raja ; Satir, Peter ; Vozick, Michael. / Actin participation in actomyosin contraction. In: BBA - Biochimica et Biophysica Acta. 1958 ; Vol. 28, No. C. pp. 1-8.
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