Accelerating effects of nonviable Staphylococcus aureus, its cell wall, and cell wall peptidoglycan

Stanley M. Levenson, Tsueng H. Chang, Dorrine Kan-Gruber, Charles Gruber, Jacob J. Steinberg, Xiaoguang Liu, Alvin Watford, Larry Freundlich, Marcos Rojkind

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We have previously reported that local application of viable Staphylococcus aureus dramatically accelerates wound healing, but viable Staphylococcus epidermidis does not. Because the S. aureus effect occurred in the absence of infection and because the cell walls of the two bacterial species differ, we hypothesized that nonviable S. aureus, its cell wall and its cell wall component(s) would accelerate healing. Nonviable S. aureus was prepared by chemical and physical means, and its cell wall and peptidoglycan was prepared from heat-killed cultures. In a large number of experiments, nonviable S. aureus (independent of the strain's protein A content), its cell wall, and peptidoglycan when instilled locally at the time of wounding each significantly increased the breaking strength of rat skin incisions (tested both in the fresh state and after formalin fixation). These agents also enhanced subcutaneous polyvinyl alcohol sponge reparative tissue collagen accumulation, generally by a factor of two. Histologic features of treated and control incisions were similar. In contrast, the reparative tissue of treated sponges contained more neutrophils, macrophages, capillaries, and collagen. These experimental data thus confirm our previous studies, as well as our hypothesis, and extend these observations of enhanced wound healing to specific fractions of the bacterial cell wall.

Original languageEnglish (US)
Pages (from-to)461-469
Number of pages9
JournalWound Repair and Regeneration
Volume4
Issue number4
StatePublished - 1996

Fingerprint

Peptidoglycan
Cell Wall
Staphylococcus aureus
Wound Healing
Collagen
Polyvinyl Alcohol
Staphylococcus epidermidis
Staphylococcal Protein A
Porifera
Cellular Structures
Formaldehyde
Neutrophils
Hot Temperature
Macrophages
Skin
Infection

ASJC Scopus subject areas

  • Dermatology
  • Surgery

Cite this

Levenson, S. M., Chang, T. H., Kan-Gruber, D., Gruber, C., Steinberg, J. J., Liu, X., ... Rojkind, M. (1996). Accelerating effects of nonviable Staphylococcus aureus, its cell wall, and cell wall peptidoglycan. Wound Repair and Regeneration, 4(4), 461-469.

Accelerating effects of nonviable Staphylococcus aureus, its cell wall, and cell wall peptidoglycan. / Levenson, Stanley M.; Chang, Tsueng H.; Kan-Gruber, Dorrine; Gruber, Charles; Steinberg, Jacob J.; Liu, Xiaoguang; Watford, Alvin; Freundlich, Larry; Rojkind, Marcos.

In: Wound Repair and Regeneration, Vol. 4, No. 4, 1996, p. 461-469.

Research output: Contribution to journalArticle

Levenson, SM, Chang, TH, Kan-Gruber, D, Gruber, C, Steinberg, JJ, Liu, X, Watford, A, Freundlich, L & Rojkind, M 1996, 'Accelerating effects of nonviable Staphylococcus aureus, its cell wall, and cell wall peptidoglycan', Wound Repair and Regeneration, vol. 4, no. 4, pp. 461-469.
Levenson, Stanley M. ; Chang, Tsueng H. ; Kan-Gruber, Dorrine ; Gruber, Charles ; Steinberg, Jacob J. ; Liu, Xiaoguang ; Watford, Alvin ; Freundlich, Larry ; Rojkind, Marcos. / Accelerating effects of nonviable Staphylococcus aureus, its cell wall, and cell wall peptidoglycan. In: Wound Repair and Regeneration. 1996 ; Vol. 4, No. 4. pp. 461-469.
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