Absolute measurement of internal volume changes inside the pulse damper: Discrepancy between delay and dwell volumes

Eduard Rogatsky, Zheng Zheng, Daniel Stein

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

We have developed a novel technique for the absolute determination of the mobile phase volume stored inside of a variable volume pulse damper at different pressures. Using an Agilent HPLC pulse damper we found a linear volume increase of ∼1 μL/bar. We found that pump pressurization is a relatively slow process and takes ∼1 min to reach 90% and takes ∼2 min to reach 99% equilibration at flow rates below 1 mL/min. During pump pressurization, column flow rate will be less than the pre-set, since part of the mobile phase is retained inside of the pulse damper. During our experiments we observed a discrepancy between data obtained by UV techniques and direct absolute measurements. This difference can be explained by a fundamental difference between the gradient delay volume and dead (dwell) volume.

Original languageEnglish (US)
Pages (from-to)1513-1517
Number of pages5
JournalJournal of Separation Science
Volume33
Issue number10
DOIs
StatePublished - Jun 1 2010

Keywords

  • Column switching
  • Delay volume
  • Dwell volume
  • Flow inconsistency
  • Pulse damper

ASJC Scopus subject areas

  • Analytical Chemistry
  • Filtration and Separation

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